Semen analysis test - Sri Lanka male female infertility treatment ...

• The biologic significance of this test is unclear and its validity to predictIVF fertilization rate is controversial, and it is equivalent to viabilitystaining.• The HOS test and correlates with semen analysis data but not with thehamster oocyte penetration test.Immunobead testTests for sperm antibodies should be done routinely on all men being evaluatedfor infertility because no semen analysis pattern is characteristic of spermautoimmunity.• The immunobead test (IBT) with beads binding to more than 50 per centof motile sperm is regarded as positive, but there usually is more than 70to 80 per cent IgA binding with clinically significant sperm autoimmunity.Tail-tip only IBT binding is not significant• The indirect IBT in which normal donor sperm are exposed to test serumor seminal plasma can be used to test men with too few motile sperm forthe direct IBT.• An alternative screening method for men with sperm in the semen wouldbe to perform a sperm-mucus penetration test.• The mixed antiglobulin reaction test is an alternative to the IBT• Sperm-mucus penetration tests can be performed by postcoitalexamination of sperm in cervical mucus collected at midcycle or afterestrogen treatment (ethinyl estradiol, 50 mg twice daily for four days) toproduce mucus of equivalent quality.• In vitro capillary mucus penetration (Kremer) tests are particularlyimportant for evaluating the significance of sperm autoantibodies; failure ofsperm to penetrate more than 2 cm in one hour indicates severe spermautoimmunity with a poor prognosis if untreated.