amino acid analyzer.pdf - DAFCO

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Biochrom 30+ TECHNICAL SPECIFICATIONSReproducibilityLimit of detectionArea: Better than 0.5% RSD at 10 nanomoles.Retention time: Better than 0.1%RSD9 - 15 pmoles NinhydrinTime of analysis (based on the separationof 45 physiological amino acids):Accelerated analysis:High Performance analysis:115 min injection to injection170 min injection to injectionAnalytical column:High pressure PEEK column packed with Ultropac 8 cation exchange resin. Peltierheating/cooling system.Eluent system:6 buffer system (5+1 regeneration solution) stored on the instrument at roomtemperature in graduated 1L glass bottles under nitrogen pressure.Ninhydrin reagent: Stored on the instrument at room temperature undernitrogen pressure in a 2L plastic coated glass bottle.TemperaturePhotometric detection:Sample injectionColumn temperature variable between 20°C and 99°C. Reaction Coiltemperature adjustable between 40°C and 145°C (135°C is optimum).Single flow cell with optical beam splitter.Dual channel detection at 440nm and 570nm.3 injection modes (full loop, partial loop and micro), 84 position autosampler.Sample volumes from 1µL to 5000µL. 200µL loop supplied as standard.Software:BioSys v3.0 control softwareBiochrom Alias Manager autosampler control softwareLatest version of EZChrom Elite Data Handling 21 CFR part 11 compliantDimensions and weights:Bench top Fluidics Cabinet: 48 x 59 x 57 cm, 19 x 23 x 22 inches (w x d x h)Weight:50kg, 110lbsAutosampler: 30 x 57.5 x 36 cm, 12 x 23 x 14 inches (w x d x h)Weight:21kg, 46lbsOperating Conditions:Operating temperature: 15 °C to 25 °CMaximum humidity: 80% at 25 °CRequired services:Safety SystemOxygen free nitrogen gas (99.99%) or Argon regulated to 73.5psi (5bar).Drainage facility. 240V/100V, 50Hz/60Hz, 300VA mains supply.Automatic shut-down and reaction coil flushing in the event of:n photometer lamp failuren incorrect ninhydrin / buffer / coil / nitrogen pressuresn incorrect coil and column temperaturesn power failureOrder CodeBiochrom 30+ Physiological System80 - 6000 - 50
ELUTION ORDER OF CLINICALLY SIGNIFICANTAMINO ACIDS AND DERIVATIVES ANALYZEDBY THE Biochrom 30+1 Sulfocysteine2 Phosphoserine3 Taurine4 Phosphoethanolamine5 Urea6 Aspartic acid7 Hydroxyproline8 Methionine sulfone9 Threonine10 Serine11 Asparagine12 Glutamic acid13 Glutamine14 Sarcosine15 Cysteine16 α-Aminoadipic acid17 Proline18 Glycine19 Alanine20 Citrulline21 α-Aminobutyric acid22 Valine23 Homocysteine24 Cystine25 Saccharopine26 Pipecolic acid27 Homocitrulline28 Methionine29 Cystathionine30 AlloIsoleucine31 Isoleucine32 Leucine33 Argininosuccinic acid34 NorLeucine35 Cysteine-homocysteine mixed disulfide36 Tyrosine37 β-alanine38 Phenylalanine39 δ-Aminolevulinic acid40 β-Aminoisobutyric acid41 Homocystine42 γ-Aminobutyric acid43 Ethanolamine44 Ammonia45 Hydroxylysine46 Amino Ethyl Cysteine47 Ornithine48 Lysine49 1-Methyl histidine50 Histidine51 Tryptophan52 3-Methyl histidine53 Anserine54 Carnosine55 Arginine56 HomoarginineABOUT BIOCHROMBiochrom is a world leader in amino acid analysis. The Biochrom 30+ is recognizedas the gold standard dedicated amino acid analyzer used by hospitals,pharmaceutical and industrial labs worldwide. Applications are available both forclinical analysis and screening of metabolic disorders, for drug synthesis, infusionfluids and for industrial applications in food, beverage and feedstuffs.Biochrom is a leading manufacturer of scientific instruments with over 40 year’sexperience. The Biochrom Group consists of five well-known instrument brandscovering amino acid analysis, UV/Vis spectroscopy, and microplate instrumentation.Hospitals and laboratories worldwide trust our products and we are a valued OEMpartner of many of the world's finest scientific instrumentation companies. TheBiochrom spectroscopy range includes the Novaspec, Ultrospec ® , and GeneQuant ® ,plus the Biochrom Libra and Biochrom WPA brands. Biochrom also manufacturestwo major brand names in the microplate instrumentation market - Biochrom Asysand Biochrom Anthos.All our instruments are available through a growing global network of independentdistributors, backed by our commitment to customer support. Biochrom is a HarvardBioscience Company.EZChrom Elite is a trademark of Agilent TechnologiesWindows is a trademark of MicrosoftHP is a trademark of Hewlett PackardUltrospec and Genequant are registered trademarks of Biochrom1Differential diagnosis of (inherited) amino acid metabolism or transport disorders.W. Blom & J.G.M.Huijmans, Amino Acids (1992) 2:25-672Vademecum Metabolicum Manual of Paediatrics. J. Zschocke & G. Hoffmann, 2ed.Milupa GmbH 20043Rapid Analysis of Homocysteine Levels. A.Lolia & S. Bee,Biochrom Application note B30.5.11
Page 1 and 2: THE BIOCHROM 30+AMINO ACID ANALYSIS
Page 3 and 4: amino acid analyzersA DEDICATED SYS
Page 5 and 6: amino acid analyzersSOFTWAREBiochro
Page 7 and 8: PLASMA ANALYSISA full screening is
Page 9: TOTAL HOMOCYSTEINE ASSAYElevated pl

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