1. Infectious Pathogen Detection - Seegene

Internal control
(360bp) van A
(250bp) van B
2.1 VRE ACE Detection
Seeplex® VRE ACE Detection detects the vanA and vanB genes which cause vancomycin-resistance in Enterococci
cultured in Enterococcosel broth/agar.
Enterococci are normally indigenous bacteria found in the GI tract and female genitals, but recently they were recognized as major
pathogens causing urinary tract infections and sepsis by nosocomial infection. Vancomycin, which is used in the treatment of enterococci
infection, is a drug that has an antibacterial effect by forming a complex with the D-alanyl-D-alanine terminal of the peptidoglycan precursor
to block transglycosylation and transpeptidation during the synthesis of the cell wall of Gram-positive cocci. However, vancomycinresistant
enterococci (VRE) tend to multiply rapidly due to the high frequency and continuous use of vancomycin.
Seeplex® VRE ACE Detection is a PCR method that incorporates DPO TM technology with high sensitivity and specificity, and is a product
that can test for the vancomycin-resistant vanA gene and the vanB gene of enterococci in one test.
Fig 1. Band information
M 1 2 3 N P S
Fig. 2 & Table 1. Example
M
M 1 2 3 N P S
M: VRE size marker | 1: vanA positive sample |
2: vanB positive sample | 3: Negative sample |
N: Negative control | P : Positive control |
S: 100 bp ladder
vanA
P
vanB
P
van A-positive control
(1010bp)
van B-positive control
(750bp)
Internal control
(520bp)
N 1 2 3 4 5 6 7 8 9 10 11 M
M: VRE size marker | P: Positive control | 1-11: Stool samples cultured in enterococcosel
broth | N: Negative control
Clinical
Sample
Result
Clinical
Sample
Result
1
*_
7
vanA
2
vanA
8
vanA
3
vanA
9
vanA
4
*_
10
vanA
5
vanA
11
*_
6
vanA
*Not detected.
Fig. 2 & Table 1. Amplified PCR products using Seeplex® VRE ACE Detection
were run on agarose gel and vanA gene was detected in 8 of 11 patient
samples.
2. Drug Resistance Detection
Available specimen
Enterococci cultured
in Enterococcosel broth/agar
Compatible instruments
ScreenTape® System (Lab901),
MCE-202, “MultiNA” (Shimadzu)
Internal
control
van A
van B
Fig. 3 & Table 2. Example
Top Marker
VanA-positive control
VanB-positive control
Internal Control
Clinical
Sample
1
2
3
4
VanA
VanB
Bottom Marker
®
VRE ACE Detection
Product Cat. No. Size
Seeplex® VRE ACE Detection VR1210Y 50 rxns
1 2 3 4 5 6 7
1~7: Stool samples cultured in enterococcosel broth (ScreenTape® System,
Lab901 Limited)
Result
vanA vanA
vanB
*- *-
*-
Clinical
Sample
5
6
7
Result
vanA
*Not detected.
Fig. 3 & Table 2. Amplicied PCR products using Seeplex® VRE
ACE Detection and result analysis. In this example, vanA
and vanB genes were successfully detected in 4 patient
samples. (Rapid & easy interpretation using ScreenTape®
System)
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