Expression Vectors

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NOTE: Acknowledgment for publications should read "The following reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pcDNA3.1-mAPOBEC3G-V5-6XHis (murine APOBEC3G) from Drs. B. Matija Peterlin and Yong-Hui Zheng." Also include the reference cited above in any publications. ALL RECIPIENTS OF THIS MATERIAL MUST COMPLY WITH ALL APPLICABLE BIOLOGICAL, CHEMICAL, AND/OR RADIOCHEMICAL SAFETY STANDARDS INCLUDING SPECIAL PRACTICES, EQUIPMENT, FACILITIES, AND REGULATIONS. NOT FOR USE IN HUMANS. REV: 11/08/2015 Page 2 of 2
DATA SHEET Reagent: pcDNA3.1-APOBEC3G-V5-6XHis Catalog Number: 10102 Lot Number: 4 040908 Release Category: A Provided: 2.0 µg plasmid DNA in 2.0 µl TE buffer. The plasmid was amplified in HB 101 competent cells (Invitrogen Corp). Description: The cloning insert was obtained from Open Biosystems (Huntsville, AL). Insert for human APOBEC3G was cloned into mammalian expression vector pcDNA3 with a V5 epitope tag from SV5 paramyxovirus and a polyhistidine epitope at the C terminus. Plasmid DNA was transfected into 293T cells and expression of human APOBEC3G was detected with with a monoclonal anti-V5 antibody. <strong>Expression</strong> of human APOBEC3G is driven by the CMV promoter. Genebank Acc# BC024268 Cloning Site: TOPO Cloning Technology was used following manufacture’s instruction (Invitrogen Corp). The size of the insert is 1155 bp. Cloning Vector: The cloning vector is pcDNA3.1D/V5-His-Topo. The size of the cloning vector including the insert is 7069 bp. Recommended Storage: 4°C Contributor: Drs. B. Matija Peterlin and Yong-Hui Zheng References: Yong-Hui Zheng, Dan Irwin, Takeshi Kurosu, Kenzo Tokunaga, Tetsutaro Sata and B. Matija Peterlin. Human APOBEC3F Is Another Host Factor That Blocks Human Immunodeficiency Virus Type 1 Replication. J. Virology 78:6073-6076, 2004. ALL RECIPIENTS OF THIS MATERIAL MUST COMPLY WITH ALL APPLICABLE BIOLOGICAL, CHEMICAL, AND/OR RADIOCHEMICAL SAFETY STANDARDS INCLUDING SPECIAL PRACTICES, EQUIPMENT, FACILITIES, AND REGULATIONS. NOT FOR USE IN HUMANS. REV: 11/08/2015 Page 1 of 2
Page 1 and 2: Expression Vectors
Page 3 and 4: DATA SHEET Reagent: pcDNA3.1-AID-V5
Page 5 and 6: DATA SHEET Reagent: pcDNA3.1-APOBEC
Page 13 and 14: DATA SHEET Reagent: pc-Mu-APOBEC3G-
Page 15: DATA SHEET Reagent: pcDNA3.1-mAPOBE
Page 19 and 20: DATA SHEET Reagent: pcDNA-APO3G Cat
Page 21 and 22: DATA SHEET Reagent: pHIV-APO3G Cata
Page 25 and 26: DATA SHEET Reagent: phAPOBEC3B-HA C
Page 27 and 28: DATA SHEET Reagent: pcDNA3.1 Human
Page 29 and 30: DATA SHEET Reagent: pCMV4-HA-APOBEC
Page 33 and 34: BIV >> ENV
Page 35 and 36: BIV >> GAG
Page 37 and 38: DATA SHEET Reagent: BIV-gag5 Catalo
Page 39 and 40: DATA SHEET Reagent: T4-pMV7 Catalog
Page 41 and 42: DATA SHEET Reagent: T8-pMV7 Catalog
Page 43 and 44: DATA SHEET Reagent: pc.Rh-CD4 Catal
Page 45 and 46: Chemokine Receptors >> CCR2
Page 47 and 48: References: Morgenstern JP, Land H.
Page 51 and 52: DATA SHEET Reagent: pBABE.CCR3 Cata
Page 53 and 54: DATA SHEET Reagent: pcCCR3 Catalog
Page 55 and 56: Chemokine Receptors >> CCR4
Page 61 and 62: DATA SHEET Reagent: pBABE.CCR5 Cata
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DATA SHEET Reagent: pc.Rh-CCR5 Cata
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DATA SHEET Reagent: pBABE.Rh-CCR5 C
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Chemokine Receptors >> CXCR4
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ALL RECIPIENTS OF THIS MATERIAL MUS
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References: Morgenstern JP, Land H.
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References: Morgenstern JP, Land H.
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NOTE: Acknowledgment for publicatio
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DATA SHEET Reagent: pcCCR1 Catalog
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DATA SHEET Reagent: pBABE.CCR1 Cata
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Chemokines >> MIP-1Alpha_MIP-1Beta
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NOTE: The E. coli host strain AD494
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Recipient must not use or incorpora
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DATA SHEET Reagent: pET32a/Rhesus R
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Chemokines >> SDF-1α
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References: Veldkamp, C. T., Peters
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References: Veldkamp, C. T., Peters
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DATA SHEET Reagent: pET14B/Rhesus I
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DATA SHEET Reagent: pcDNA-PtDC-SIGN
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DATA SHEET Reagent: pcDNA3-DC-SIGN
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DATA SHEET Reagent: pMX DC-SIGN Cat
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DC-SIGN-Related >> L-SIGN and SIGNR
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References: Soilleux EJ, Barten R,
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DATA SHEET Reagent: pRS-ERev Catalo
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EIAV >> TAT
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DATA SHEET Reagent: FIV-34F10 DU Ca
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DATA SHEET Reagent: FIV-34F10 DU Ca
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FIV >> GAG
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FIV >> POL
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DATA SHEET Reagent: FIV-34F10 RT Ca
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DATA SHEET Reagent: FIV-34F10 RT Ca
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DATA SHEET Reagent: FIV-PPR Rev Cat
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DATA SHEET Reagent: p96ZM651gp140-C
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DATA SHEET Reagent: p96ZM651gp160-o
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DATA SHEET Reagent: p96ZM651gp140-c
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DATA SHEET Reagent: pSyngp120IgG Ca
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DATA SHEET Reagent: pIIIenv3-1 Cata
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DATA SHEET Reagent: pDOLHIVenvR Cat
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DATA SHEET Reagent: pDOLHIVenv Cata
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DATA SHEET Reagent: HIV-1 clone BaL
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DATA SHEET Reagent: pcDNA 89.6 env
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DATA SHEET Reagent: pDOLHIVenv Cata
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DATA SHEET Reagent: pHXB2-env Catal
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DATA SHEET Reagent: pS15-GFP Catalo
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DATA SHEET Reagent: pSyngp140JR-FL
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DATA SHEET Reagent: pCAGGS SF162 gp
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DATA SHEET Reagent: HIV-1 (YU2) gp1
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DATA SHEET Reagent: pSyngp120JR-FL
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DATA SHEET Reagent: Resurfaced stab
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HIV-1 >> GAG
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Recommended Storage: Keep at -20°C
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Contributor: Marilyn D. Resh, Ph.D.
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Contributor: Marilyn D. Resh, Ph.D.
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References: Sandefur S, Varthakavi
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Scientists at for-profit institutio
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Rochester Medical Center, Tel: 585-
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DATA SHEET Reagent: pNef-ER Catalog
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DATA SHEET Reagent: plconsnefSN Cat
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DATA SHEET Reagent: plconsnefSN Cat
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DATA SHEET Reagent: pcDNA3.1 SF2 Ne
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DATA SHEET Reagent: p96ZM651nef-opt
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DATA SHEET Reagent: pT7consnefhis6
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DATA SHEET Reagent: pcDNA3.1SF2NefF
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HIV-1 >> POL
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References: Bushman FD, Engelman A,
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Contributor: Dr. James M. Groarke,
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Ivanoff LA, Towatari T, Ray J, Kora
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Recommended Storage: -80°C. Contri
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DATA SHEET Reagent: pCV1 Catalog Nu
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DATA SHEET Reagent: pcRev Catalog N
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DATA SHEET Reagent: pCMV-rev (pRev-
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DATA SHEET Reagent: HIV-1 HXB2 Rev
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DATA SHEET Reagent: HIV-1 HXB2 Rev
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HIV-1 >> TAT
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References: Herrmann CH, Rice AP. L
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References: Ott M, Emiliani S, Van
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Recommended Storage: -70°C Contrib
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Contributor: Dr. Udaykumar Ranga Re
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Plasmid Map Recommended Storage: -7
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DATA SHEET Reagent: pcDNA-HVif Cata
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HIV-1 >> VPU
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References: Nguyen, K-L, Llano, M.,
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References: Nguyen, K-L, Llano, M.,
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DATA SHEET Reagent: pEGFP-Vpr Catal
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DATA SHEET Reagent: pGFP-VPR Catalo
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DATA SHEET Reagent: pPA-GFP-VPR Cat
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DATA SHEET Reagent: pCMV-FLAG.DCAF-
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DATA SHEET Reagent: pEGFP-Vpr Catal
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DATA SHEET Reagent: pMM310 Catalog
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HIV-2 >> Tat
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pGal4Tat2 (K70A) 3639 1 7/15/97 Sub
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pGal4Tat2 (K70A) 3639 1 7/15/97 Sub
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pGal4Tat2 (K70A) 3639 1 7/15/97 Sub
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pGal4Tat2 (K70A) 3639 1 7/15/97 Sub
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pGal4Tat2 (K70A) 3639 1 7/15/97 Sub
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pGal4Tat2 (K70A) 3639 1 7/15/97 Sub
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1. Transfer the beads into a 15 ml
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DATA SHEET Reagent: pBC12 (CMV) IL-
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DATA SHEET Reagent: pLiv7 Catalog N
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DATA SHEET Reagent: pFT-1-LEDGF Cat
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DATA SHEET Reagent: CMVR VRC03 H Ca
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DATA SHEET Reagent: CMVR VRC03 L Ca
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DATA SHEET Reagent: 10E8 mAb Heavy
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DATA SHEET Reagent: 10E8 mAb Light
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DATA SHEET Reagent: 7H6 mAb Light c
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DATA SHEET Reagent: 35O22 mAb Heavy
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DATA SHEET Reagent: 35O22 mAb Light
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SIV >> Tat
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DATA SHEET Reagent: SIV pGEX-KG vpr
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TRIM5α >> All
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Contributor: Drs. Joseph Sodroski a
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References: Stremlau, M et al. The
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References: Stremlau, M et al. The
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Natl. Acad. Sci. USA, 2002, 99 (2);
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DATA SHEET Reagent: 8400-E03 (eSD-H
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DATA SHEET Reagent: 8400-E11 (tev-M
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DATA SHEET Reagent: 8400-E12 (tev-p
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DATA SHEET Reagent: 8400-E14 (tev-N
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DATA SHEET Reagent: 8400-E15 (tev-P
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DATA SHEET Reagent: 8400-E16 (tev-R
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DATA SHEET Reagent: 8400-E17 (tev-I
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DATA SHEET Reagent: 8400-E18 (tev-S
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DATA SHEET Reagent: 8400-E19 (tev-T
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DATA SHEET Reagent: 8400-E20 (tev-E
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DATA SHEET Reagent: 8400-E22 (tev-p
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DATA SHEET Reagent: 8400-E23 (tev-C
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DATA SHEET Reagent: 8400-E24 (tev-N
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DATA SHEET Reagent: 8400-E25 (tev-P
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DATA SHEET Reagent: 8400-E26 (tev-R
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DATA SHEET Reagent: 8400-E27 (tev-I
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DATA SHEET Reagent: 8400-E28 (tev-S
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DATA SHEET Reagent: 8400-E29 (tev-T
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DATA SHEET Reagent: 8400-E30 (tev-E
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DATA SHEET Reagent: 8400-E13 tev-CA
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DATA SHEET Reagent: 8400-E21 (tev-M
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DATA SHEET Reagent: 8400-E31 (SU-Hi
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DATA SHEET Reagent: 8400-E33 (Env-H
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XMRV >> Expression Vectors
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Expression Vectors

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