Practical experience with the use of the DR CALUXÂ® bioassay for ...

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Application of DR CALUX assay at RIKILT• Gelatin first incident discovered by DR CALUX ® ,• but DR CALUX widely used for monitoring and,• in other incidents, like ….
Food and feed incidentsBrazilian citrus pulp 1998Belgian PCB fat 1999Wild eelGerman kaolinic clay 1999Belgian cholin chloride 2002German bakery waste 2003Free range eggsPotato peels/kaolinic clay 2004Gelatin fat/Hydrochloric acid 2006
Page 1 and 2: Practical experience with the use o
Page 3: January 2006: Dioxins in recycled f
Page 7 and 8: Current use of DR CALUX ®• Test
Page 9 and 10: DR CALUX bioassay: screening assayC
Page 11 and 12: Quantitative approach: calibrationS
Page 13 and 14: JRC: Results chicken feed (n=10)Fee
Page 15 and 16: JRC: Results chicken feed (n=10)Fee
Page 17 and 18: “Recovery”Determined by differe
Page 19 and 20: “Recovery”Determined by differe
Page 21 and 22: Dose-response curves for dioxins an
Page 23 and 24: Role of CALUX-REPs in “recovery
Page 25 and 26: Determination of CALUX-REPs• Diff
Page 27 and 28: When to declare a sample suspected
Page 29 and 30: Target limits, action limits and re
Page 31 and 32: Current limitsProductTolerancelimit
Page 33 and 34: Separation of dioxins and dl-PCBs?
Page 35 and 36: Principle of the screening approach
Page 37 and 38: Adaptation of the screening approac
Page 39 and 40: Feed 5 gram (n=4)2000response (RLUs
Page 41 and 42: Improved clean-up of samples• Fat
Page 43 and 44: Conclusions based on incidents and
Page 45 and 46: Validation for citrus pulp (repeata
Page 47 and 48: Screening of citrus pulpGC/MSConten
Page 49 and 50: Future needs• Further internation
Page 51 and 52: AcknowledgementsMy collegues at RIK

Practical experience with the use of the DR CALUXÂ® bioassay for ...

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