Mechanical strain regulation of the Chicken glypican-4 gene ...

More documents

Recommendations

Info

ABSTRACT Comparison of RNA fingerprinting of the avian eggshell gland (ESG) without and with an egg revealed up-regulation of a 382-bp cDNA fragment that showed high homology to the mammalian glypican 4 (GPC-4). The gene sequence revealed a conserved glypican signature, a GPI-anchorage site and cystein residues most of which were conserved. GPC-4 was expressed in the ESG in a circadian fashion only during the period of eggshell calcification, when maximal mechanical strain was imposed. Removal of the egg just before to its entry into the ESG, with consequent elimination of the mechanical strain caused reduction in the gene expression. Artificial application of the mechanical strain induced expression of the GPC-4 gene that was related to the level of the strain. GPC-4 expression was strain-dependent in other parts of the oviduct. In the ESG, GPC-4 was expressed exclusively by the glandular epithelium and not by the pseudostratified epithelium facing the lumen. In summary, we cloned the avian homologue of GPC-4, established its pattern of expression in the avian ESG and demonstrated for the first time that this gene is regulated by mechanical strain. Key words: Heparan sulfate proteoglycans; calcification, eggshell gland 2
Abbreviations ESG - avian eggshell gland GPC-4 - glypican 4 OPN- osteopontin ECM - extracellular matrix GPI- glycosyl phosphatidyl inositol RACE- Rapid amplification of cDNA ends SSRE- shear stress response element PE- pseudostratified epithelium facing the lumen GE- inner glandular epithelium 3
Page 1: AJP-Regu Articles in PresS. Publish
Page 5 and 6: about 10% of the total body calcium
Page 7 and 8: for 1 min, 60°C for 1 min and 68°
Page 9 and 10: RESULTS Identification of the chick
Page 11 and 12: The expression of the GPC-4 gene in
Page 13 and 14: to become part of the assembly of p
Page 15 and 16: 9. Clark EA, King WG, Brugge JS, Sy
Page 17 and 18: 30. Numaguchi K, Eguchi S, Yamakawa
Page 19 and 20: LEGENDS TO FIGURES Fig. 1. The asse
Page 21 and 22: 1. 2. 3. 4. 81 bp PCR 482 bp PCR 96
Page 23 and 24: Figure 3
Page 29: Figure 9

Mechanical strain regulation of the Chicken glypican-4 gene ...

Create successful ePaper yourself

Delete template?

Save as template?