Microbiology

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Autumn Meeting 6–9 September 2010 University of Nottingham – www.sgmnottingham2010.org.uk CONTENTS Sessions Hot Topic Lecture 2 Outreach Prize Lecture 2 Peter Wildy Prize for Microbiology Education Lecture 2 NT01 Metals & microbes 3 NT01 Workshop: metals & microbes 10 NT02 Microbial death 13 NT03 Streptococci 16 NT04 Acid stress 22 NT05 Bioremediation of metals 28 NT06 Workshop: prokaryotic taxonomy 30 NT07 New insights into secondary metabolism 32 NT08 Respiratory & septic infections 36 NT09 Extremophiles 42 NT10 Industrial Biotechnology 2025 48 NT11 Bioleaching of metals: new developments in technologies 52 NT12 Protein folding & misfolding 54 NT13 Learning from the evidence: improving microbiology teaching through educational research 58 NT14 Microbiology in the indoor environment 60 NT16 Bacterial vesicles 64 NT17 Microbial models of human disease 65 Sir Howard Dalton Young Microbiologist of the Year Competition 68 Posters NT01 Metals & microbes 71 NT03 Streptococci 84 NT04 Acid stress 92 NT05 Bioremediation of metals 92 NT07 New insights into secondary metabolism 94 NT08 Respiratory & septic infections 96 NT09 Extremophiles 98 NT10 Industrial Biotechnology 2025 101 NT12 Protein folding & misfolding 103 NT14 Microbiology in the indoor environment 104 NT16 Bacterial vesicles 104 NT17 Microbial models of human disease 105 CMM Clinical & medical microbiology 106 ENV Environment 112 FB Fermentation & bioprocessing (industry) 114 SC Systems & cells 115 GM General microbiology 115 Authors 121 Please note: Abstracts are published as received from the authors and are not subject to editing. society for general microbiology sgmconferences
Autumn Meeting 6–9 September 2010 ↑CONTENTS University of Nottingham – www.sgmnottingham2010.org.uk SESSION ABSTRACTS HOT TOPIC & PRIZE LECTURES Hot Topic Lecture ↑CONTENTS Building a cell controlled by a synthetic genome Clyde A. Hutchison III Synthetic Biology Group, J. Craig Venter Institute, Rockville Maryland & San Diego California, USA Starting from a bacterial genome sequence stored as a computer file, we made intentional design changes to the sequence, chemically synthesized it, and brought it to life by transplantation into a recipient cell, to create a new strain of bacterial cells, Mycoplasma mycoides JCVI-syn1.0. These cells are capable of continuous replication, and have expected phenotypic properties. The only genetic information they contain is the synthetic chromosome, including all designed identifying watermark sequences, gene deletions, and polymorphisms. After approximately 20 doublings, every macromolecule in a progeny cell will be a product specified by the synthetic genome. For this reason we like to refer to a cell controlled by a synthetic genome as a ‘synthetic cell’. In the course of this work methods have been developed for the construction, cloning, and installation of large DNA assemblies. It is possible to assemble segments of natural or synthetic DNA up to several hundred kilobases enzymically in vitro. Natural and synthetic DNA greater than 1 Mb can be assembled and cloned by natural recombination in yeast. These methods will facilitate experimental definition of the minimal genetic requirements for cellular life, and should aid in the design of new bacterial strains with useful properties. Outreach Prize Lecture ↑CONTENTS More than a gut feeling Gemma Walton Food & Nutritional Sciences, University of Reading, Earley Gate, Whiteknights Road, Reading RG6 6AP From flatulence to bloating, constipation to diarrhoea, everyone can relate to gut feelings – but how many people know the science behind what is happening? Previous approaches of gut microbiology informing have included flatus collecting cowboys in Colorado and artichoke eating children in Essex. This presentation will look at ways of communicating microbiology to non-scientists, the use of media for getting messages across and also for challenging existing perceptions. Peter Wildy Prize for Microbiology Education Lecture ↑CONTENTS How the mushroom got its spots and other stories Sue Assinder Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA Talking to the public about science was once an activity conducted by a few committed eccentrics under the radar of the normal academic ‘day job’. It is now a legitimate enterprise supported by a framework of national and international science centres and festivals, and is endorsed by funding bodies and university management alike. When I first became involved around 15 years ago, few people outside of a lab had heard of DNA and science communication was working to a ‘deficit model’ (with the unfortunate acronym of ‘PUS’) of increasing public understanding of science by imparting information. Now DNA is part of everyday vocabulary and the focus is on engaging the public as active participants. My enthusiasm for communicating science has taken me from primary schools and draughty village halls to the Royal Show and the House of Lords. It has affected whole aspects of my life – I can no longer cut up an onion without wanting to extract its DNA, nor can I think of body glitter as anything other than a model for the cold virus in mucus. In my talk I will recall the fun I have had as one of the early eccentrics and give a personal perspective on the changing agenda for science communication. Please note: Abstracts are published as received from the authors and are not subject to editing. society for general microbiology 2 sgmconferences
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Microbiology

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