Phospholipid Removal Solutions - Phenomenex

An Improved Phospholipid Removal SolutionRemove All Classes of PhospholipidsLysophosphatidylcholines and phosphatidylcholines both contribute to matrix effects. Remove allclasses of phospholipids using Phree Phospholipid Removal Products.1.5e6Intensity, cpsIntensity, cps1.4e61.5e61.3e61.4e61.2e61.3e61.1e61.2e61.0e61.1e69.0e51.0e68.0e59.0e57.0e58.0e56.0e57.0e55.0e56.0e54.0e55.0e53.0e54.0e52.0e53.0e51.0e52.0e501.0e5acetonitrile with 1 % formic acidColumn: Kinetex ® 2.6 µm C18 100 ÅDimensions: 50 x 2.1 mmPart No.: 00B-4462-ANMobile Phase: A: 0.1% Formic acid in WaterB: 0.1% Formic acid in MethanolSupelco ® HybridSPE ® 60Plasma Cleanup: 100 µL plasma plus 300 µLPhenomenex ® Gradient: Time (min) % BPhree0 600.5 95Agilent ® Captiva NDLipids15.5 9515.51 60Flow Rate: 400 µL/minDetection: Mass Spectrometer (MS) @ 425 °C;184 amuTemperature: 22 °C0 12 3 4 5 6 7 8 9 10 min00 12 3 4 5 6 7 8 9 10 min~ 25x Zoom4.8e4Intensity, cpsIntensity, cps4.4e44.8e44.0e44.4e43.6e44.0e43.2e43.6e42.8e43.2e42.4e42.8e42.0e42.4e41.6e42.0e41.2e41.6e480001.2e44000800004000Phree is most effective atremoving all classes of phospholipids1 2 3 4 5 6 7 8 9 10 minApp ID 200150Phospholipid profile monitored1using m/z2184-1843 4 5 6 7 8 9 10 minAgilent is a registered trademark of Agilent Technologies Inc. Captiva is a trademark of Agilent Technologies, Inc.Supelco and HybridSPE are registered trademarks of Sigma-Aldrich Co.Comparative separations may not be representative of all applications.6Phenomenex lWEB: www.phenomenex.com