Developing rice restorer lines resistant to bacterial blight, blast and brown plant hopper by molecular pyramiding
Bacterial blight, blast and brown plant hopper resistance (BB, BL and BPH respectively) genes Xa23, Pi-9, Bph14 and Bph15 were pyramided into an elite restorer line R1005 by using marker assisted selection backcrossing (MABC) and simple sequence repeats (SSR). PCR markers facilitated and accelerated the process of pyramiding of Xa23, Pi-9, Bph14 and Bph15 genes. In this study 75-1-127 harboring Pi9, JYQ9008 harboring Xa23, and B5 harboring Bph14 and Bph15 against resistant bacterial blight, blast and brown planthopper were used as R-genes donor respectively. The pyramided genes and evaluation of agronomic traits related to bacterial blight, blast and brown plant hopper represent best ways in which resistance can be studied. Largest resistance levels were observed against the bacterial blight followed by blast and lastly brown plant hopper pyramid lines. PCR markers for these four genes, Xa23, Pi-9, Bph14, 15 were made available. DNA marker technology was used to identify plants that contained resistant genes to BB, BL and BPH. Restorer lines are known to determine success of pyramided genes. Hence careful background searches should be done before settling on one. Resistant lines can really be the answer to environmental problems that hamper growth of food crops particularly rice and ensure food security for the vulnerable people in society. Pyramiding is considered an ethical way of creating better varieties. The newly variety we created that was resistant to all the three factors had a higher yield than the control. These traits were carried through successive generations as was the case in the hybrid variety. More articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Bacterial blight, blast and brown plant hopper resistance (BB, BL and BPH respectively) genes Xa23, Pi-9, Bph14 and Bph15 were pyramided into an elite restorer line R1005 by using marker assisted selection backcrossing (MABC) and simple sequence repeats (SSR). PCR markers facilitated and accelerated the process of pyramiding of Xa23, Pi-9, Bph14 and Bph15 genes. In this study 75-1-127 harboring Pi9, JYQ9008 harboring Xa23, and B5 harboring Bph14 and Bph15 against resistant bacterial blight, blast and brown planthopper were used as R-genes donor respectively. The pyramided genes and evaluation of agronomic traits related to bacterial blight, blast and brown plant hopper represent best ways in which resistance can be studied. Largest resistance levels were observed against the bacterial blight followed by blast and lastly brown plant hopper pyramid lines. PCR markers for these four genes, Xa23, Pi-9, Bph14, 15 were made available. DNA marker technology was used to identify
plants that contained resistant genes to BB, BL and BPH. Restorer lines are known to determine success of pyramided genes. Hence careful background searches should be done before settling on one. Resistant lines can really be the answer to environmental problems that hamper growth of food crops particularly rice and ensure food security for the vulnerable people in society. Pyramiding is considered an ethical way of creating better varieties. The newly variety we created that was resistant to all the three factors had a higher yield than the control. These traits were carried through successive generations as was the case in the hybrid variety. More articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Int. J. Agri. & Agri. R.<br />
Combination name<br />
HD(<br />
d)<br />
PH<br />
(cm)<br />
P/P<br />
PL<br />
(cm)<br />
S/P<br />
FG/P<br />
GD/<br />
(10cm)<br />
SSR (%)<br />
1000G<br />
-W(g)<br />
扬 两 优 6 号 ( CK3) 90 130.9 8.8 27.1 178.0 142.9 65.6 79.98 27.24 34.04<br />
Hua1165S/MD12086-41 81 120.5 11.3 28.0 148.8 123.1 53.2 82.76 27.33 39.83<br />
Hua 1165S/MD12086-293 81 118.4 9.7 28.7 158.6 132.5 55.2 83.33 26.42 36.84<br />
Hua 1165S/MD12086-352 81 118.9 11.8 25.5 143.2 110.1 60.6 77.20 26.77 37.15<br />
Hua 1165S/MD12086-1089 82 120.3 10.7 27.7 146.9 120.2 52.9 81.96 26.40 38.49<br />
Hua 1165S/MD12086-1351 81 117.0 10.7 27.1 151.0 121.9 55.8 80.31 26.73 37.11<br />
Hua 1165S/R1005 83 118.1 9.8 28.0 160.0 132.2 57.2 82.68 28.59 41.22<br />
Hua 1165S/B5 82 115.0 14.7 27.3 136.1 104.4 49.9 76.85 25.71 36.82<br />
Hua 1165S/75-1-127 80 111.7 13.2 27.2 139.4 110.5 51.1 79.07 26.10 34.96<br />
Hua 1165S/CBB23 81 112.3 12.8 26.3 146.9 123.5 55.8 84.38 26.12 34.87<br />
Rong feng A/MD12086-394 83 118.8 9.8 25.7 170.9 142.1 66.5 83.30 27.23 41.06<br />
Rong feng A/MD12086-1351 82 116.6 11.8 25.7 161.8 137.9 62.6 84.71 27.50 41.98<br />
Rong feng A/R1005 83 115.5 9.5 25.0 176.7 144.4 70.8 81.61 28.10 38.11<br />
Y/P<br />
(g)<br />
Table 8. Hybrid combinations of <strong>rice</strong> quality performance.<br />
Combination name<br />
BRR<br />
( %)<br />
BRR<br />
( %)<br />
MRR<br />
(%)<br />
ChGR<br />
(%)<br />
Ch<br />
(%)<br />
GL<br />
(mm)<br />
A R<br />
AC<br />
( %)<br />
GC<br />
(mm)<br />
GTL<br />
Rong feng A/MD12086-41 79.0 65.10 43.70 87.0 51.4 6.1 2.9 20.51 45.3 2.7<br />
Rong fengA/MD12086-293 77.9 65.35 44.04 77.0 36.1 6.1 2.9 20.94 51.5 2.8<br />
Rong fengA/MD12086-352 78.1 68.27 64.36 47.0 20.9 6.2 3 12.77 77.5 2.7<br />
Rong fengA/MD12086-394 85.0 65.06 33.72 93.0 46.9 6 2.8 19.86 65.0 3.2<br />
Rong fengA/MD12086-1089 79.4 66.05 53.37 76.0 38.2 6.2 2.9 19.95 52.0 2.3<br />
Rong fengA/MD12086-1351 78.7 67.76 43.15 76.5 33.3 6.4 2.9 20.50 51.5 2.9<br />
Q2A/MD12086-41 78.6 69.08 65.89 96.5 65.1 6.2 2.9 14.72 77.0 3.2<br />
Q2A/MD12086-352 78.1 68.27 64.36 47.0 20.9 6.2 3 12.77 77.5 2.7<br />
Q2A/MD12086-1089 78.5 66.62 56.75 opaque opaque 6.3 3 13.40 74.5 3.2<br />
Q2A/R1005 78.2 67.40 57.23 69.0 14.9 6.3 3 13.40 76.0 3.1<br />
Q 优 6 号 ( CK2) 78.6 67.57 61.25 40.0 18.7 6.2 3 13.93 76.5 3.0<br />
Hua1165S/MD12086-41 79.3 68.69 59.63 opaque opaque 6.5 3.1 13.48 71.0 2.9<br />
Hua1165S/MD12086-1089 76.3 65.52 56.84 opaque opaque 6.4 3.1 11.50 64.5 2.8<br />
Hua1165S/R1005 77.6 68.49 63.93 14.0 2.8 6.6 3.2 13.07 71.3 2.9<br />
BRR=Brown <strong>rice</strong> rate, RR= Rice rate, MRR= Milled <strong>rice</strong> rate, ChGR= Chalky grain rate, CH= Chalkiness, GL=<br />
Grain length, AR= Aspect Ratio, AC= Amylose content, GC= Gel Consistency, GTL= Gelatinization temperature<br />
level.<br />
Discussion<br />
In this study, the markers assisted <strong>pyramiding</strong> of<br />
Xa23, Pi-9, Bph14 <strong>and</strong> Bph15 resistances genes in<strong>to</strong><br />
R1005 <strong>and</strong> its hybrids has facilitated the development<br />
of resistance against pathogens <strong>and</strong> pests in <strong>rice</strong>.<br />
Pyramiding of several resistance genes is an<br />
important <strong>and</strong> effective method that can protect <strong>rice</strong><br />
<strong>plant</strong>s from diseases <strong>and</strong> the environment of <strong>to</strong>xic<br />
effects arising from usage of pesticides. We<br />
introgressed four dominant <strong>resistant</strong> genes Xa23, Pi-<br />
Mamadou et al.<br />
9, Bph14 <strong>and</strong> Bph15 in<strong>to</strong> an elite <strong>res<strong>to</strong>rer</strong> line. Most<br />
studies reported an additive effect <strong>and</strong> epistasis <strong>by</strong><br />
<strong>pyramiding</strong> two genes or more genes controlling <strong>plant</strong><br />
resistance <strong>to</strong> pathogen or insects. Pyramiding two<br />
dominant <strong>bacterial</strong> <strong>blight</strong> resistance genes, Xa7 <strong>and</strong><br />
Xa21, in<strong>to</strong> Minghui 63 <strong>and</strong> its hybrids showed a<br />
significant additive effect of two genes (Zhu et al.,<br />
2004 Jie et al., 2012). The identification of BACs<br />
carrying the most closely linked markers is a crucial<br />
step <strong>to</strong>wards the cloning of a gene (Haiyuan et al.,<br />
Page 163
Change language