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Reproducible Separation of Carbohydrate, Oligosaccharide ... - NET

Reproducible Separation of Carbohydrate, Oligosaccharide ... - NET

4 Phenomenex ® Rezex

4 Phenomenex ® Rezex vs. BiO-RAd ® AmiNEx ® mAU mAU mAU mAU 120 100 80 60 40 100 80 60 40 60 40 20 vs. Sharper Peak Shape = Easy & Accurate Quantitation vs. Saccharides orange juice 0 10 20 min 1 2 1 2 3 0 10 20 min Columns: Rezex RoA-organic Acid Aminex HPX-87H Dimensions: 300 x 7.8 mm Mobile Phase: 0.005 N H 2So 4 Flow Rate: 0.5 mL/min Detection: UV @ 210 nm Temperature: 40 °C Sample: 1. Citric acid 4. Lactic acid 2. Tartaric acid 5. Acetic acid 3. Succinic acid Superior Peak 6 1 Shape 500 easier Quantitation 3 4 4 5 5 6 mAU mAU 400 300 200 100 400 200 Rezex RcM 0 2 4 6 8 10 12 14 16 min Aminex ® HPX-87c 0 2 4 6 8 10 12 14 16 min Columns: Rezex RCM-Monosaccharide Aminex HPX-87C Dimensions: 300 x 7.8 mm Mobile Phase: Water Flow Rate: 0.6 mL/min Detection: ELSD Temperature: 80 °C Sample: 1. Sucrose 2. glucose 3. Fructose vs. vs. organic acids in wine sugar alcohols organic Acids in Wine 14968 & 14993 Sugar Alcohols 0 0 5 60 40 20 Saccharides Rezex RoA Aminex ® HPX-87c Columns: Rezex RCM-Monosaccharide Aminex HPX-87C Dimensions: 300 x 7.8 mm Mobile Phase: Water Flow Rate: 0.6 mL/min Detection: ELSD Temperature: 80 °C Sample: 1. Melezitose 4. Mannose 2. Maltose 5. Fructose 3. glucose 6. Ribitol Rezex RoA Aminex ® HPX-87H 0 0 5 1 2 1 2 3 4 3 15 4 5 5 25 min 15 25 min mAU mAU 50 40 30 50 40 30 orange Juice Sharper Peaks No Peak Fronting Columns: Rezex RPM-Monosaccharide Aminex HPX-87P Dimensions: 300 x 7.8 mm Mobile Phase: Water Flow Rate: 0.6 mL/min Detection: ELSD Temperature: 80 °C Sample: 1. glucose 4. Xylitol 2. Mannitol 5. Erythritol 3. Sorbitol 1 2 1 0 10 20 30 40 50 min Aminex ® HPX-87P Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications. Phenomenex | Web: www.phenomenex.com App ID 14975 App ID 14976 App ID 14971 App ID 14972 Rezex RPM 1 2 0 10 20 30 40 50 min 3 3 2 2 4 4 5 3 3 5 App ID 14981 App ID 14982 App ID 14968 App ID 14993

Phenomenex ® Rezex vs. Bio-Rad ® Aminex ® mAU mAU mAU mAU 120 100 80 60 40 20 0 120 100 80 60 40 20 0 80 60 40 100 80 60 40 vs. Lower Backpressure = Longer Column Lifetimes and Faster Run Time Capability Aliphatic Acids 14962 & 14963 Aliphatic Acids Saccharides Rezex RoA 0 10 20 min Aminex ® HPX-87H Columns: Rezex RoA-organic Acid Aminex HPX-87H Dimensions: 300 x 7.8 mm Mobile Phase: 0.005 N H 2So 4 Flow Rate: 0.5 mL/min Detection: UV @ 210 nm Temperature: 40 °C Sample: 1. oxalic acid 4. Succinic acid 2. Citric acid 5. Formic acid 3. Tartaric acid 6. Acetic acid Columns: Rezex RCM-Monosaccharide Aminex HPX-87C Dimensions: 300 x 7.8 mm Mobile Phase: Water Flow Rate: 0.6 mL/min Detection: ELSD Temperature: 80 °C Sample: 1. Sucrose 4. Fructose 2. glucose 5. Sorbitol 3. galactose 1 0 10 20 min 2 3 4 mAU mAU 400 300 200 100 400 300 200 100 Columns: Rezex RPM-Monosaccharide Aminex HPX-87P Dimensions: 300 x 7.8 mm Mobile Phase: Water Flow Rate: 0.6 mL/min Detection: ELSD Temperature: 75 °C Sample: 1. Melezitose 4. Mannose 2. Maltose 5. Fructose 3. glucose 6. Ribitol vs. Higher Efficiency = Baseline Separation of Critical Sample Components Sugars 14977 & 14978 Sugars oligosaccharides 0 lower Backpressure Rezex RcM Aminex ® HPX-87c Rezex 32 Aminex 55 1 1 Bar Bar 1 Baseline Resolution 2 2 3 4 3 4 10 20 30 min 2 3 4 0 10 20 30 min 5 5 5 5 6 6 mAU mAU 60 50 40 30 0 50 40 30 Rezex RPM lower Backpressure Aminex ® HPX-87P Dp10 Dp11 Dp9 Dp8 Dp5 Dp7 Dp6 0 10 20 30 40 min Dp8 Dp9 Dp10 Dp11 1 2 Dp4 Dp5 Dp7 Dp6 0 10 20 30 40 min Columns: Rezex RSo-oligosaccharide Aminex HPX-42A Dimensions: 200 x 10.0 mm Mobile Phase: Water Flow Rate: 0.3 mL/min Detection: ELSD Temperature: 80 °C Sample: 1. Malto-oligosaccharides (Dp1-Dp14) (Dp refers to degree of polymerization) 1 3 4 10 20 25 min 2 3 0 10 20 25 min Rezex RSo Aminex ® HPX-42A Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications. App ID 14962 App ID 14963 App ID 14977 App ID 14978 Rezex 17 Aminex 27 Bar Bar Improved Resolution for Multiple Degrees of Polymerization 4 5 5 Dp4 Dp3 Dp3 6 6 Dp2 Phenomenex | Web: www.phenomenex.com Dp2 Dp1 Dp1 App ID 14985 App ID 14986 App ID 14964 App ID 14965 5

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