Sanitary requirements for bovine gametes and embryos in ... - CBRA

Ponsart and Pozzi. Sanitary requirements for gametes and embryos.Table 9. Diseases specific OIE recommendations for the importation of bovine embryos (OIE, 2012).Disease* In vivo derived embryos In vitro produced embryos/ovaBovine Brucellosis(BB)*• donor females kept in a country or zone free from BB; or kept in aherd officially free from BB (tests as prescribed in Chapter 1.3);oocytes fertilized with semen meeting the conditions referred toin Chapters 4.5 and 4.6.Blue Tongue Virus(BTV)Foot and MouthDisease (FMD)Bovine Tuberculosis(BT) in cattle orfarmed cervidae*Contagious BovinePleuropneumonia(CBPP)*Lumpy Skin Disease(LSD; caused bygroup III virus, typeNeethling)*• donor cows kept in a BTV free country or in a seasonally freezone or in a vector-protected establishment (in BTV infectedcountries) for at least the 60 days prior to, and at the time of,collection of the embryos;• donor cows subjected to a serological test between 21 and 60days after collection, with negative results;• donor cows subjected to an agent identification test taken on theday of collection, with negative results.FMD free countries• no clinical sign of FMD at the time of collection of the oocytes;• donor kept at the time of collection in a FMD free country or zonewith or without vaccination or a FMD free compartment;• embryos produced in zones with vaccination and destined for anFMD free country or zone without vaccination or an FMD freecompartment: no vaccination of donor and negative results to testsfor antibodies against FMD virus; or vaccinated at least twice (lastvaccination not less than one month and not more than 12 monthsprior to collection); no other animal present in the establishmentvaccinated within the month prior to collection.• no sign of BT during the 24 h prior to embryo collection in the herd of origin; and eitherdonor originated from a herd free from BT (cattle or farmed cervidae) in a country, zone orcompartment free from BT; or kept in a herd free from BT (cattle or farmed cervidae), andsubjected to a tuberculin test for BT with negative results during an isolation period (30days) in the establishment of origin prior to collection (COL).• from CBPP free countries, zones or compartments: donor animals without clinical sign ofCBPP on the day of collection of the embryos/oocytes; kept in a CBPP free country sincebirth or for at least the past 6 months; oocytes fertilized with semen meeting the conditionsof Article 11.8.8;• from CBPP infected countries or zones: no clinical sign of CBPP on the day of COL of theembryos/oocytes; donor subjected to the CFT for CBPP with negative results, on twooccasions (21 to 30 days between each test, 2nd test within 14 days prior to COL); isolatedfrom other domestic bovidae from the day of the first CFT until COL; kept since birth, orfor the past 6 months, in an establishment where no case of CBPP was reported, and thatthe establishment was not situated in a CBPP infected zone; AND EITHER: not beenvaccinated against CBPP; OR vaccinated using a vaccine complying with the standardsdescribed in the TM not more than 4 months prior to COL; oocytes fertilized with semenmeeting the conditions of Article 11.8.9.• from LSD free countries (embryos/oocytes of cattle and water buffaloes): donor animalswithout clinical sign of LSD on the day of COL of the embryos/oocytes;• from countries considered infected with LSD (embryos/oocytes of cattle and waterbuffaloes): no case of LSD has been reported during the 28 days prior to COL in theestablishment; and no clinical sign of LSD on the day of COL; and either: vaccinatedagainst LSD between 28 days and 90 days before first embryo/oocyte COL and thereaftervaccinated annually; or tested with negative serological results (SNT or indirect ELISA)for LSD on the day of embryo/oocyte COL or up to 90 days after last collection; orshowed stable seropositivity on paired samples tested side by side to indirect ELISA orSNT carried out in quarantine (28–60 days apart, one sample on the day of COL).*For each listed disease as well as Enzootic Bovine Leucosis (EBL) and Infectious Bovine Rhinotracheitis/InfectiousPustular Vulvovaginitis (IBR-IPV), Veterinary Authorities of importing countries should require the presentation of aninternational veterinary certificate attesting that the embryos/ova have been collected, processed, and stored in conformitywith the provisions of OIE Chapters 4.7, 4.8, and 4.9.294 Anim. Reprod., v.10, n.3, p.283-296, Jul./Sept. 2013