CURRICULUM VITAE - Faculty

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2004–2005 Internship at the MPI for Terrestrial Microbiology, Germany. Project title: Stable Isotope Probing (SIP) with 13 C-glucose in the intestinal tract of soilfeeding termites. Advisor: Prof. Dr. Andreas Brune. 2000–2001 Assistant laboratory technician, Nairobi Hospital (Eldoret Branch), Kenya. Responsibilities: Routine sample analysis and post-HIV-testing patient counseling. Supervisor-in-charge: Mr. Samuel Kabata. 2001 Biology and Chemistry teacher, Simat Secondary School, Eldoret, Kenya. 2000 Biology and Chemistry teacher, Ruiru High School, Thika, Kenya. Academic awards: 2008 Travel grant from the International Max Planck Research School to participate at the 12 th International Symposium on Microbial Ecology (ISME) in Cairns, Australia. 2005–2008 Ph.D study fellowship from the Deutscher Akademischer Austauschdienst (DAAD). 2004–2005 Internship fellowship from the International Max Planck Research School. Conference presentations: 2009 Annual conference meeting of the Association for General and Applied Microbiology (VAAM), Rühr-Universität Bochum, Germany. Nitrous oxide (N2O) emission and spartial separation of denitrification and nitrate ammonification in the highly compartmentalized intestinal tract of soilfeeding termites (POSTER). 2008 12 th International Symposium on Microbial Ecology in Cairns, Australia. Intestinal nitrate reduction and nitrous oxide (N2O) emission by soilfeeding termites (Isoptera: Termitidae) (POSTER). 2006 Annual conference meeting of the Association for General and Applied Microbiology (VAAM), Jena, Germany. The contribution of soil peptides and carbohydrates to the diet of soil-feeding termites, (Cubitermes spp.) (POSTER). 2004 Annual conference meeting of the Kenya Society of Microbiology, Nairobi. Characterization and identification of bacteria from the intestinal tracts of termites capable of degrading monoaromatic compounds (ORAL). Workshops and short courses: 2009 Fluorescent In Situ Hybridization (FISH) techniques in microbial ecology, MPI Marburg, Germany. 2006 Advance light microscopy techniques: an introduction to Confocal Laser Microscope (CLM), MPI Marburg, Germany. 2005 Molecular identification of microorganisms by comparative 16S-rRNA gene sequence analysis, MPI Marburg, Germany.
Professional affiliations: ! International Society for Microbial Ecology (ISME). ! Association for General and Applied Microbiology (VAAM). ! International Max Planck Research School (IMPRS). ! Kenya Society of Microbiology (KSM). Research experience: ! Working knowledge and experience with various GC systems (FID, ECD, TCD, RGD, IRMS, and RAGA) and HPLC for sugars, amino acids, and short-chained fatty acids. ! Extensively applied radio-tracers ( 14 C) and stable isotopes ( 13 C and 15 N) in various side projects. ! Anaerobic microbial cultivation and isolation skills such as Hungate and deepagar pure-culturing techniques. ! Molecular analytical techniques such as cloning (16S rRNA, pmoA, amoA, and nirS/K) phylogenetic analysis and probe design (ARB), FISH, and DNA/RNAfinger printing with TRFLP (LICOR and ABI sequencers). Published papers/manuscripts under review: 1. Ngugi K.D., Ji R., and Brune A. (2009). Stimulation of nitrogen mineralization and coupled nitrification–denitrification by soil-feeding termites (Cubitermes spp.) – a 15 N-based approach. Biogeochem., submitted. 2. Ngugi K.D and Brune A. (2009). Intestinal nitrate reduction and nitrous oxide (N2O) emission by soil-feeding termites (Cubitermes and Ophiotermes spp.). Environ. Microbiol., submitted. 3. Ngugi K.D., Tsanuo M.K, and Boga H.I. (2007). Benzoic acid degrading bacteria from the intestinal tract of Macrotermes michaelseni Sjöstedt. J. Basic Microbiol. 47:87–92. 4. Ngugi K.D., Tsanuo M.K, and Boga H.I. (2005). Rhodococcus opacus strain RW, a resorcinol-degrading bacterium from the gut of Macrotermes michaelseni. Afric. J. Biotech. 4:639–645. 5. Ngugi K.D., Fujita A., Geissinger O., Boga H.I., and Brune A. Proteolytic activities and microbial utilization of amino acids in the intestinal tract of soilfeeding termites (Isoptera: Termitidae). Appl. Environ. Microbiol., in preparation. 6. Ngugi K.D., and Brune A. Evidence for cross-epithelial transfer and excretion of ammonia in the hindgut of soil-feeding termites. Insect Physiol., in preparation. 7. Fujita A., Ngugi K.D., Miambi E., and Brune A. Cellulolytic activities and in situ rates of glucose turnover in the highly compartmentalized intestinal tract of soilfeeding termites. Appl. Environ. Microbiol., in preparation.
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