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M.Ajitha * et al. /International Journal Of Pharmacy&Technologyk: R=H, R 1 =H, R 2 =C 8 H 5 N 2 O(5-phenyl-1,3,4-oxadiazole)l: R=CH 3 , R 1 =H, R 2 = C 8 H 5 N 2 O(5-phenyl-1,3,4-oxadiazole)m: R=Cl, R 1 =H, R 2 = C 8 H 5 N 2 O(5-phenyl-1,3,4-oxadiazole)n: R=Br, R 1 =H, R 2 = C 8 H 5 N 2 O(5-phenyl-1,3,4-oxadiazole)o: R=H, R 1 =CH 3 , R 2 = C 8 H 5 N 2 O(5-phenyl-1,3,4-oxadiazole)p: R=H, R 1 =H, R 2 =C 8 H 5 N 2 S(5-phenyl-1,3,4-thiadiazole)q: R=CH 3 , R 1 =H, R 2 = C 8 H 5 N 2 S(5-phenyl-1,3,4-thiadiazole)r: R=Cl, R 1 =H, R 2 = C 8 H 5 N 2 S(5-phenyl-1,3,4-thiadiazole)s: R=Br, R 1 =H, R 2 = C 8 H 5 N 2 S(5-phenyl-1,3,4-thiadiazole)t: R=H, R 1 =CH 3 , R 2 = C 8 H 5 N 2 S(5-phenyl-1,3,4-thiadiazole)The structure target compounds are depicted in Figure-1. Isatin hydrazones were obtained from an appropriateisatin in alcohol along with dropwise addition of hydrazine hydrate [6]. Isatin-3-[N 2 -(chloroacetyl)] hydrazonewere synthesized by refluxing isatin hydrazone with chloro acetyl chloride in dry benzene under anhydrousconditions using calcium chloride guard tube for 2h [7]. Isatin-3-[N 2 -(heteryl-2-thioacetyl)] hydrazones (a – t)were synthesized by refluxing Isatin-3-[N 2 -(chloroacetyl)] hydrazone with an appropriate heteryl-2-thione(Benzimidazole-2-thione; 4,5-diphenyl imidazole-2-thione; 5-phenyl-1,3,4-oxadiazole-2-thione, and 5-phenyl-1,3,4,-thiadiazole-2-thione) in dry pyridine for 30 min [8].All the newly synthesized compounds werecharacterized by physical, spectral (IR, PMR) and elemental analysis. Antibacterial and antifungal screening wascarried out using the dilution agar technique [9,10]. Test organisms Bacteria: Bacillus subtilis, Staphylococcusaureus, Escherichia coli, Bacillus macarances. Fungi:Pencillium minioluteum, Fusarium solani. Evaluation ofantimicrobial activity as follows.Since the compounds were poorly water soluble, they were dissolved in propylene glycol. In order to ensure thatthe solvent had no effect on bacterial growth, an inoculated control test was performed with only propylene glycolat the same dilutions used in our experiment and found inactive in culture media. The compound suspensions wereIJPT | March-2011 | Vol. 3 | Issue No.1 | 1449-1455 Page 1451