Antimicrobial activity of Calophyllum inophyllum crude extracts ...

Antimicrobial activity of Calophyllum inophyllum crude extracts obtained by pressurized liquid extraction / AsianJournal of Traditional Medicines, 2009, 4 (4)an additional outer lipid membrane. It is likely thatthe antimicrobial compounds from the extracts wereunable to penetrate this membrane and no appreciableantimicrobial activity was obtained.ConclusionPLE methanolic and n-hexane extracts of discardedCalophyllum inophyllum fruit peel demonstratedpromising activity against Staphylococcus aureusand Mycobacterium smegmatis. This indicates thatthis material is a valuable source of antimicrobialcompounds. Modern extraction methodologies suchas PLE, having shorter extraction times, will preservethese antimicrobial compounds better than conventionalsolvent extraction techniques. Further investigationsare underway to compare the performance of PLEwith conventional extraction methods and to identifythe phenolic compounds which are responsible for theobserved antimicrobial activity.References[1] Medicinal plants in the south pacific, WHO regionalpublications, Western Pacific Series No.19, 1998, 29.[2] Drug Strategies in Antibacterials, Marcel Dekker, Inc.,2000, 115.[3] Waldeback M. Pressurized Fluid Extraction, PhD thesis-Uppsala Sweden, 2005, 25.[4] Buelga CS, Williamson G, Methods in PolyphenolAnalysis, UK, TheRoyal Society of Chemistry, 2003, 2,12.[5] Dweck AC, Meadows T. Tamaru (Calophylluminophyllum) - The African, Asian, Polynesian and PacificPanacea, Blackwell Science Ltd., 2002, 1.[6] US Pharmacopoeia 30, 2007, 318-20.[7] Yimdjo MC, Azebaze AG, Nkengfack AE, Meyer AM,Bodo B, Fomum ZT, Antimicrobial and cytotoxic agentsfrom Calophyllum inophyllum, Phytochemistry 2004, 65,2789-95.146