EpiTect Bisulfite Handbook

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Kit Contents EpiTect Bisulfite Kit (48) Catalog no. 59104 Number of preps Chemical Module 48 Bisulfite Mix (aliquots for 8 reactions) 6 DNA Protect Buffer 1.9 ml RNase-Free Water 3 x 1.9 ml Purification Module EpiTect Spin Columns 48 Collection Tubes (2 ml) 96 Buffer BL* 31 ml Buffer BW (concentrate) 2 x 13 ml Buffer BD (concentrate) 3 ml Buffer EB 15 ml Carrier RNA 310 µg Handbook 1 * Contains a guanidine salt. Not compatible with disinfectants containing bleach. See “Safety Information” (page 6). Shipping and Storage The EpiTect Bisulfite Kit is shipped at room temperature (15–25°C). Upon arrival, EpiTect Bisulfite spin columns should be stored at 2–8°C. However, short-term storage (up to 4 weeks) at room temperature does not affect their performance. All buffers and the Bisulfite Mix should be stored at room temperature (15–25°C) and are stable for at least 6 months under these conditions. Reconstituted Bisulfite Mix can be stored at –20°C for up to 4 weeks. Lyophilized carrier RNA can be stored at room temperature (15–25°C) for one year. Carrier RNA can only be dissolved in RNase-free water; dissolved carrier RNA should be immediately added to Buffer BL as described on page 11. This solution should be prepared fresh, and is stable at 2–8°C for up to 48 hours. 4 EpiTect Bisulfite Handbook 04/2006
Unused portions of carrier RNA dissolved in RNase-free water should be frozen in aliquots at –20°C. Product Use Limitations The EpiTect Bisulfite Kit is developed, designed, and sold for research purposes only. It is not to be used for human diagnostic or drug purposes or to be administered to humans. All due care and attention should be exercised in the handling of many of the materials described in this text. Product Warranty and Satisfaction Guarantee QIAGEN guarantees the performance of all products in the manner described in our product literature. The purchaser must determine the suitability of the product for its particular use. Should any product fail to perform satisfactorily due to any reason other than misuse, QIAGEN will replace it free of charge or refund the purchase price. We reserve the right to change, alter, or modify any product to enhance its performance and design. If a QIAGEN ® product does not meet your expectations, simply call your local Technical Service Department or distributor. We will credit your account or exchange the product — as you wish. Separate conditions apply to QIAGEN scientific instruments, service products, and to products shipped on dry ice. Please inquire for more information. A copy of QIAGEN terms and conditions can be obtained on request, and is also provided on the back of our invoices. If you have questions about product specifications or performance, please call QIAGEN Technical Services or your local distributor (see back cover). Technical Assistance At QIAGEN we pride ourselves on the quality and availability of our technical support. Our Technical Service Departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of QIAGEN products. If you have any questions or experience any difficulties regarding the EpiTect Bisulfite Kit or QIAGEN products in general, please do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques. For technical assistance and more information please call one of the QIAGEN Technical Service Departments or local distributors (see back cover). EpiTect Bisulfite Handbook 04/2006 5
Page 1 and 2: EpiTect ® Bisulfite Handbook For c
Page 3: Contents Kit Contents 4 Shipping an
Page 7 and 8: 24-hour emergency information Emerg
Page 9 and 10: DNA Protect Buffer that contains a
Page 11 and 12: DNA Protect Buffer DNA Protect Buff
Page 13 and 14: Important Notes Yield and size of D
Page 15 and 16: Protocol: Sodium Bisulfite Conversi
Page 17 and 18: Table 2. Bisulfite Reaction Compone
Page 19 and 20: 15. Place the spin column into a ne
Page 21 and 22: � Equilibrate samples to room tem
Page 23 and 24: 9. Centrifuge the column at maximum
Page 25 and 26: � Add dissolved carrier RNA to Bu
Page 27 and 28: 7. Add 560 µl freshly prepared Buf
Page 29 and 30: Troubleshooting Guide This troubles
Page 31 and 32: ) Failure of conversion reaction Un
Page 33 and 34: Product Contents Cat. no. MagAttrac
Page 35 and 36: QIAGEN Distributors and Importers P

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