Effects of low molecular weight heparin and heparin-binding ...
Effects of low molecular weight heparin and heparin-binding ...
Effects of low molecular weight heparin and heparin-binding ...
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ORIGINAL ARTICLE: REPRODUCTIVE BIOLOGYhematoxylin <strong>and</strong> mounted with nertrogum. The number <strong>of</strong>cells per pore was counted in five r<strong>and</strong>omly selected fieldswith a microscope. The cell numbers in each pore wereassessed according to Image-Pro Plus 6.0 (Media Cybernetics).All experiments were performed three times or more with freshcultures.FIGURE 3hCG Secretion TestThe hCG secretion test was designed to explore the effects <strong>of</strong>LMWH <strong>and</strong> HB-EGF on trophoblast differentiation. The humantrophoblast cells (3 10 5 per well) were plated out in24-well plates <strong>and</strong> cultured overnight. The LMWH at differentconcentrations <strong>and</strong> HB-EGF in the presence <strong>of</strong> LMWH wereadded to fresh mediums. After incubated for 48 hours thesupernatant was collected <strong>and</strong> stored at 20 C. The concentration<strong>of</strong> b-hCG in the supernatant was determined using thechemiluminescence method. All experiments were performedthree times or more with fresh cultures.Statistical AnalysisAll values were expressed as the mean SD. Data were analyzedusing one-way analysis <strong>of</strong> variance (ANOVA) with theapplication <strong>of</strong> the Dunnett’s test, the least-significant differencetest, <strong>and</strong> a three-factor ANOVA classification. Differenceswere considered as statistically significant at P.05);within the doses <strong>of</strong> 0.25–2.5 IU/mL, LMWH greatly promotedtrophoblast proliferation <strong>and</strong> enhanced their invasion(P
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