Cleaver Scientific Produktkatalog

More documents

Recommendations

Info

eagents & CHEMICALS PAGE and Blotting Buffers Buffers are available in powder sachets for a range of native and denaturing protein gel electrophoresis techniques and Western blotting. Each powder sachet may be reconstituted to make 1 litre of 10X stock solution. Running buffers are also available in 1 litre and 5 litre volumes as ready made 10X stock solutions. KEY FEATURES • Convenient, pre-made stock solution or powder – just dilute or dissolve as necessary with water • Save time & trouble – no weighing, pH adjustment or need to stock individual compounds • Long shelf-life • Consistency assured – rigorous QC for reproducible separations TECHNICAL SPECIFICATIONS Powder Buffer Tris-Glycine SDS Tris-Glycine (Towbin Buffer) Composition Each litre of 1x working solution contains: Tris-base (25mM); glycine (192mM); SDS, 0.1% (w/v); followed by distilled water. Working solution pH = 8.3. Each litre of 1x working solution contains: Tris-base (25mM); glycine (192mM); followed by distilled water. Working solution pH = 8.3. Applications Denaturing SDS-PAGE for most cellular proteins, 10- 200kDa in size Native PAGE or Transfer buffer (with addition of methanol- sup- BP Grade ultra pure water BP Grade Sterile Water has endotoxins removed by electrostatic filtration at the final purification stage prior to autoclaving. The LAL tested water conforms to the standard having less than 0 WFi compatible. Ponceau S Ponceau S staining solution is reusable and available in a convenient 500ml volume for membrane staining and early protein detection following transfer before western blotting. Ponceau S may also be supplied a powder staining kit for long-term storage. ORDERING INFORMATION POWDER BUFFERS CSL-TGSDSP CSL-TGP Powdered Tris-Glycine-SDS Running buffer 10X stock Powdered Tris-Glycine buffer 10X stock LIQUID BUFFERS TG10X1L TG10X5L TG-SDS10X1L TG-SDS10X5L Buffer Tris-Glycine 10 x 1 litre Buffer Tris-Glycine 10 x 5 litre Buffer Tris-Glycine SDS 10 x 1 litre Buffer Tris-Glycine SDS 10 x 5 litre CSL-PSS Ponceau S staining solution (500ml) CSL-PSB Ponceau S staining solution powder staining kit (makes 2000ml) UPW1000 BP Grade Sterile Water, 1000ml RFW250 RNase-Free Water, 1x250ml RFW50X5 RNase-Free Water, 50x5ml
eagents & CHEMICALS Protein Markers Stable for up to 2 years if stored at -20°C and supplied pre-stained in gel loading buffer for direct loading, <strong>Cleaver</strong> <strong>Scientific</strong> PINK Plus and BLUE Wide Range recombinant protein markers are perfect for SDS-PAGE applications. 47 Sizes range from 10-175kDa for PINK Plus and 10-245kDa for BLUE Wide Range, making both markers suitable for accurate molecular weight determination of most cellular proteins. Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly interspersed bands of uniform intensity. Coloured reference bands serve as visual indicators of electrophoresis run progression and the efficiency of western transfer onto membranes following SDS-PAGE. Both PINK Plus and BLUE Wide Range markers can be detected at volumes as low as 2.5μl per well. PPL Pink Plus TECHNICAL SPECIFICATIONS Cat. No. CSL-PPL CSL-BBL Size Range 10-175kDa 10-245kDa Number of Bands 11 12 Reference Bands 10, 40 and 90kDa; blue 25 and 75kDa; green & red Contents maximum 2.2mg total protein in 15% (v/v) glycerol, 2% SDS maximum 2.4mg total protein in 15% (v/v) glycerol, 2% SDS Volume Supplied 500μl 500μl Storage 3 months at 4°C & 24 months at -20°C Loading Volume 2.5-5μl/well Number of Applications 100-200 Source recombinant proteins, various sources BBL Blue Wide Range Blotting membranes Used in Western blotting, Slot and Dot blotting, Southern and Northern blotting. PVDF with nitrocellulose (proteins) and nylon (RNA and DNA) membranes are available for different application needs and in different formats. Membranes are supplied in sheet form and as a 3m. roll which can be cut to size to fit experimental needs. These membranes are suitable for transfer of proteins and nucleic acids from polyacrylamide and agarose gels. Offered in 0.2μm and 0.45μm. Blot absorbent filter paper This blot-absorbent filter paper is supplied in packs of 50 and in sizes of 10x10cm and 20x20cm. Its 1mm thick texture and high buffer retention properties, being able to absorb twice its own weight in buffer, allow it to exert the gel-membrane compression needed for efficient transfers. ORDERING INFORMATION PROTEIN MARKERS CSL-PPL Pink Plus Prestained Protein Ladder, 10-175kDa, with 10, 40 & 90kDa reference bands, 1x 500μl vial. BLOTTING MEMBRANES AND ROLLS CSL-RNC45 Nitrocellulose roll, 0.3x3m (w x l), 0.45μm CSL-RNC2 Nitrocellulose roll, 0.3x3m (w x l), 0.2μm PVDF0.2S PVDF0.45S 25 Pre-cut PVDF 20 x 20 cm 0.2 μm 25 Pre-cut PVDF 20 x 20 cm 0.45μm CSL-BBL Blue Wide Range Prestained Protein Ladder, 10-245kDa, with 25 & 75kDa reference bands, 1x 500μl vial. CSL-RNY45 Positively charged supported nylon, 0.24x3m (w x l) CSL-RNY2 Positively charged supported nylon, 0.24x3m (w x l) PVDF0.2R PVDF0.45R Roll PVDF 24 cm x 3 m, 0.2μm Roll PVDF 24 cm x 3m, 0.45μm BLOT ABSORBENT FILTER PAPER CSL-BP1010 Blot-Absorbent Filter paper, 10x10cm, pack of 50 CSL-BP2020 Blot-Absorbent Filter paper, 20x20cm, pack of 50 www.cleaverscientific.com T_+44 (0)1788 565 300 E_info@cleaverscientific.com
Page 1 and 2: Electrophoresis solutions for the L
Page 3 and 4: 3 A complete range of Gel Electroph
Page 5 and 6: 5 Ergonomically designed OMNIPETTE
Page 7 and 8: 7 Horizontal gel systems Wide range
Page 9 and 10: 9 MSMAXI MSSCREEN miniRAPIDE MSMIDI
Page 11 and 12: gel trays 11 Gel trays are UV trans
Page 13 and 14: Midi 13 With gel tray options of 10
Page 15 and 16: Maxi 15 multiSUB Maxi is primarily
Page 17 and 18: Accessories 17 The multiSUB Series
Page 19 and 20: miniONE 19 The multiSUB miniONE el
Page 21 and 22: miniRAPIDE 21 The miniRAPIDE makes
Page 23 and 24: 23 runVIEW is an innovative system
Page 25 and 26: 25 blue light source features a sli
Page 27 and 28: eagents & CHEMICALS 27 dnaLADD RS P
Page 29 and 30: eagents & CHEMICALS agarOS Cleaver
Page 31 and 32: 31 Vertical gel systems 3 tank size
Page 33 and 34: 33 VS20WAVE MAXI SYSTEM VS30 MAXI-P
Page 35 and 36: 35 simple, rapid, leak-proof gel ca
Page 37 and 38: Mini Wide Systems The Mini Wide ver
Page 39 and 40: 39 Gel Casting Dual purpose PAGE in
Page 41 and 42: Comb options 41 omniPAGE Vertical G
Page 43 and 44: Maxi Plus 43 The Maxi Plus unit pro
Page 45: 45 Semi Dry Blotters These Semi Dry
Page 49 and 50: eagents & CHEMICALS ECL Substrates
Page 51 and 52: horizontal system packages 51 MSMIN
Page 53 and 54: C llas Clinical Cellulose acetate e
Page 55 and 56: 55 APPS22 APPS4SP APPSM8 Cellas Box
Page 57 and 58: Each unit contains ultra-soft silic
Page 59 and 60: 59 DENATURING GRADIENT Innovative C
Page 61 and 62: POW Rsupplies 61 Whether you requir
Page 63 and 64: Power Supply SELECTION GUIDE 63 Hor
Page 65 and 66: 65 gelPRO chemLITE chemiPRO chemiPR
Page 67 and 68: h i O omniDOC The omniDOC systems o
Page 69 and 70: gel gelL ONI T 69 Low cost, all-in-
Page 71 and 72: chem i LIT 71 chemiLITE is an optim
Page 73 and 74: chem i PRO XS 6/9 73 ChemiPRO XS of
Page 75 and 76: gel analysis 75 CLIQS gel analysis
Page 77 and 78: proBLUEVIEW Dual colour transillumi
Page 79 and 80: Gel Drying 79 With a drying area of
Page 81 and 82: FINGER-HOOK TAKES THE WEIGHT, FOR A
Page 83 and 84: vortex mixer This variable speed Vo
Page 85 and 86: heaters and stirrers With a durable
Page 87 and 88: microBLOCK A breakthrough in size a
Page 89 and 90: Glove Boxes Available in four sizes
Page 91 and 92: UV STERILISATION PCR cabinets These
Page 93 and 94: identitapes Self-adhesive IdentiTap
Page 95 and 96: 95 AND WASTE BINS STORAGE BOXES, RA

Cleaver Scientific Produktkatalog

Create successful ePaper yourself

Delete template?

Save as template?