Reproducible Separation of Carbohydrate, Oligosaccharide ... - NET
Reproducible Separation of Carbohydrate, Oligosaccharide ... - NET
Reproducible Separation of Carbohydrate, Oligosaccharide ... - NET
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Phenomenex ® Rezex vs. Bio-Rad ® Aminex ®<br />
mAU<br />
mAU<br />
mAU<br />
mAU<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
80<br />
60<br />
40<br />
100<br />
80<br />
60<br />
40<br />
vs. Lower Backpressure = Longer Column Lifetimes and Faster Run Time Capability<br />
Aliphatic Acids<br />
14962 & 14963<br />
Aliphatic Acids Saccharides<br />
Rezex RoA<br />
0 10 20 min<br />
Aminex ®<br />
HPX-87H<br />
Columns: Rezex RoA-organic Acid<br />
Aminex HPX-87H<br />
Dimensions: 300 x 7.8 mm<br />
Mobile Phase: 0.005 N H 2So 4<br />
Flow Rate: 0.5 mL/min<br />
Detection: UV @ 210 nm<br />
Temperature: 40 °C<br />
Sample: 1. oxalic acid 4. Succinic acid<br />
2. Citric acid 5. Formic acid<br />
3. Tartaric acid 6. Acetic acid<br />
Columns: Rezex RCM-Monosaccharide<br />
Aminex HPX-87C<br />
Dimensions: 300 x 7.8 mm<br />
Mobile Phase: Water<br />
Flow Rate: 0.6 mL/min<br />
Detection: ELSD<br />
Temperature: 80 °C<br />
Sample: 1. Sucrose 4. Fructose<br />
2. glucose 5. Sorbitol<br />
3. galactose<br />
1<br />
0 10 20 min<br />
2<br />
3<br />
4<br />
mAU<br />
mAU<br />
400<br />
300<br />
200<br />
100<br />
400<br />
300<br />
200<br />
100<br />
Columns: Rezex RPM-Monosaccharide<br />
Aminex HPX-87P<br />
Dimensions: 300 x 7.8 mm<br />
Mobile Phase: Water<br />
Flow Rate: 0.6 mL/min<br />
Detection: ELSD<br />
Temperature: 75 °C<br />
Sample: 1. Melezitose 4. Mannose<br />
2. Maltose 5. Fructose<br />
3. glucose 6. Ribitol<br />
vs. Higher Efficiency = Baseline <strong>Separation</strong> <strong>of</strong> Critical Sample Components<br />
Sugars<br />
14977 & 14978<br />
Sugars oligosaccharides<br />
0<br />
lower<br />
Backpressure<br />
Rezex RcM<br />
Aminex ®<br />
HPX-87c<br />
Rezex<br />
32<br />
Aminex<br />
55<br />
1<br />
1<br />
Bar<br />
Bar<br />
1<br />
Baseline<br />
Resolution<br />
2<br />
2<br />
3 4<br />
3<br />
4<br />
10 20<br />
30 min<br />
2 3 4<br />
0 10 20 30 min<br />
5<br />
5<br />
5<br />
5<br />
6<br />
6<br />
mAU<br />
mAU<br />
60<br />
50<br />
40<br />
30<br />
0<br />
50<br />
40<br />
30<br />
Rezex RPM<br />
lower<br />
Backpressure<br />
Aminex ®<br />
HPX-87P<br />
Dp10<br />
Dp11<br />
Dp9<br />
Dp8<br />
Dp5<br />
Dp7<br />
Dp6<br />
0 10 20 30<br />
40 min<br />
Dp8<br />
Dp9<br />
Dp10<br />
Dp11<br />
1 2<br />
Dp4<br />
Dp5<br />
Dp7 Dp6<br />
0 10 20 30<br />
40 min<br />
Columns: Rezex RSo-oligosaccharide<br />
Aminex HPX-42A<br />
Dimensions: 200 x 10.0 mm<br />
Mobile Phase: Water<br />
Flow Rate: 0.3 mL/min<br />
Detection: ELSD<br />
Temperature: 80 °C<br />
Sample: 1. Malto-oligosaccharides (Dp1-Dp14)<br />
(Dp refers to degree <strong>of</strong> polymerization)<br />
1<br />
3<br />
4<br />
10 20 25 min<br />
2<br />
3<br />
0 10 20 25 min<br />
Rezex RSo<br />
Aminex ®<br />
HPX-42A<br />
Aminex and Bio-Rad are registered trademarks <strong>of</strong> Bio-Rad Laboratories, Inc. Comparative separations may not be representative <strong>of</strong> all applications.<br />
App ID 14962<br />
App ID 14963<br />
App ID 14977<br />
App ID 14978<br />
Rezex<br />
17<br />
Aminex<br />
27<br />
Bar<br />
Bar<br />
Improved Resolution for Multiple<br />
Degrees <strong>of</strong> Polymerization<br />
4<br />
5<br />
5<br />
Dp4<br />
Dp3<br />
Dp3<br />
6<br />
6<br />
Dp2<br />
Phenomenex | Web: www.phenomenex.com<br />
Dp2<br />
Dp1<br />
Dp1<br />
App ID 14985<br />
App ID 14986<br />
App ID 14964<br />
App ID 14965<br />
5
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