KOD FX Neo - Toyobo

www.toyobo.co.jp/e/bioExample 4.Amplification from mouse tail lysatesThe amplification efficiency was then compared between various PCR enzymes usingmouse tail lysates as templates. KOD FX Neo showed greater amplification efficiencythan the other enzymes.Example 5. PCR error ratioThe error ratio of various PCR enzymes were compared by determining the sequences ofthe amplicons from human β-globin gene. The amplicons were cloned into the vectorusing TArget clone -Plus- (Code No. TAK-201) and the sequences were determined.The result indicates that the error ratio of KOD FX Neo is equal to that of KOD FX(previous version) and approximately 10 times lower than rTaq DNA polymerase.Table. Comparison of the mutation frequency of each PCR enzyme.Fidelity was measured as a mutation frequency by sequencing the PCR product. After cloning, the PCRproduct (5.7 kb of the human β-globin region), about 96 clones were selected and sequenced.JAPANTOYOBO CO., LTD.Tel(81)-6-6348-3888www.toyobo.co.jp/e/biotech_osaka@toyobo.jpCHINATOYOBO Bio-Technology, CO., LTD.Tel(86)-21-58794900.4140FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE.10