Microalgae as Biofuel: Engineering a Super Algae - COSMOS
Microalgae as Biofuel: Engineering a Super Algae - COSMOS
Microalgae as Biofuel: Engineering a Super Algae - COSMOS
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Procedure (2): Find transcriptionfactor for ACC<strong>as</strong>e gene usingAffinity Chromatography• Transcription factors are proteinsthat bind to the enhancer or promoterregions of a given gene andoverexpress the gene• Attach PCR product molecules tosolid matrix beads in a column• Pour extract of c. reinhardtii cellsover the beads• The promoter’s protein will bind tothe beads (all other proteins flowthrough column)• W<strong>as</strong>h beads with buffer that rele<strong>as</strong>esproteinsFigure 15
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