Stemgent mRNA Reprogramming System - Miltenyi Biotec

Stemgent mRNA Reprogramming System
The Stemgent mRNA Reprogramming System enables
non-viral, non-integrating, clinically-relevant reprogramming
of human cells. Messenger RNA-based reprogramming
completely eliminates the risk of genomic integration and
mutagenesis inherent to DNA and viral-based technologies
while offering a highly robust and efficient reprogramming
system. Stemgent’s mRNA Reprogramming System and
validated protocol combines everything you need for
successful reprogramming of your cells.
• Non-integrative technology eliminates risk
of insertional mutagenesis
• Transient protein expression levels are easily regulated
• Faster reprogramming, colonies appear within 16 days
• Proven reprogramming protocol using normal and
diseased cells
• Suitable for clinical applications; disease modeling,
drug discovery, and regenerative medicine
Antibody, +DAPI
Oct4
Klf4
Sox2 c-Myc
Lin-28 nGFP
Stemgent mRNA Reprogramming
Factors Set: hOKSML
The hOKSML Set consists of in vitro transcribed mRNA
synthetically modified to minimize innate antiviral
responses to single stranded RNA. mRNA does not integrate
into the host cell DNA and therefore allows for transient
re-programming factor expression required to reprogram
cells.
Stemgent Pluriton
mRNA Reprogramming Medium
The Pluriton Medium was designed specifically to support
mRNA based reprogramming. Pluriton Medium is an
essential component of the mRNA Reprogramming System.
Each lot is qualified for mRNA-based reprogramming of
human fibroblasts.
Stemgent mRNA Reprogramming –
Qualified Kit
The mRNA Reprogramming-Qualified Kit includes the
Stemgent mRNA Reprogramming Factor Set: hOKSML,
Pluriton mRNA Reprogramming Medium, and B18R, a
critical component required to inhibit interferon alpha
(IFN-α). All components in the kit have been tested together
to successfully reprogram BJ fibroblasts.
Figure 1: Expression of mRNA reprogramming factors in BJ
fibroblasts. BJ fibroblasts were transfected with Stemgent mRNA
encoding Oct4, Klf4, Sox2, c-Myc, Lin-28, and nGFP as a control. Protein
expression and localization were analyzed by immunocytochemistry
of Oct4, Klf4, Sox2, c-Myc (all shown in red) and Lin-28, nGFP (shown in
green). All images show nuclear counterstain using DAPI (blue).