14. Measuring Feed Intake - Department of Animal Sciences ...

Alkane analysis• Alkanes in a sample are separated bysaponification with KOH or extraction with nonpolarsolvents e.g. Heptane / hexane (safer)• This removes fatty acids as soaps or removes thelipids, both of which can interfere with the analysis• The alkanes are present in the non-lipid, orunsaponifiable fraction and are measured by GC