Microbiological Process Optimization of Biogas Synthesis
Microbiological Process Optimization of Biogas Synthesis
Microbiological Process Optimization of Biogas Synthesis
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Improved molecular strategies to assess<br />
community activity and dynamics<br />
� RNA is a more sensitive marker <strong>of</strong> activity changes<br />
compared to DNA<br />
� Functional gene approach provides information on<br />
the structure <strong>of</strong> certain functional guilds<br />
�Methanogens – mcrA (Methyl coenzym M reductase)<br />
�Acetogens – fhs (Formyltetrahydr<strong>of</strong>olate synthetase)<br />
�Sulfate reduction – dsrAB (Dissimilatory sulfite reductase)<br />
� Cellulase and hydrogenase genes<br />
� …<br />
�Strategy: Study expression <strong>of</strong> functional genes<br />
Combination with flow cytometry, isotope tools,<br />
metabolomics, modeling
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