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57 Mechanism of membrane fusion and the release of hormones and transmitters James Rothman 1 . Yale University, New Haven, CT 1 Abstract: Intracellular membrane fusion is catalyzed by the zippering of SNARE proteins into helical bundles (termed trans-SNARE complexes, or SNAREpins) between membranes, forcing their bilayers together. In synaptic transmission, fusion does not occur until calcium enters the presynaptic cytoplasm. Recent studies shed light both on the biophysics of fusion and on how it is regulated for precise release of neurotransmitters and hormones.
58 Ctage5 is involved in the endoplasmic reticulum to Golgi trafficking in mammalian cells Kota Saito 1 , Koh Yamashiro 1 , Yuki Ichikawa 1 , Patrik Erlmann 2 , Vivek Malhotra 2 , Toshiaki Katada 1 . Department of Physiological Chemistry, School of Pharmaceutical Sciences, University of Tokyo, Japan 1 , Center for Genomic Regulation, Barcelona, Spain 2 Abstract: Secretory proteins are synthesized in the endoplasmic reticulum (ER) and transported to the ER-Golgi intermediate compartment (ERGIC) via COPII-coated vesicles. Although the minimal components required for the coated-vesicle formation are relatively well characterized, less is known about how the cargoes are selected into nascent vesicles. Recently we identified TANGO1, an integral membrane protein localized on the ER exit sites. Although TANGO1 is not required for general protein secretion, it is specifically required for collagen exit from the ER. Thus, TANGO1 is a new type of cargo receptor conserved throughout metazoans. cTAGE5, cutaneous T-cell lymphoma-associated antigen, has been shown to be overexpressed in several cancer tissues including T-cell lymphoma, meningioma and melanoma, and regarded as a tumor antigen candidate. However, the physiological characterization of the protein has not been reported so far. Here we show that endogenous cTAGE5 is specifically localized to the ER exit sites. Knockdown of cTAGE5 by RNA interference disrupts ERGIC and Golgi structure, although it does not affect the Vesicular Stomatitis Virus Glycoprotein-ts045 traffic to the plasma membrane. Northern blot analysis revealed that cTAGE5 is ubiquitously expressed in normal human tissues. cTAGE5 contains a signal anchor motif, single trans-membrane domain, two coiled-coil regions and Proline Rich domain from the N-terminus; structurally resemble to the C-terminal half of TANGO1. Interestingly, knockdown of cTAGE5 results in collagen VII accumulation in the ER. Moreover, cTAGE5 can co-immunoprecipitate with TANGO1. Functional relationship between cTAGE5 and TANGO1 will be discussed.
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October 28 - October 31, 2010 Bioch
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Symposium Agenda Thursday, October
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12:10 p.m. - 4:00 p.m. Lunch and Fr
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4:50 p.m. - 5:00 p.m. Discussion 5:
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Poster Presentations Last Names A -
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The Role of Ceroid Lipofuscinosis N
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A novel Golgi-localized protein inv
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2 The dynamics of SNARE complexes i
Page 17 and 18: 4 Significant divergence in mammali
Page 19 and 20: 6 The Tumoricidal Action of the Ade
Page 21 and 22: 8 LMTK2 Regulates CFTR Recycling in
Page 23 and 24: 10 Sorting in the Golgi Apparatus C
Page 25 and 26: 12 Negative regulation of the endoc
Page 27 and 28: 14 Mechanistic details of sorting n
Page 29 and 30: 16 An ESCRTs View of Receptor Endoc
Page 31 and 32: 18 GOLPH3 Bridges PtdIns(4)P and My
Page 33 and 34: 20 Novel fluorescent probes to stud
Page 35 and 36: 22 Regulation of Ligand-Independent
Page 37 and 38: 24 A Fifth Adaptor Protein Complex
Page 39 and 40: 26 CATCHR-Family Tethering Complexe
Page 41 and 42: 28 Rab7 localization and activity a
Page 43 and 44: 30 The Drosophila Golgi transmembra
Page 45 and 46: 33 Cbl Amino Acids at a Putative Di
Page 47 and 48: 35 The role of COG subcomplexes in
Page 49 and 50: 37 Mechanism of secretory cargo sor
Page 51 and 52: 39 Understanding the mechanism of G
Page 53 and 54: 41 Mechanisms for selective activat
Page 55 and 56: 44 A novel coat complex traffics me
Page 57 and 58: 46 Membrane trafficking in cytokine
Page 59 and 60: 48 Phosphatidylinositol 4-phosphate
Page 61 and 62: 50 Control of Golgi function by Rab
Page 63 and 64: 52 Adenosine mediated modulation of
Page 65 and 66: 54 Syp1 regulation of actin polymer
Page 67: 56 Rap1A: A Novel Interactor Involv
Page 71 and 72: 60 A vesicle carrier that mediates
Page 73 and 74: 62 Structural Insights into Dynamin
Page 75 and 76: 64 Identification and interaction m
Page 77 and 78: 66 hRME-6, a rab5GEF that integrate
Page 79 and 80: 68 Assembly of caveolin-1 and cavin
Page 81 and 82: 70 Opposing roles of Annexin A1 and
Page 83 and 84: 72 The role of SNX-BAR proteins in
Page 85 and 86: 74 TRAPP is required for ER exit of
Page 87 and 88: 76 A structural analysis of the ER
Page 89 and 90: 78 Cell cycle-regulated Golgi stack
Page 91 and 92: 80 Rab 14 regulates tight junction
Page 93 and 94: 82 Dissecting the roles of O-glycos
Page 95 and 96: Author Index - A- Abay, S., 33 Acha
Page 97 and 98: Marsico, G., 83 Mattera, R., 11 Max
Page 99: 2011 ASBMB Special Symposia Series
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