255 - Frederiksen

Purification & Size Determination of gfp & bfpEDVO-Kit #255Components & RequirementsExperiment ComponentsThere is enough of each samplefor six (6) groups sharing threepolyacrylamide gels.Store componentsA-D, F, G in thefreezer.Component StorageA Cell Extract containing green protein (gfp) FreezerB Cell Extract containing blue protein (bfp) FreezerC Column Elution Buffer (10x) FreezerD Protein Molecular Weight Standards FreezerE Dry Matrix Room temperatureF Protein Denaturing Solution FreezerG 50% Glycerol Solution Freezer• Tris Glycine SDS Electrophoresis Buffer Room temperature• Protein InstaStain® sheets Room temperature• Protein Plus Stain Room temperature• Chromatography Columns Room temperatureNone of the components have been prepared from humansources.RequirementsAll components areintended for educationalresearch only. They are notto be used for diagnostic ordrug purposes, nor administeredto or consumed byhumans or animals.THIS EXPERIMENT DOESNOT CONTAIN HUMANDNA. None of the experimentcomponents are derivedfrom human sources.• Vertical Gel Electrophoresis Apparatus (optional)• D.C. Power Source (optional)• Automatic Micropipet (5 -50µl) and Tips(Cat. #638 Fine Tip Micropipet Tips recommended)• Balance• Ice Buckets and Ice• Long U.V. lamps (Cat. #969)• Ring stands and column clamps• 1ml pipets and pipet pumps• Microtest tubes• Polyacrylamide gels (3)*• Glacial acetic acid• Methanol• Distilled water*Polyacrylamide gels are not included for the electrophoresis part of thisexperiment. The experiment is designed for two student groups to share a gel.A total of 3 polyacrylamide gels (Precast polyacrylamide gels, Cat. #651) areEDVOTEK - The Biotechnology Education Company®1-800-EDVOTEK • www.edvotek.comFAX: (301) 340-0582 • email: info@edvotek.comEVT 005077K