FSH IRMA

FSH IRMA – COATED TUBECatalog Number: 07-246102 (100 Tubes)ASSAY:SAMPLE:STANDARDS:TEST PRINCIPLEImmunoradiometric100 µl serum or plasma0, 2.5, 5.0, 10, 25, 50, 100, 200 mIU/mlThe ImmuChem TM FSH-CT IRMA kit, employs an immunoradiometricassay technique to quantitate human follicle stimulatinghormone in either a serum or plasma sample. Inimmunoradiometric assays, two antibodies are generatedagainst different portions of the same antigen. In theImmuChem IRMA, one antibody is bound to a solid phasesupport (the tube), while the other antibody is labeled with 125 I.Thus when an antigen is present, it simultaneously binds bothantibodies in a “bridge” or “sandwich” fashion. This entirecomplex remains bound to the tube, while the unboundradioisotope remains in solution and is later decanted. Thebound radioactivity may then be quantitated using a gammacounter.In this procedure, the sample and buffer are pipetted into theantibody coated tube and incubated for forty-five (45) minutesat 37°C. The tubes are emptied, washed and filled with thecomplimentary antibody labeled with 125 I. After a second fortyfive(45) minute incubation period at 37°C, the tubes areemptied and washed twice to remove unbound 125 I labeledantibody. The emptied tubes are counted in a gamma counterand a standard curve is constructed from which unknownconcentrations of follicle stimulating hormone can becalculated.PRECISIONINTRA-ASSAY PRECISION (mIU/ml)LEVEL I LEVEL II LEVEL IIIn = 10 10 10mean = 13.1 35.0 68.6SD = 0.34 1.4 3.7%CV = 2.0 4.0 5.4INTER-ASSAY PRECISION (mIU/ml)LEVEL A LEVEL B LEVEL Cn = 10 10 10mean = 13.7 37.1 72.4SD = 1.1 2.2 3.3%CV = 8.0 5.9 4.6SPECIFICITY OF THE ANTISERUMThe following ligands have been checked for cross-reactivitywith this system. The percentages indicate the cross-reactivityobtained from the addition of 1000 mIU/ml of the ligands.COMPOUND% CROSS REACTIONhuman Follicle Stimulating Hormone 100.00human Luteinizing Hormone