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Sylvain_Détermination de la concentration en tryptophane

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Comparaison <strong>en</strong>tre les mesures effectuées<br />

par HPLC et celles par spectroscopie <strong>de</strong><br />

fluoresc<strong>en</strong>ce, et <strong>de</strong> leurs erreurs<br />

• HPLC<br />

– Moins s<strong>en</strong>sible: DL <strong>de</strong> l’ordre <strong>de</strong> 1,5.10 -8 mol/L<br />

– moins précis <strong>de</strong> ±3.56% jusqu’à ±30.52%<br />

• Spectroscopie <strong>de</strong> fluoresc<strong>en</strong>ce synchrone<br />

– S<strong>en</strong>sible à partir <strong>de</strong> 0,82.10 -8 mol/L<br />

– Marge d’erreur <strong>de</strong> ±0,37% à ±2,45%

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