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Postharvest treatments to reduce chilling injury symptoms in stored ...

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temperatures with one or more exposure <strong>to</strong> non-<strong>chill<strong>in</strong>g</strong> (higher) temperatures.<br />

Rewarm<strong>in</strong>g allows metabolism of the <strong>to</strong>xic products which accumulates <strong>in</strong> the tissues at<br />

low temperature (Pentzer and He<strong>in</strong>z, 1954) and also res<strong>to</strong>res certa<strong>in</strong> depleted essential<br />

metabolites. Rewarm<strong>in</strong>g also allows the revival of oxidative phosphorylation <strong>in</strong> tissues as<br />

it gets suppressed at low temperatures.<br />

Temperature condition<strong>in</strong>g <strong>in</strong>volves s<strong>to</strong>rage of products above the <strong>chill<strong>in</strong>g</strong><br />

threshold temperature before they are s<strong>to</strong>red at a low temperature (Wade, 1979). This<br />

may be a s<strong>in</strong>gle step operation or may be achieved <strong>in</strong> multi-steps by gradually decreas<strong>in</strong>g<br />

the temperature. However, a s<strong>in</strong>gle step condition<strong>in</strong>g is less effective than a multi-step<br />

condition<strong>in</strong>g.<br />

Jacobi et al., (1995, 1996) reported that condition<strong>in</strong>g of mango fruit cv.<br />

Kens<strong>in</strong>g<strong>to</strong>n at 40°C <strong>in</strong>creased heat <strong>to</strong>lerance and <strong>reduce</strong>d the effect of heat <strong><strong>in</strong>jury</strong>.<br />

Accord<strong>in</strong>g <strong>to</strong> these reports, those fruits which received HW condition<strong>in</strong>g treatment at 40<br />

ºC for 8-12 h had m<strong>in</strong>imum heat <strong><strong>in</strong>jury</strong> and showed <strong>in</strong>ternal and external cavities and<br />

starchy layer beneath the sk<strong>in</strong>. Heat-treatment effectively <strong>in</strong>creases fruit heat <strong>to</strong>lerance<br />

and <strong>reduce</strong>s CI <strong>in</strong> s<strong>to</strong>red avocado (Woolf et al., 1995). There are different techniques<br />

which are used <strong>to</strong> m<strong>in</strong>imize the <strong>in</strong>cidence of CI.<br />

2.5.1 Use of heat-treatment<br />

Different heat-<strong>treatments</strong> have been used <strong>to</strong> alleviate the <strong>in</strong>cidence of CI<br />

symp<strong>to</strong>ms <strong>in</strong> mango fruits. VHT-treated mango fruits showed <strong>in</strong>creased level of<br />

putresc<strong>in</strong>e and its accumulation helped the reduction of CI (Esguera and Lizada, 1990).<br />

As stated by González et al. (2000) peppers treated with HW at 53 °C for 4 m<strong>in</strong>, had a<br />

<strong>reduce</strong>d <strong>in</strong>cidence of CI after 14 and 28 days s<strong>to</strong>rage at 8 °C. It has been hypothesized<br />

that HW-treated fruits show <strong>in</strong>creased level of polyam<strong>in</strong>e which <strong>reduce</strong>s CI symp<strong>to</strong>ms <strong>in</strong><br />

fruits (González, 1997).<br />

Ethylene-treated fruits, irrespective of the method of application, cause severe<br />

mesocarp discoloration <strong>in</strong> avocado fruits. Loss of green color has been observed <strong>in</strong> plant<br />

tissues while they are treated with Ethylene. The treatment also decreases fruit firmness,<br />

and CI dur<strong>in</strong>g prolonged s<strong>to</strong>rage (Watada, 1986). The mechanism by which ethylene<br />

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