Pitfalls of Acylcarnitine Analysis - MetBio.Net

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Sheffield SCBU• Contamination on blank card• All samples (7) from SCBU over 2 weekperiod reviewed – all samples and blankcard normal• SCBU discarded all stored cards andimproved storage conditions
ContaminationProblems• Microtitre plates.New batch <strong>of</strong> Greiner plates- Every specimen on run hadC8 <strong>of</strong> ~1.3 µmol/l. Changed to Corning plates.• Transfer plates• Pipette tips.• Instrument failures – Backup essential
Page 1 and 2: Pitfalls of AcylcarnitineAnalysisAn
Page 3 and 4: Analytical ProblemsIsobaric interfe
Page 5 and 6: Clinical Referral• Family contact
Page 7 and 8: Screening Specimen: DerivatisedOcta
Page 9 and 10: ActionDerivatise all elevated C8 sp
Page 14 and 15: Isobaric CompoundsAcylcarnitine MRM
Page 16 and 17: Initial Specimen•VF 14 years•Co
Page 18 and 19: Low Free Carnitine• Included in t
Page 20 and 21: Clincal importance of CTDdiagnosisM
Page 22 and 23: Problems with early C3sCB Day 1 Day
Page 24 and 25: Maternal diagnosis• 2, Carnitine

Pitfalls of Acylcarnitine Analysis - MetBio.Net

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