Full Brochure - Langford Veterinary Services

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EndocrinologyMicrobiologyENDOCRINOLOGY SAMPLE TURNAROUND INFORMATIONACTH stimulation test(Canine)ACTH stimulation test(Feline)Dexamethasonesuppression testSerum/Hep plasma 1-3 days Time: 0 and 2 hoursSerum/Hep plasma 1-3 days Time: 0, 60 and 90 minutesSerum/Hep plasma 1-3 days Time: 0, 3 and 8 hoursFree T4 (ED) Serum Usually 5 -7 days Method: Equilibrium dialysisFructosamineSerum(not serum gel)2-4 daysTotal T4 Serum Same dayTotal T4 + TSH (Canine) Serum 1-2 daysTSH (Canine) Serum 1-2 daysUrine cortisol: creatinineratioSerum1-2 daysThis laboratory processes appropriate samples from companion, farm and exotic animals (and the environment) whichmay be infected, in a carrier state or harbouring potentially infectious bacteria. Experienced technicians have an array oftechniques for identification of organisms.The laboratory employs microscopy and Wood’s lamp fluorescence for the detection of fungal elements in hair samples fromcompanion, farm and exotic animals. Samples of hair, skin, nail and biopsy material where mycosis is suspected are alsocultured for the presence of dermatophytes and differentiation from non-dermatophytes.The laboratory also handles high numbers of samples, mostly faecal and hair/skin samples for the presence of endo– andecto– parasites. The Bacteriology laboratory participates in the national quality assurance scheme (NEQAS) to ensure theongoing reliability and quality of results.BACTERIOLOGY SAMPLE TURNAROUND INFORMATIONBlood culture Up to 7 days Per pair of bottlesCulture & sensitivity Swab/Tissue/Fluid 3-5 days Aerobic culture / Anaerobic culture(site-dependent)/ Antibiotic sensitivities(where relevant)Culture & sensitivity(urine)Urine 3 days Aerobic culture / Antibiotic sensitivities(for significant isolate(s))Faecal screen Faeces 2-4 days Bacterial culture & parasitologyMycobacterial screening(AAFB)Smear Next day ZN stainMYCOLOGY SAMPLE TURNAROUND INFORMATIONFungal culture(Non-dermatophyte)Ringworm diagnosis(Dermatophytes)5 daysHair/skin 10-12 days Wood’s lamp, microscopy & dermatophytecultureECTOPARASITES SAMPLE TURNAROUND INFORMATIONSkin parasites Skin scrape Next dayPARASITOLOGY SAMPLE TURNAROUND INFORMATIONImmunologyIMMUNODIAGNOSTIC TESTS SAMPLE TURNAROUND INFORMATIONAntinuclear antibody(Dog, cat, horse)Blood cross match(Dog, cat, horse)Coombs’ test(Dog - IgG, IgM, C3)(Cat - IgG, IgM)(Horse - IgG, IgM)Serum Approx 1 week Full titration and pattern descriptionEDTA blood & serumfrom donors andrecipientSame or next dayEDTA blood Same or next day Full titration of polyvalent and class-specificreagents (IgG, IgM, C3) at both 4°Cand 37°CFeline blood typing (Cat) EDTA blood Same or next dayRheumatoid factor(Dog, Cat, Horse)Serum or synovialfluid in EDTAApprox 1 weekFull titrationCryptosporidium Faeces Next day Modified ZN stainFaecal parasitology Faeces Same or next dayFaecal screen Faeces Bacterial culture & parasitologyTritrichomonas foetus PCRFaeces minimum0.5g5-7 days Includes internal amplification control toavoid false negativesSEROLOGY SAMPLE TURNAROUND INFORMATIONAspergillus Serum 2 weeks Precipitation test (Antibodies)Cryptococcus Serum 10-15 days AntigenLeptospira Full screen Serum 10-15 days L. canicola, ictero, bratislava, grippotyphosa,hardjo, pomonaNeospora Ab Serum/CSF 3-5 days IFAToxoplasma IgG + IgM(Canine/Feline)Serum/CSF 3-5 days IFALangford Veterinary Services Dedicated to Excellencewww.langfordvets.co.uk/diagnostic_laboratories.htm 7
VirologyMolecular Diagnostic Unit (QPCR)This laboratory’s expertise is in feline virology.The technicians are experienced and skilled in the many serological and culture methods for most of the major feline viruses.VIRUS ISOLATION SAMPLE TURNAROUND INFORMATIONFeline Herpes Virus (FHV)/Feline Calici Virus (FCV)isolationSwab in VTM 4-8 days IsolationPox virus isolation Swab in VTM 4-10 days IsolationVIRUS ISOLATION ≠ PCRs SAMPLE TURNAROUND INFORMATIONFCV/FHV isolation+ FHV QPCRFCV/FHV isolation +Chlamydophila felis QPCRFCV/FHV isolation + FHV+ Chlamydophila felis QPCRSerologySwab in VTMSwab in VTMSwab in VTM4-8 days1-3 days4-8 days1-3 days4-8 days1-3 daysIsolation and+ FHV QPCRIsolation and Quantitative PCRIsolation and Quantitative PCRSEROLOGY SAMPLE TURNAROUND INFORMATIONViral Screen EDTA 1-3 days FIV Ab (ELISA), FeLV Ag (ELISA)Coronavirus Ab (ELISA)FIV Ab EDTA Same or next day ELISAFeLV Ag EDTA Same or next day ELISAFIV Ab + FeLV Ag EDTA Same or next day ELISACoronavirus (FIP) Ab Serum 1-3 days ELISAChlamydophila felis Ab Serum 1-3 days IFAFCV Ab Serum 1-2 weeks Viral neutralisationFHV Ab Serum 1-2 weeks Viral neutralisationPox Virus Ab Serum 1-2 weeks Viral neutralisationArthropod-borne DiseaseINFECTIOUS DISEASES PCR’S SAMPLE TURNAROUND INFORMATIONAnaplasma phagocytophilum EDTA 1-3 days QPCRBabesia spp EDTA 1-3 days PCRBartonella henselaeBartonella sppBorrelia sppEDTA or tissuesampleEDTA or tissuesampleEDTA +/or synovialfluid1-3 days QPCR1-3 days PCR1-3 days Synovial fluid in EDTAEhrlichia spp EDTA 1-3 days PCRLeishmaniaEDTA, conjunctivalswab or tissuesample1-3 days QPCRLeishmania Antibody ELISA Up to 7 days PCRThe Molecular Diagnostic Unit was the first veterinarydiagnostic laboratory in the UK to offer state of the artquantitative real-time PCR (QPCR) assays.They give our clinical pathologists much more information than can be derived from conventional PCR, allowing them toprovide a better diagnostic service to the veterinary surgeon. There are discounts when you use these tests in combinationwith virology, and serology, and if multiple tests are run. See our Price Lists for further information.INFECTIOUS DISEASE SAMPLE TURNAROUND INFORMATIONBordetella bronchiseptica(Canine/Feline)Throat swab or trachwash, BAL5-7 days Quantitative PCR*Canine haemoplasma EDTA blood 1-3 days Detects and quantifies two caninehaemoplasma speciesChlamydophila felis Dry or VTM swab 1-3 days Quantitative PCR*Feline calicivirus (FCV) Dry or VTM swab 5-7 days Quantitative PCR*Feline haemoplasma EDTA blood 1-3 days Detects and quantifies three felinehaemoplasma speciesFeLV DNA (provirus)EDTA blood or bonemarrow1-3 days Quantitative PCR. Bone marrow sampleshould be an aspirate collected in anEDTA tubeFeLV + FIV EDTA blood 1-3 days Quantitative PCR*FHV Dry or VTM swab 1-3 days Quantitative PCR*FIV DNA (provirus) EDTA blood 1-3 days Quantitative PCR*Mycoplasma felisThroat swab, ocular 5-7 days Quantitative PCR*swab, trach wash orBALTritrichomonas foetus Faeces min 0.5g 5-7 days Quantitive PCR**Includes internal amplification control to reduce false negativesFELINE GENETIC DISEASE PCRs SAMPLE TURNAROUND INFORMATIONBurmese GM2Feline hypertrophic (HCM)cardiomyopathyFeline polycystic kidneydisease (PKD)Feline pyruvate kinasedeficiency (PKDef)GSD IVProgressive retinal atrophy(PRA)Blood typeCoat Colours – agouti,amber, burmese, siamesecolour point, chocolate,cinammon, diluteLong HairEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swabEDTA blood orbuccal swab3-4 days For burmese3-4 days For Ragdoll and Maine Coon breeds only3-4 days For Persian and related breeds3-4 days For Abyssinian, Somali and related breeds3-4 days For Norwegian Forest Cats3-4 days For Abyssinian, Somali and related breedsFELINE GENETIC PCRs SAMPLE TURNAROUND INFORMATION3-4 days For all breeds except Ragdoll and TurkishAngora3-4 days All breeds3-4 days All breedsLangford Veterinary Services Dedicated to Excellence www.langfordvets.co.uk/diagnostic_laboratories.htm 109
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Full Brochure - Langford Veterinary Services

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