A31-5R-7 Agitator Catalog - Elliott Equipment Corporation

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ASSEMBLIESBinks gear drive agitatorsfor 55 gallon drums■ Model 31-393 Gear Drive <strong>Agitator</strong>(drive only)■ Model 31-394 Gear Drive <strong>Agitator</strong>(complete with support assembly)■ For 55 gallon drums withbuilt-in agitator31-393 31-394Furnished with:■ 1/2 HP air motor■ 15:1 gear-reducer drive (40 RPM)■ Four shaft adapter couplings: 3/8 in. dia.,7/16 in., 1/2 in. and 5/8 in. squareShipping weight: 32 lbs.Ref. Part Sheet: 77-2805Speed: Speed of the air motor is regulatedby an air adjusting valve (part no. HAV-500).The speed of the agitator shaft below thereduction gear box should not run fasterthan 30 to 40 RPM maximum.Air Supply: Air used to operate this unit shouldcome directly from a high pressure source;do not use air that has passed through aregulator. Air supply to the motor should bea minimum of 60 psi gauge pressure.An air filter and moisture trap is recommended.4
Figure 3. Purified His-PS1 Forms Na +Channels in Planar Lipid Bilayers(A and B) Proteoliposomes containing purifiedHis-PS1 and His-PS1-M146V proteins wereanalyzed by SDS gel electrophoresis followedby Coomassie staining (A) or Western blotting(B).(C) Nystatin/ergosterol-mediated fusion of His-PS1 proteoliposomes with BLM. Large nystatinchannels are observed upon fusion of proteoliposomeswith the bilayer. The activity of nystatinchannels is terminated following perfusionof the cis chamber.(D) Na + currents are shown for empty BLM(BLM) and for experiments with protein-free liposomesand proteoliposomes containing purifiedHis-PS1 and His-PS1-M146V. The dottedlines represent the zero level for the currenttraces. The direction of the current is consistentwith Na + as a current carrier (600 mM NaCl cisand 150 mM NaCl trans). For each experiment,10 s of continuous current recording is shown.Similar results were obtained in at least threeexperiments with each batch of liposomes.Ca 2+ stores independently from the InsP 3 R activation. Wefound that application of ionomycin resulted in more massiveand longer-lasting Ca 2+ signals in DKO cells than inMEF cells (Figure 4D). To estimate total ER Ca 2+ contentfrom these data, we integrated the area under the ionomycin-inducedcytosolic Ca 2+ curve for both types of cells. Onaverage, the area under the curve was equal to 30 ± 6mM3s (n = 25) for MEF cells and 55 ± 13 mM3s (n = 30)for DKO cells (Figure 4E). Thus, the size of the ionomycin-sensitiveCa 2+ pool is 1.8-fold larger in DKO cellsthan in MEF cells, consistent with overfilled ER Ca 2+ storesin DKO cells.These results led us to propose that presenilins act asER Ca 2+ leak channels that facilitate passive Ca 2+ leakacross the ER membrane. To test this hypothesis more directly,we evaluated Ca 2+ signals induced in MEF andDKO cells by thapsigargin. Application of thapsigarginblocks SERCA Ca 2+ pump activity, leading to passiveleak of Ca 2+ from the ER to the cytosol via the endogenousER Ca 2+ leak pathway. We found that application of 1 mMthapsigargin caused rapid and large cytosolic Ca 2+ elevationin MEF cells, but much smaller and delayed cytosolicCa 2+ elevation in DKO cells (Figure 4F). On average, wefound that cytosolic Ca 2+ elevation could be detected at16 ± 4 s (n = 17) after application of thapsigargin to MEFcells and at 157 ± 14 s (n = 19) after application of thapsigarginto DKO cells. To estimate the rate of endogenousCa 2+ leak in these experiments, we measured a slope ofcytosolic Ca 2+ increase in thapsigargin-exposed cells (releaserate). We found that, on average, the release ratewas equal to 1.85 ± 0.24 nM/s (n = 17) in MEF cells and0.33 ± 0.05 nM/s (n = 19) in DKO cells (Figure 4G). Thus,genetic deletion of presenilins resulted in a 5.6-fold reductionin the rate of Ca 2+ leak across the ER membrane, suggestingthat in MEF fibroblasts, presenilins account for80% of the total endogenous ER Ca 2+ leak activity.Rescue of Ca 2+ Signaling Defects in DKO FibroblastsThe defects in Ca 2+ signaling observed in DKO fibroblasts(Figure 4) provided an opportunity for a series of rescue experiments.In these experiments, DKO fibroblasts weretransfected with EGFP plasmid alone (EGFP control) orEGFP plasmid together with the presenilin expression constructsand analyzed by Fura-2 Ca 2+ imaging. In the firstCell 126, 981–993, September 8, 2006 ª2006 Elsevier Inc. 985
Page 1 and 2: A31R-7 1/05AgitatorsAgitators, Quic
Page 3: INDEXCLOSED DRUMS (55 GALLONS)MODEL
Page 7: ASSEMBLIESBinks gear drive agitator
Page 10 and 11: QUICK MIXERSBinks quick-mixersfor c
Page 12 and 13: A GITATOR ASSEMBLIESBinks C-clamp a
Page 14 and 15: A GITATORS, COVERS & ACCESSORIES■
Page 16 and 17: A CCESSORIESBinks direct drive agit
Page 18 and 19: A CCESSORIESBinks direct drive agit
Page 20: Sales and ServiceThrough a Nationwi

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