Carbonic Anhydrase and Small Molecule Binding Assay Using a ...

Good Lab Practice1. Prior to adding protein targets to Epic ® microplates, avoid exposing microplates to airfor extended period of time. It is recommended to use the microplates within 6 hoursafter opening the pouch for best results.2. Required soaking time before taking measurements is important for stabilizing sensors,thus reducing signal variability.3. Always use the same source of reagents when running multiple assays.4. Watch out for bubbles.5. Do not spin microplate upside down once protein has been added.Furosemide Binding to Immobilized Carbonic AnhydraseFor additional product or technical information, please visitwww.corning.com/lifesciences or call 1.800.492.1110. Outsidethe United States, please call +1.978.442.2200.Corning IncorporatedLife SciencesTower 2, 4th Floor900 Chelmsford St.Lowell, MA 01851t 800.492.1110t 978.442.2200f 978.442.2476www.corning.com/lifesciencesWorldwideSupport OfficesA S I A / P A C I F I CAustralia/New Zealandt 0402-794-347Chinat 86 21 2215 2888f 86 21 6215 2988Indiat 91 124 4604000f 91 124 4604099Japant 81 3-3586 1996f 81 3-3586 1291Koreat 82 2-796-9500f 82 2-796-9300Singaporet 65 6733-6511f 65 6861-2913Taiwant 886 2-2716-0338f 886 2-2516-7500Corning and Epic are registered trademarks of Corning Incorporated, Corning, NY.All other trademarks in this document are the property of their respective owners.Corning Incorporated, One Riverfront Plaza, Corning, NY 14831-0001E U R O P EFrancet 0800 916 882f 0800 918 636Germanyt 0800 101 1153f 0800 101 2427The Netherlandst 31 20 655 79 28f 31 20 659 76 73United Kingdomt 0800 376 8660f 0800 279 1117All Other EuropeanCountriest 31 (0) 20 659 60 51f 31 (0) 20 659 76 73L A T I N A M E R I C ABrasilt (55-11) 3089-7419f (55-11) 3167-0700Mexicot (52-81) 8158-8400f (52-81) 8313-8589© 2012 Corning Incorporated Printed in U.S.A. 2/12 POD CLS-AN-190