Zoology - University of Gour Banga

P.G. Zoology Syllabus, University of Gour Banga, MaldaZGT 303 (Biophysical Principles and Instrumentations)40 marks1. Principles and uses of analytical instruments: Colorimeter, spectrophotometer, ELISA, flow cytometry andFACS2. Microscopy: Light microscopy, phase-contrast microscopy, fluorescence microscopy, confocal microscopy,SEM and TEM3. Chromatographic techniques: Column chromatography, TLC, GC-MS, HPLC, HPTLC, Ion-exchangechromatography, gel exclusion chromatography and affinity chromatography4. Electrophoresis: PAGE (native and SDS), agarose gel electrophoresis, 2-D gel electrophoresis and isoelectricfocusing5. Centrifugation: Basic principles of sedimentation, differential and density gradient centrifugation6. Crystallography and X-ray diffraction, Basic idea of NMR, Endoscopy, USG, CT scan and MRI7. Polymerase chain reaction: Types and their applications14