Testing of disinfectants
Testing of disinfectants
Testing of disinfectants
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Determination <strong>of</strong> phenol coefficient1- Rideal Walker method:– Organism: Salmonella typhi suspension.– Temp: 20°C– Subcultures are performed from both the testand phenol at intervals <strong>of</strong> 2.5, 5, 7.5 and 10minutes.– The plates are incubated for 48-72 hours at37°C.– Growth or no growth– R.W phenol coefficient = That dilution <strong>of</strong>disinfectant which disinfects the suspension in agiven time is divided by that dilution <strong>of</strong> phenolwhich disinfects the suspension in same time
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