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J.Venkateswararao * et al. /International Journal Of Pharmacy&Technology700600500400618.1411.6510.2563.8465.3300200100052.1 38.3 41.2 43.5 39.7glucose sucrose maltose lactose fructoseEnzyme activity(U/ml)cell dry weight(mg/ml)Fig.2 Effect of various carbohydrates on enzyme activity and cell dry weight of F5.To optimize the nitrogen content various sources of nitrogen were used as shown in Fig. 3 and 4.Fig.3 Effect of various nitrogen sources on enzyme activity and cell dry weight of PW3.Fig.4 Effect of various carbohydrates on enzyme activity and cell dry weight of F5.The rapidly metabolizable complex nitrogen sources are widely used for studying the growth and productformation characteristics of microorganisms in the laboratory as they provide a good source of various amino acids,IJPT | Dec-2011 | Vol. 3 | Issue No.4 | 3735-3744 Page 3740
J.Venkateswararao * et al. /International Journal Of Pharmacy&Technologyvitamins, minerals and other unknown growth factors to sustain good growth of microorganism. The nitrogen levelwas maintained the same in all cases as 177.5mg of nitrogen/100ml of media. Inorganic nitrogen sources likeammonium sulfate, ammonium nitrate, ammonium chloride, and potassium nitrate showed their positive effect oncell growth as well as on ribonuclease production. Both PW3 and F5 were shown good growth and considerableamount of ribonuclease production when grown on beef extract, peptone. PW3 produced higher amount of enzyme(534.8 U/ml) when we used the combination of beef extract with peptone as nitrogen source. It was found that theamount produced by peptone and beef extract were 458.3U/ml and 439.4U/ml respectively. Similarly, F5 producedhigher amount of enzyme (568.2 U/ml) when we used the combination of beef extract with peptone as nitrogensource. It was found that the amount produced by peptone and beef extract were 495.7U/ml and 475.8U/mlrespectively. Both of the above instances indicate that combined use beef extract and peptone gave the best resultsand was chosen as the suitable nitrogen source for ribonuclease production.Effect of pH and temperatureThe maximum enzyme production was studied at pH range of 3.5-6.5 for fungi and 4.5-7.5 for bacteria, found thatthe optimum pH is 6.0 for the fungal strain and that is 7.0 for the bacterial.Similarly studied the effect of temperature for enzyme production and was found to be 30 0 C and 37°C respectively(Fig.7 and 8).Fig.5 Effect of pH on enzyme activity and cell dry weight of PW3.IJPT | Dec-2011 | Vol. 3 | Issue No.4 | 3735-3744 Page 3741
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