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Results and DiscussionNarender Prasad.D * et al. /International Journal Of Pharmacy&TechnologyPhytochemical screening: Qualitative Phytochemical screening for different extracts of Mesua ferrea leavesrevealed the presence of steroids, terpenoids, glycosides, tannins, alkaloids, phenols and carbohydrates. Themethanol, hydro-alcoholic extracts contain saponins but hexane, ethyl acetate extracts do not contain saponins andthe all extracts are not containing flavonoids, quinines, amino acids and oils. The results of phytochemicalscreening were showed in the Table 1.Table 1: Phytochemical constituents present in different extracts of Mesua ferrea leaves.Name of thePhytochemicalsHexaneextractExtracts of Mesua ferrea leavesEthyl acetate Methanolextract extractEthanol(70%)Phenols++++Phytosterols++++Terpenoids + + + +Glycosides + + + +Saponins - - - +Flavonoids - - - -Tannins - + + +Carbohydrates + + + +Alkaloids + + + +Amino acids - - - -Oils - - - -Quinones - - - -+ = Present, – = AbsentIJPT | Dec-2011 | Vol. 3 | Issue No.4 | 3624-3630 Page 3627
Evaluation of antibacterial activity:Narender Prasad.D * et al. /International Journal Of Pharmacy&TechnologyAmong all the tested Mesua ferrea leaves extracts 70% ethanol and methanol extracts have shown significant antibacterial activity as compared to that of hexane and ethyl acetate extracts. All the extracts showed good zone ofinhibition against gram negative bacteria than gram positive bacteria. All the extracts of Mesua ferrea leaves hadproduced a minimum zone of inhibition against some tested bacterial species at a dose of 150 and 300 µg/100µl.The hexane extract do not showed zone of inhibition against Bacillus pumillis. All extracts showed good zone ofinhibition at a dose of 600 and 1200 µg/100µl. The highest zone of inhibition was showed by hexane and methanolextracts against Klebsiella pneumonia and Pseudomonas aeruginosa at the dose of 1200 µg/100µl. further studywill be carrying out to isolate the active compounds from the extracts through column chromatography. The resultsof Antibacterial activity were showed in Table 2.Table 2: Antibacterial activity of Mesua ferrea leaves extracts.Name of the dosezone of inhibition # (diameter in mm)(µg/cup)Mesua ferrea leaves Extractgram +vegram -veB.p. B.m S.e S.p E.c P.a K.p S.tHexaneEthyl acetateEthanol (70%)150300600120015030060012001503006001200------68--710-6811-5710--69--6857912-5710-6810-6810-6811681012691215-5810791216791214681013-68105791257912-68956810-6810Methanolic 150300-7--57----69-6-7IJPT | Dec-2011 | Vol. 3 | Issue No.4 | 3624-3630 Page 3628
Page 6 and 7: Narender Prasad.D * et al. /Interna

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