Working with Ciprofloxacin HCl - Cellgro

Working with Ciprofloxacin HCl
Mediatech, Inc. 9345 Discovery Blvd. Manassas, VA 20109 800-CELLGRO (235-5476) www.cellgro.com
cellgro®
by Mediatech, Inc.
Ciprofloxacin HCl powder should be stored at room temperature below 30°C. Liquid stock solutions should be stored frozen
(-20°C).
To prepare a stock solution add the appropriate amount of powder to distilled deionized water while mixing.
NOTE: Adding water directly to the powder may result in insoluble mixtures that cannot be filtered without losing potency.
After ensuring that the mixture is completely solubilized, filter sterilized. Store at -20°C. If desired, the pH may be adjusted.
Media containing freshly added Ciprofloxacin HCl, at an effective working concentration of 5-25 μg.mL (most commonly 10
μg/mL), should be added to cells seeded at a density of 103 to 105.
Cells should be re-seeded or sub-cultured with media containing Ciprofloxacin HCl at 3 to 4 day intervals.
Cells should be treated for 12-20 days, after which, it is no longer necesary to include Ciprofloxacin HCl in the media.
Ciprofloxacin HCl may be used in combination with other antibiotics.
Ciprofloxacin HCl is soluble in water. Stock concentrations of up to 10 mg/mL frozen can be safely recommended as far as long
term storage of liquid solution.
Product Catalog No. Size
Ciprofloxacin HCl, Powder 61-277-RF
61-277-RG
1 x 1 g
1 x 5 g