Worm Breeder's Gazette 17†4‡ - WormBook
Worm Breeder's Gazette 17†4‡ - WormBook
Worm Breeder's Gazette 17†4‡ - WormBook
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An attempt to slow aging in C. elegans. 29. No positive effect of sodium<br />
salicylate<br />
Vladimir V. Bakaev 1,2 , Lyudmila M. Bakaeva 1<br />
1Institute of Internal Medicine, 2a, Vladimirovskii spusk, Novosibirsk, 630003, Russia<br />
2bakaev@online.nsk.su<br />
The purpose of this study was to investigate the effect of sodium salicylate in water solutions<br />
on the nematode life span. In this experiment sodium salicylate was used in following dilutions:<br />
1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre<br />
wells containing 0.5 ml of liquid medium (with E. coli and without sodium salicylate) during 4<br />
hours, then they were discarded and newborn larvae were transferred in next wells (without<br />
sodium salicylate in medium) every day (one worm in one well) beginning from third day. Then,<br />
beginning from 3 rd day, these worms were transferred every day in next wells containing<br />
medium with sodium salicylate in any concentration. This investigation was carried out in<br />
temperature +21°C and in the darkness. The obtained results are presented in the following<br />
table.<br />
Concentration of sodium salicylate (mg/L) n Longevity (days)<br />
Mean±S.E.Maximal<br />
Control 1210.67±1.82 22<br />
10 000 12 toxic<br />
1 000 1211.08±0.65 16<br />
100 1210.75±1.40 22<br />
10 1210.08±0.82 16<br />
1 1210.75±1.15 19<br />
0.1 12 9.33±0.54 12<br />
Conclusion: If sodium salicylate solution was applied to C. elegans, it was not able to increase<br />
their mean longevity in comparison with control.<br />
Acknowledgment: The authors wish to express their thanks to CGC for providing C. elegans<br />
(Bristol, N2) and E. coli OP50.
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