Arbeitsanleitung - Milenia Biotec GmbH

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- 4 - M ethod and Test Principle In combination with the Milenia POCScan Reader the Milenia QuickLine Total IgE is a lateral flow immunoassay which allows the quantitative determination of total immunoglobulin E (IgE) from whole blood. Immediately after pipetting the whole blood sample to sample port of the test cassette, one drop of assay buffer is added. IgE antibodies of the patient sample then specifically bind to the ligand-labeled polyclonal anti-IgE antibodies present in the assay buffer. These immune complexes migrate into the test strip and there associate with the gold conjugate, which are coated with monoclonal anti-IgE antibodies. The gold particles overflow the test line (T) where an anti-ligand is immobilized. Only the immune complexes consisting of ligand labeled anti-IgE antibodies, patient’s IgE and the gold conjugate will bind to the antiligand. Over time a reddish test band is generated. Gold conjugat-specific antibodies, which are printed as a line on the membrane (control line, C), catch unbound gold conjugat; so a clearly visible line is generated during the test time. Interpretation of results is only possible with the Milenia POCScan Reader, which allows an objective interpretation and documentation of the result. The total IgE concentration directly correlates with the color intensity of the test band (T). The Milenia POCScan Reader measures the color reflection of the test band and calculates the result according a programmed standard curve. Milenia Biotec, Versailler Str. 1, 35394 Gießen, Germany Milenia QuickLine Total IgE (MQTE)
- 5 - M ilenia PO CScan Reader O verview Display Test Cassette Drawer BACK Button ENTER and ON/OFF Button UP Button Battery Compartment DOWN Button FORWARD Button I/O Port for Printer and Barcode Reader USB Port Power Jack Battery position and replacement: _ + _ + _ + - Please use Nickel-Metal-Hybride (NiMH) batteries only, which offer longer durability. - ATTENTION: Recharging of batteries is working only, if POCScan reader is switched ON! Milenia Biotec, Versailler Str. 1, 35394 Gießen, Germany Milenia QuickLine Total IgE (MQTE)
Page 1 and 2: Total IgE Lateral Flow Immunoassay
Page 3: - 3 - M aterials Supplied, Storage
Page 7 and 8: - 7 - Step 5. Scan Mode Lot and Pro
Page 9 and 10: - 9 - C) TEST Step 1. Pipet the pat
Page 11 and 12: - 11 - Interpretation of Results Th
Page 13 and 14: - 13 - Total IgE Lateral Flow Immun
Page 15 and 16: - 15 - Kitbestandteile, Lagerung un
Page 17 and 18: - 17 - M ilenia PO CScan Reader Ü
Page 19 and 20: - 19 - Schritt 5. Scan Mode Lot and
Page 21 and 22: - 21 - C) TEST Schritt 1. Das Patie
Page 23 and 24: - 23 - Ausw ertung der Ergebnisse D
Page 25 and 26: - 25 - Total IgE Lateral Flow Immun
Page 27 and 28: - 27 - M atériel fourni, Conservat
Page 29 and 30: - 29 - M ilenia PO CScan Reader -
Page 31 and 32: - 31 - Étape 5. Scan Mode Lot and
Page 33 and 34: - 33 - C) TEST Étape 1. Pipette le
Page 35: - 35 - Interprétation des résulta

Arbeitsanleitung - Milenia Biotec GmbH

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