Occurrence and diversity of microorganisms isolated from selected solid waste dumpsites in parts of Ondo State, Nigeria| IJMM-Vol-13-No-1
This study was designed to assess the microbiological and physicochemical properties of soil samples from selected waste dumpsites in parts of Ondo State, Nigeria. The findings of the microbiological analysis carried out showed the mean values of the total heterotrophic bacterial counts ranged from 1.17 × 106 ± 0.08 cfu/g – 7.67 × 106 ± 0.01 cfu/gwhile the total fungal counts recorded ranged from 1.00 × 104 ± 0.02 sfu/g to 6.33 × 104 ± 0.11 sfu/g. The recorded physicochemical properties of the soil samples ranged from 5.4 to 7.9, 4.95 to 45.36%, 0.86 to 2.50% and 2.38 to 11.35% for pH, organic matter, organic nitrogen and organic carbon respectively. The soil particles of the selected dumpsites recorded a range of sand between 55 – 80%, silt 7 – 31% and clay 4 – 29%. The microbial isolates characterized and identified include Bacillus, Alcaligenes, Staphylococcus, Proteus, Micrococcus, Pseudomonas, Serratia, Ochrobacterium, Escherichia and Aspergillus, Penicillium, Mucor, Rhizopus, Cladiosporiumand Trichodermarespectively. Bacillus and Aspergillus species were the most prevalent microorganisms isolated from the selected dumpsites and studies have revealed these organisms to be pathogenic in nature, which could pose serious health risk to residents in and around the dumpsites.
This study was designed to assess the microbiological and physicochemical properties of soil samples from selected waste dumpsites in parts of Ondo State, Nigeria. The findings of the microbiological analysis carried out showed the mean values of the total heterotrophic bacterial counts ranged from 1.17 × 106 ± 0.08 cfu/g – 7.67 × 106 ± 0.01 cfu/gwhile the total fungal counts recorded ranged from 1.00 × 104 ± 0.02 sfu/g to 6.33 × 104 ± 0.11 sfu/g. The recorded physicochemical properties of the soil samples ranged from 5.4 to 7.9, 4.95 to 45.36%, 0.86 to 2.50% and 2.38 to 11.35% for pH, organic matter, organic nitrogen and organic carbon respectively. The soil particles of the selected dumpsites recorded a range of sand between 55 – 80%, silt 7 – 31% and clay 4 – 29%. The microbial isolates characterized and identified include Bacillus, Alcaligenes, Staphylococcus, Proteus, Micrococcus, Pseudomonas, Serratia, Ochrobacterium, Escherichia and Aspergillus, Penicillium, Mucor, Rhizopus, Cladiosporiumand Trichodermarespectively. Bacillus and Aspergillus species were the most prevalent microorganisms isolated from the selected dumpsites and studies have revealed these organisms to be pathogenic in nature, which could pose serious health risk to residents in and around the dumpsites.
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Table 3.The physicochemical parameters of the selected dumpsites in Akure town (± standard error)
Parameters A B C D E
pH
Cs
6.00±0.01
4.11±0.01
6.20±0.10
5.00±0.01
6.87±0.01
5.42±0.30
7.15±0.03
4.99±0.02
6.54±0.10
5.31±0.10
Conductivity (ohms)
Cs
33.15±0.01
42.02±0.21
20.01±0.01
22.01±0.17
15.08±0.45
15.76±0.12
20.71±0.13
17.32±0.12
28.04±0.05
19.52±0.57
Organic Matter %
Cs
12.76±0.03
4.91±0.01
23.16±0.01
10.01±0.01
29.10±0.03
9.33±0.02
28.21±0.01
11.00±0.01
45.36±0.28
10.44±0.02
Organic Nitrogen (%)
Cs
0.97±0.01
1.11±0.05
0.91±0.00
0.90±0.03
0.91±0.43
1.39±0.01
0.86±0.58
1.71±0.04
1.00±0.76
0.73±0.02
Organic Carbon (%)
Cs
2.38±0.33
6.35±0.02
7.36±0.09
6.00±0.01
10.65±0.01
6.67±0.01
11.26±0.01
5.87±0.01
11.35±0.18
6.11±0.05
Sand (%)
Cs
67.68±0.33
75.81±0.17
60.68±1.66
63.20±0.22
67.90±0.11
77.48±0.07
58.12±0.03
60.45±0.14
62.36±1.76
73.51±0.01
Silt (%)
17.84±0.08 15.59±1.87 5.77±0.13 25.59±0.08 8.46±0.09
Cs
19.42±0.01 15.00±0.41 7.99±0.03 24.55±0.05 15.62±0.01
Clay (%)
14.48±0.41 23.73±0.03 18.23±0.05 16.29±1.65 29.18±1.65
Cs
4.77±0.11 21.80±0.26 14.53±0.51 15.00.±0.03 10.87±0.71
KEY: Cs = Control Soil. Each value represents a mean of two readings. A, B, C, D and E = Dumpsites
Table 4.The physicochemical parameters of selected dumpsites in Ondo town (± standard error)
Parameters A B C D D
pH
Cs
5.875±0.00
4.398±0.01
6.325±0.07
3.12±0.03
6.921±0.03
4.01±0.15
Conductivity (ohms) 4.20±0.07 29.09±0.07 24.19±0.04
Cs
3.69±0.31 6.08±0.57 3.88±0.02
Organic Matter %
4.95±0.04 14.56±0.13 18.54±0.57
Cs
8.14±0.03 4.17±0.29 4.32±0.01
Organic Nitrogen (%) 1.01±0.00 1.38±0.04 2.50±0.03
Cs
1.00±0.76 1.50±0.10 0.96±0.01
Organic Carbon (%) 2.86±0.02 8.26±0.02 2.67±0.43
Cs
7.35±0.18 7.00±0.39 5.23±0.06
Sand (%)
65.68±0.01 79.68±0.44 80.41±1.20
Cs
67.68±0.06 65.11±0.01 65.29±0.04
Silt (%)
20.84±0.03 16.48±0.77 7.00±0.67
Cs
11.64±0.01 11.84±0.01 9.71±0.03
Clay (%)
13.48±1.38 3.84±1.57 12.59±1.52
Cs
20.68±0.06 23.05±0.54 18.89±0.02
KEY: Cs = Control Soil. Each value represents a mean of two readings.
6.108±0.19
4.58±0.11
20.56±0.67
4.10±0.51
18.00±0.31
4.77±0.71
2.02±0.01
1.09±0.08
6.66±0.09
7.18±0.01
55.09±1.55
67.00±0.01
31.44±0.03
10.29±0.01
13.47±0.08
22.71±0.06
6.773±0.01
3.81±0.01
41.88±0.09
4.12±0.01
17.20±0.02
4.00±0.01
1.11±0.04
0.970±0.02
3.01±0.04
7.55±0.49
60.26±0.88
55.37±0.03
28.11±1.66
32.40±0.03
11.63±1.11
12.23±0.06
A poorly sorted nature of particle sizes may
indicate soil not formed from natural process of
weathering of the underlying parent material but
rather, from the deposited wastes (Okoronkwoet
al., 2006).
5
Olawaleet al.
Consequently, the frequency of occurrence of the
microbial isolates can be influenced by the soil
particle content which has been altered by the
waste being disposed at the dumpsites.