221 Transformation of E. coli with pGAL⢠(blue colony) - EDVOTEK
221 Transformation of E. coli with pGAL⢠(blue colony) - EDVOTEK
221 Transformation of E. coli with pGAL⢠(blue colony) - EDVOTEK
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<strong>Transformation</strong> <strong>of</strong> E. <strong>coli</strong> <strong>with</strong> pGAL (<strong>blue</strong> <strong>colony</strong>)EDVO-Kit # <strong>221</strong>Important READ ME!<strong>Transformation</strong> experiments contain antibiotics which are used for the selection <strong>of</strong> transformedbacteria. Students who have allergies to antibiotics such as penicilllin, ampicillin,kanamycin or tetracycine should not participate in this experiment.Experiment ComponentsContentsComponentQuantities:Experiment # <strong>221</strong>is designed for 10groups (2-4 studentsper group).Storage: Store components A-G in the refrigerator.A <strong>Transformation</strong> LyphoCells (DO NOT FREEZE)B Supercoiled pGAL (<strong>blue</strong> <strong>colony</strong>)C Control Buffer (no DNA)D AmpicillinE X-Gal in solvent (pre-measured)**F Cell reconstitution mediumG Solvent for induction <strong>of</strong> competencyStorage: Store components listed below at Room temperature• Bottle ReadyPour Luria Broth Agar, sterile(also referred to as ReadyPour medium)• Bottle Luria Broth Medium for Recovery, sterile(also referred to as Luria Recovery Broth)• Petri plates• Plastic microtipped transfer pipets• inoculating loops (sterile)• Microtest tubes <strong>with</strong> attached lids** <strong>EDVOTEK</strong>'s solvent formulation <strong>of</strong>fers a safer alternative to the usualsolvent, N, N-dimethylformamide (DMF).All components are intended for educational research only.They are not to be used for diagnostic or drug purposes, noradministered to or consumed by humans or animals.None <strong>of</strong> the experiment components are derived from humansources.<strong>EDVOTEK</strong> - The Biotechnology Education Company ®1-800-<strong>EDVOTEK</strong> • www.edvotek.com24-hour FAX: (301) 340-0582 • email: edvotek@aol.comEVT 005077K
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