Proceedings of paspk.org Volume42-4 - Pakistan Academy of ...

253 Proc. Pakistan Acad. Sci. Amina 42(4):253-259.2005Mumtaz et alDETERMINATION OF TRIPROLIDINE-HCl BYSPECTROPHOTOMETRIC METHOD IN PURE ANDPHARMACEUTICAL PREPARATIONS USINGDICHLORONITROBENZENE AS A NEW CHROMOGENIC REAGENTAmina Mumtaz, Asrar A. Kazi*,Tehseen Aman, M. Usman Sabri and Fauzia NoureenApplied Chemistry Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex,Ferozepur Road, Lahore-54600, PakistanReceived August 2005, accepted October 2005Communicated by Dr. M. Iqbal ChoudharyAbstract: In the present study it is demonstrated that H 1- blockers such as triprolidine–HCl can bedetermined by a very simple, sensitive and accurate spectrophotometric procedure. The methodconsisted of interaction of triprolidine-HCl with dichloronitrobenzene in alkaline medium. Absorbanceof resulting orange colour was measured at 440 nm .The reaction turned out to be selective for triprolidine–HCl with 0.005 mgml -1 as the visual limit of identification and provided a basis for a newspectrophotometric determination. The reaction obeyed Beer’s law from 0.05 mg to 0.15 mgml -1 fortriprolidine–HCl and the relative standard deviation was 0.60 %. The quantitative assessment of otherdrugs was also studied.Keywords: Triprolidine–HCl determination, dichloronitrobenzene, colour stability, analytical pharmacyIntroductionTriprolidine hydrochloride (Fig.1) is a pyridinederivative with the properties of antihistamine. It isa potent histamine H 1-receptor antagonist (H 1-blocker), with a rapid onset and long duration action,almost up to 12 hours. It is probably effective forthe symptomatic treatment of seasonal and perennialallergic rhinitis, vasomotor rhinitis, allergicconjunctivitis due to allergens, foods and preventionof allergic reactions to blood or plasma [1]. Themost common side effects are sedation, dizziness,incoordination, gastrointestinal disturbances, nausea,vomiting and diarrhea. It may also produce blurredvision, dryness of mouth, tightness of chest, blooddisorders including agranulocytosis andhaemolyticanaemia [2].In view of the importance of antihistamineconsiderable analytical work has been carried out.*Author for correspondenceIn the reversed phase HPLC [3,4] and HPLCphotodiode procedures [5], 12.5 cm Nucleosil 100t – 5C 18bonded phase column with a mobile phaseconsisting of methanol acetonitrile was used. A0.01M potassium dihydrogen phosphate solutionwas employed to adjust the pH to 6.8 [3] and UVdetection was carried out at 254 and 280 nm [4]. Inanother HPLC method different UV absorptioncharacteristics of triprolidine hydrochloride have beenused to facilitate its determination in a mixture [6]. Awavelength switching programme has to beemployed in this procedure. In the micro extractioncapillary GC procedure [7] headspace solid-phaseextraction was carried out followed by capillary gaschromatography with flame ionization detection. Therecoveries in blood extraction were 4 - 51 foldslower than those in urine extraction. In the capillaryelectrophoresis procedures [8,9] the quality ofseparation was dependent upon the sample diluentused [8] and only basic drugs are screened fromblood [9] while most of the spectrophotometric