EURON and THEME joint PhD meeting

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46 EURON and THEME joint meeting 2011 mechanisms are not understood. Analysis of GABA B R1 knockout mice will help to better understand how Ca 2+ signaling in astrocytes influences information processing in the brain.
47 EURON and THEME joint meeting 2011 Neurogenomics in perinatal asphyxia and fetal preconditioning KEM Cox, E Strackx, E Vlassaks, M Sparnaaij, L Zimmerman, JS Vles, AW Gavilanes. Dept of Psychiatry and Neuropsychology, Maastricht University, The Netherlands. Background Part of perinatal hypoxic-ischemic brain damage can be attenuated when it is preceded by fetal preconditioning. The mechanism behind this endogenous neuroprotective phenomenon has not been deciphered yet. It is suggested that genomic reprogramming could be the answer to this question. We performed 50 micro array experiments to evaluate changes in cerebral gene expression after fetal preconditioning and perinatal asphyxia at multiple time points. In this abstract we describe some preliminary results. Objective We aim to analyze the whole genome gene expression at multiple time points in pre- and neonatal pups after fetal preconditioning (FA) and perinatal asphyxia (PA). This can give us insight into the mechanisms of endogenous neuroprotection after FA and the deleterious effects of PA. Unraveling these mechanisms is an important step towards possible new clinical strategies for asphyctic neonates. Design/Methods FA was induced on E17 by clamping the uterine circulation of the maternal rat for 30 minutes. On P0 pups PA was induced by placing the uterine horns, including the fetuses, in a water bath for 19 minutes. Control (CCD) and FA pups were delivered by Caesarean section. These procedures generated four experimental groups: CCD, FA, PA, and FA+PA. Five male pups per group were sacrificed at the following time points: 96h after FA, 6h and 96h after birth. RNA was isolated from the left hemispheres and 50 micro array experiments were performed on the Affymetrix platform. Up- or down-regulation of gene-products was studied via Gene-Ontology terms with a computational method named Gene Set Enrichment Analysis (GSEA). Results With GSEA we found a considerable number of GO-terms that were significantly up- or down-regulated compared to CCD animals (see figure 1). Interestingly in the protected phenotype (FA+PA) no GO-terms were significantly different from the control phenotype 6 hours after birth. Four days after perinatal asphyxia we
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Page 29 and 30: THEME Lecture Eckhard Mandelkow POS
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List of Participants 103 EURON and
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Nuersailike Abuduwali University of
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Marianne Eisenhardt University of B
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Sabine Klein University of Bonn PhD
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Viktoriya Peeva University of Bonn
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Sylvie van der Kruijs Maastricht Un
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EURON and THEME joint PhD meeting

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