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54 58<br />

YIELD CONSTRAINTS IN WHEAT DUE TO SOILBORNE PATHOGENS. PERSPECTIVES OF DISEASE THREAT IN LARGE-SCALE PINUS RADIATA<br />

E.A. Milus and C.S. Rothrock, Dept. of Plant Pathology, MONOCULTURE -THE NEW ZEALAND EXPERIENCE. C.K.S.Chou, Forest<br />

University of Arkansas, Fayetteville, AR 72701. Research Institute, Rotorua, New Zealand.<br />

About 95% of New Zealand wood production today comes from exotic<br />

The importance of soilborne pathogens as constraints to plantations (mainly Pinus radiata) .For over 6 decades this forestry<br />

wheat yield and quality in Arkansas was determined in a monoculture,now 1.1 million hahas withstood the threat of dissplit,<br />

strip plot experiment. Main plots were raised or ease. In the late 1940's 25-35% of 20-25-year-old trees (unthinned<br />

conventional seed beds. Subplots were fumigated (Bromogas stands) were killed in a Sirex-Amylostereum epidemic.However the<br />

67), metalaxyl-treated seed, and a nontreated check. Sub- economic loss was insignificant.Aerial spraying to control<br />

subplots were planted with wheat (cv. Keiser, Florida 302, Dothistroma needle blight since 1966 has cost around $14.5<br />

or Caldwell) or oats (cv. Bob). In January plants were dug million. Losses caused by other diseases, i.e.,Sphaeropsis sapinea<br />

from the ends of each plot, growth parameters were measured, Armillaria root-rot,and Cyclaneusma needle-cast, have been<br />

and the entire crown and root system plated onto water agar. locally severe but tolerable overall. The current practice of waste<br />

Fungal colonies were transferred to PDA, identified to genus, thinning 75- 8 5% of initial stockings before age 10-12 allows a high<br />

and representative isolates were tested for pathogenicity. level of acceptable loss for diseases which mainly attack young<br />

Bedded plots had twice the number of plants infected with stands, while clear-felling at age 25-30 obviates diseases that<br />

Helminthosporium sativum. Plants from fumigated plots had affect older stands.However, New Zealand still has only a few pine<br />

more tillers/plant, greater top weight, and fewer plants pathogens and with the trend towards clonal forestry, genetic uniinfected<br />

with Pythium, Helminthosporium sativum, and formity, and reduction of initial/final crop stocking ratios,the<br />

Rhizoctonia. Pythium was the most commo .genus isolated. history of disease in this country is no way near its end.<br />

55<br />

HYPOVIRULENT STRAINS OF CRYPHONECTRIA PARASITICA IN<br />

RECOVERY<br />

NEW JERSEY.<br />

OF WHITE AND GREEN ISOLATES OF ASPERGILLUS FLAVUS P.J. Bedker. Department of Horticulture<br />

FROM<br />

and<br />

INDIVIDUAL<br />

Forestry,<br />

KERNELS<br />

Rutgers<br />

FROM DOUBLE-INOCULATED MAIZE EARS. University, New Brunswick, NJ 08903.<br />

Natale Zummo, USDA-ARS, Dept. of Plant Pathology and Weed<br />

Science, Mississippi State, MS 39762. Approximately 30 isolates of C. parasitica were collected from<br />

cankers<br />

Maize on ears were<br />

<strong>American</strong> chestnut inoculated at 4 locations. in the field The with virulence a white of<br />

each was determined by inoculating<br />

isolate of<br />

"Granny<br />

Aspergillus<br />

Smith"<br />

flavus<br />

apple<br />

at<br />

fruits.<br />

2 days after midsilk in Mean radial growth (MRG) was determined<br />

the shank or<br />

by<br />

through<br />

measuring<br />

the<br />

the<br />

husk<br />

length<br />

in the base of the cob and and width of lesions at 17 days after inoculation for 8<br />

re-inoculated 4 days later with a green isolate of the replications / isolate. Each apple was also inoculated with<br />

fungus through the husk at the top<br />

silk<br />

of the<br />

channel.<br />

ear or in the<br />

Reciprocal<br />

strain<br />

inoculations<br />

EP155 from Connecticut.<br />

were made.<br />

The MRG<br />

Ears<br />

for isolates collected<br />

from sites in Howell and Millstone Twps.<br />

were<br />

(Monmouth<br />

harvested at<br />

Co.),<br />

60 days after midsilk, dried, and Plumsted Twp. (Ocean Co.), and Sandyston Twp. (Sussex<br />

shelled. When<br />

Co.)<br />

kernels<br />

were<br />

from these double-inoculated ears 18.2, 19.1, 17.3, and 18.4 mm, respectively. The<br />

were<br />

MRG for<br />

assayed,<br />

37.5<br />

the<br />

%<br />

white isolate was recovered singly from (45/120) of the isolates was significantly<br />

8% of kernels,<br />

(P

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