A.2Désignation produit Réf. Conditionnement Tarif Cat HT Remise Tarif EPST HT CommentairePage CatBioScienceELECTROPHORESEGelsAcrylami<strong>de</strong>, solution 40%........................................................................................ UP873376................. 500 ml................. 40,50............... 10%....... 36,00................. Purité Acrylami<strong>de</strong> >99,9%.......................................................................... B194, D77Acrylami<strong>de</strong>:bis-acrylami<strong>de</strong> 19:1, solution 40%, Ultra Pure Gra<strong>de</strong>........................... UP86489B................. 500mL................. 40,50............... 10%....... 36,00................. Purité Acrylami<strong>de</strong> >99,9%.......................................................................... D.77Acrylami<strong>de</strong>:bis-acrylami<strong>de</strong> 29:1, solution 40%, Ultra Pure Gra<strong>de</strong>........................... UP864927................. 500mL................. 40,50............... 10%....... 36,00................. Purité Acrylami<strong>de</strong> >99,9%.......................................................................... B194, D77Acrylami<strong>de</strong>:bis-acrylami<strong>de</strong> 37,5:1, solution 40%, Ultra Pure Gra<strong>de</strong>........................ UP864937................. 500mL................. 40,50............... 10%....... 36,00................. Purité Acrylami<strong>de</strong> >99,9%.......................................................................... B194Agarose, regular uses............................................................................................. 31272L...................... 500g.................... 266,50............. 10%....... 239,50............... Standard, lecture claire, séparation rapi<strong>de</strong>................................................ NouveauAgarose I................................................................................................................. 312722...................... 500g.................... 403,50............. 10%....... 363,00............... Standard..................................................................................................... D81Agarose II................................................................................................................ 284491...................... 100g.................... 277,00............. 10%....... 249,00............... Low melting................................................................................................ D81Agarose III............................................................................................................... 900781...................... 100g.................... 147,50............. 10%....... 132,50............... Electrophorèse champ pulsé...................................................................... D81Agarose 3:1............................................................................................................. 327280...................... 100g.................... 186,00............. 10%....... 167,00............... fragments 98%............................................................................................... B195TEMED.................................................................................................................... UP15413D................. 25mL................... 13,50............... 10%....... 12,00................. Ultra Pure Gra<strong>de</strong>........................................................................................ B195TamponsAci<strong>de</strong> borique........................................................................................................... UP070440................. 1 kg..................... 30,50............... 10%....... 27,00................. ACS gra<strong>de</strong>, DNase, RNase, Protease free................................................ D91Aci<strong>de</strong> citrique........................................................................................................... UP168781................. 1 kg..................... 23,50............... 10%....... 21,00................. ACS gra<strong>de</strong>, DNase, RNase, Protease free................................................ D91DTT.......................................................................................................................... UP284250................. 1 g....................... 11,00............... 10%....... 9,50................... Biotechnology Gra<strong>de</strong>.................................................................................. D91EDTA....................................................................................................................... UP036290................. 1 kg..................... 71,00............... 10%....... 63,50................. Biotechnology Gra<strong>de</strong>.................................................................................. D92Glycine..................................................................................................................... UP018225................. 1kg...................... 30,50............... 10%....... 27,00................. Biotechnology Gra<strong>de</strong>.................................................................................. D92HEPES free acid...................................................................................................... UP061940................. 250 g................... 89,00............... 10%....... 80,00................. Ultra Pure Gra<strong>de</strong>, DNase, RNase, Protease free...................................... D92MOPS...................................................................................................................... UP062000................. 100g.................... 36,00............... 10%....... 32,00................. Ultra Pure Gra<strong>de</strong>........................................................................................ D92PBS poudre, 1 pack = 1 L <strong>de</strong> 10X........................................................................... UP68723A................. 1 pack.................. 13,50............... 10%....... 12,00................. ................................................................................................................... D93PBS solution 10X..................................................................................................... N14010...................... 4L........................ 99,00............... 10%....... 89,00................. ................................................................................................................... D93SDS poudre............................................................................................................. UP649100................. 500g.................... 98,00............... 10%....... 88,00................. ................................................................................................................... D93SDS solution 20%.................................................................................................... UP896826................. 500mL................. 56,00............... 10%....... 50,00................. ................................................................................................................... D93TAE poudre, qsp 40L............................................................................................... 892580...................... 1 pk..................... 67,50............... 10%....... 60,50................. ................................................................................................................... D90TAE solution 25X..................................................................................................... UP892574................. 1,6L..................... 51,00............... 10%....... 45,50................. ................................................................................................................... D90TBE poudre, qsp 40L............................................................................................... 892533...................... 1 pk..................... 53,50............... 10%....... 48,00................. ................................................................................................................... D90TBE solution 10X..................................................................................................... UP86510H................. 4L........................ 49,00............... 10%....... 44,00................. ................................................................................................................... D90TBS poudre, pack qsp 20L...................................................................................... UP74004A................. 1 pk..................... 15,00............... 10%....... 13,50................. ................................................................................................................... D94TBS solution 20X..................................................................................................... N14580...................... 4L........................ 96,00............... 10%....... 86,00................. ................................................................................................................... D94TG poudre, pack qsp 40L........................................................................................ 914940...................... 1 pk..................... 37,00............... 10%....... 33,00................. ................................................................................................................... B201TG solution 10X....................................................................................................... 914930...................... 4L........................ 60,50............... 10%....... 54,00................. ................................................................................................................... B201TG-SDS poudre....................................................................................................... 865980...................... QSP 40L.............. 38,50............... 10%....... 34,50................. ................................................................................................................... B201TG-SDS solution 10X.............................................................................................. UP914957................. 4L........................ 58,50............... 10%....... 52,50................. ................................................................................................................... B201TRIS base................................................................................................................ UP031657................. 1 kg..................... 55,50............... 10%....... 49,50................. RNase, DNase, protease free.................................................................... D94TRIS HCl................................................................................................................. UP09154E................. 1kg...................... 92,50............... 10%....... 83,00................. RNase, DNase, protease free.................................................................... D94Urée......................................................................................................................... UP031904................. 1kg...................... 52,00............... 10%....... 46,50................. Ultra Pure Gra<strong>de</strong>........................................................................................ D94Eau, traitée au DEPC.............................................................................................. 457420...................... 500mL................. 27,00............... 10%....... 24,00................. Nuclease, RNase free, stérile.................................................................... D94Marqueurs <strong>de</strong> tailleADN 1 kb lad<strong>de</strong>r UPS54802................. 100µg.................. 65,00............... 10%....... 58,50................. 200 tests..................................................................................................... D84ADN 100pb lad<strong>de</strong>r UPS54811................. 50µg.................... 50,50............... 10%....... 45,00................. 100 tests..................................................................................................... D84Protéines Low Range 3,5-31 kD (6 ban<strong>de</strong>s) 587231...................... 200µL.................. 98,00............... 10%....... 88,00................. 40 tests....................................................................................................... B199Protéines Precise 15-150 kD (7 ban<strong>de</strong>s) N14020...................... 200µL.................. 69,00............... 10%....... 62,00................. 40 tests....................................................................................................... B199Protéines Large Range 14-212 kD (8 ban<strong>de</strong>s) BB7080..................... 500µL.................. 89,00............... 10%....... 80,00................. 100 à 150 tests........................................................................................... B199Proteines multi-colorées 16-213KDa (7 ban<strong>de</strong>s) L77151...................... 500 µl.................. 154,50............. 10%....... 139,00............... 50 à 100 tests.............................................................................................Révélation Aci<strong>de</strong>s nucléiquesBromure d’éthidium................................................................................................. UP89244B................. 10mL................... 22,50............... 10%....... 20,00................. 10mg/mL.................................................................................................... D88Bromure d’éthidium (compte goutte)....................................................................... 327900...................... 5mL..................... 18,00............... 10%....... 16,00................. 1 goutte / 50 mL <strong>de</strong> gel.............................................................................. D88GelRed 10000X (dans l’eau)................................................................................... BY1740..................... 500µL.................. 134,00............. 10%....... 120,50............... lecture table UV (moins toxique que BET)................................................. D87GelGreen 10000X (dans l’eau)................................................................................ CJ2730...................... 500µL.................. 120,50............. 10%....... 108,00............... Fluorescence verte (+ stable que Sybr green TM)..................................... NouveauDestaining bags....................................................................................................... 988422...................... 25 U..................... 71,50............... 10%....... 64,00................. retient jusqu’à 5mg <strong>de</strong> BET par sac........................................................... D88Tel 33 (0)4 70 03 88 55 Hot line 33 (0)4 70 03 73 06 Fax 33 (0)4 70 03 82 60
Désignation produit Réf. Conditionnement Tarif Cat HT Remise Tarif EPST HT CommentairePage CatBioScienceRévélation ProtéinesCooBlue FX, coomassie protein gel stain................................................................ UP47255A................. 500mL................. 53,50............... 20%....... 42,50................. coomassie colloïdal - sensibilité 10 ng....................................................... B204Nitrate d’argent........................................................................................................ 084961...................... 25g...................... 50,50............... 10%....... 45,00................. ................................................................................................................... B208Coomassie R Brilliant Blue R-250........................................................................... 11525A...................... 10g...................... 22,50............... 10%....... 20,00................. Le plus utilisé - sensibilité 0,1 µg............................................................... B206Coomassie R Brilliant Blue G-250........................................................................... 115243...................... 10g...................... 21,50............... 10%....... 19,00................. Le plus rapi<strong>de</strong> - sensibilité 0,5 µg.............................................................. B206BIOLOGIE MOLECULAIREBiochimiquesChloroforme............................................................................................................. UP046904................. 950mL................. 35,50............... 10%....... 31,50................. Pureté 99,9%............................................................................................. D13Chloroforme:Alcool Isoamylique 24:1...................................................................... UP899255................. 950mL................. 39,50............... 10%....... 35,50................. Pureté 99,9%............................................................................................. D12Chlorure <strong>de</strong> Césium................................................................................................. 583022...................... 100g.................... 62,00............... 10%....... 55,50................. Isolation d’aci<strong>de</strong>s nucléiques dans un gradient <strong>de</strong> <strong>de</strong>nsité........................ D14Chlorure <strong>de</strong> Lithium 8M, sterile solution.................................................................. GS3320..................... 100mL................. 30,00............... 10%....... 27,00................. Elimine les inhibiteurs <strong>de</strong> la synthèse d’ADNc........................................... D15Deoxyribonuclease I................................................................................................ T06510...................... 2500U.................. 118,00............. 20%....... 94,00................. RNase, Protease free................................................................................. D17Guanidine HCL........................................................................................................ 01838L...................... 250g.................... 64,50............... 10%....... 58,00................. Inactive les RNases et protéases - Proteomics Gra<strong>de</strong>.............................. D14Guanidine thiocyanate............................................................................................. 12829F...................... 250g.................... 81,00............... 10%....... 72,50................. Inactive les Rnases. RNase, DNase, protease free................................... D15Lysozyme................................................................................................................ 13617B...................... 10g...................... 71,50............... 10%....... 64,00................. Activité >20 kU/mg. Lyse bactérienne........................................................ D18Phénol:Chloroforme:Alcool Isoamylique 25:24:1 pH 6,7/8,0.................................. UP873358................. 500 ml................. 71,00............... 10%....... 63,50................. Elimine les protéines <strong>de</strong>s préparations d’ADN........................................... D13Phénol:Chloroforme:Alcool Isoamylique 25:24:1 pH 4,5........................................ UP873357................. 100mL................. 30,00............... 10%....... 27,00................. Purification d’ARN en une étape................................................................ D13Phénol:Chloroforme:Alcool Isoamylique 25:24:1 pH 5,2........................................ 574421...................... 400mL................. 99,50............... 10%....... 89,50................. Recupération maximum d’ARN poly(A+) -> ARNm.................................... D17Phénol liqui<strong>de</strong> USP gra<strong>de</strong>....................................................................................... N13410...................... 500mL................. 43,50............... 10%....... 39,00................. pureté > 99,0%........................................................................................... D12Phénol saturé pH 4,5............................................................................................... UP893306................. 400mL................. 67,00............... 10%....... 60,00................. pureté 99,9%.............................................................................................. D13Phénol saturé pH 6,6-7,9......................................................................................... UP87336B................. 400mL................. 77,50............... 10%....... 69,50................. pureté 99,9%.............................................................................................. D12Protéinase K, solution 20 mg/ml.............................................................................. 718960...................... 5 ml..................... 93,50............... 10%....... 84,00................. Activité ≥ 30 kU/mg. Protéase non spécifique........................................... D18RNase A, solution 10 mg/ml, pH 8.0........................................................................ 734121...................... 5mL..................... 50,50............... 10%....... 45,00................. Hydrolyse les ARN contaminants............................................................... D18RNase inhibitor........................................................................................................ 973990...................... 2000U.................. 84,50............... 10%....... 76,00................. 1U inhibe 50% <strong>de</strong> l’activité <strong>de</strong> 5 ng <strong>de</strong> RNase A........................................ D18Solution <strong>de</strong> lyse 0,2 N NaOH et 1% SDS................................................................ N14440...................... 500mL................. 72,00............... 10%....... 64,50................. Lyse alcaline <strong>de</strong>s bactéries........................................................................ D15PCRUptitherm (MgCl2 à part)......................................................................................... UPS53921 *.............. 1000U.................. 92,50............... 20%....... 74,00................. pour PCR <strong>de</strong> routine; 5U/µL...................................................................... D21Uptitherm (MgCl2 inclus dans tampon)................................................................... UPS53881 *.............. 1000U ................. 92,50............... 20%....... 74,00................. pour PCR <strong>de</strong> routine; 5U/µL...................................................................... D21Uptitherm (MgCl2 inclus dans tampon) + dNTP...................................................... UPS53763 *.............. 1000U ................. 185,50............. 20%....... 148,00............... pour PCR <strong>de</strong> routine; 5U/µL...................................................................... D21dNTP set.................................................................................................................. UP968640 *............... 4x25µmol............ 92,50............... 20%....... 74,00................. dATP, dGTP, dCTP, dTTP, 250 µl (100 mM) chacun.................................. D25AMV reverse transcriptase...................................................................................... N14050 *................... 200 U................... 57,00............... 10%....... 51,00................. Activité 5–10 U/µl. Préparation d’ADNc (RT-PCR).................................... D34M-MLV reverse transcriptase................................................................................... FZ0303 *................... 50 rxns................ 176,50............. 20%....... 141,00............... Synthèse ADNc, séquençage (RT-PCR).................................................... NouveauEvaGreen dsDNA reagent 20X................................................................................ BI1790....................... 5 x 1 ml................ 201,00............. 15%....... 170,50............... Colorant pour PCR en temps réel.............................................................. D32EvaGreen dsDNA reagent 20000X.......................................................................... CA6770..................... 1 ml..................... 536,00............. 15%....... 455,50............... Conditionnement économique...................................................................Extraction aci<strong>de</strong>s nucléiquesDNA-mini Plasmid DNA Extraction Kit..................................................................... MP2711..................... 250 preps............ 175,50............. 20%....... 140,50............... 3-5 ml d’échantillon.................................................................................... NouveauDNA-midi Plasmid DNA Purification Kit................................................................... MP2690..................... 25 preps.............. 180,00............. 20%....... 144,00............... 30-50 ml high copy number plasmid.......................................................... NouveauDNA-maxi Plasmid DNA Purification Kit.................................................................. MP2680..................... 12 preps.............. 180,00............. 20%....... 144,00............... 100-150 ml high copy number plasmid...................................................... NouveauClonageT4 DNA ligase.......................................................................................................... 406680...................... 75000U................ 133,00............. 20%....... 106,00............... 150 U/µl..................................................................................................... D69Cellules compétentes DH5-alpha 10 7 ...................................................................... CE0981 *................... 5 x 200 µl............ 45,00............... 10%....... 40,50................. 10 7 cellules.................................................................................................Cellules compétentes DH5-alpha 10 9 ...................................................................... CE0982 *................... 5 x 200 µl............ 99,00............... 20%....... 79,00................. 10 9 cellules.................................................................................................X-Gal....................................................................................................................... UP40534M................ 1g........................ 55,00............... 20%....... 44,00................. ................................................................................................................... D73IPTG........................................................................................................................ UP84853C................. 1g........................ 16,00............... 20%....... 12,50................. ................................................................................................................... D71TransfectionUptiFectin-ON DNA transfection reagent................................................................. CK5060..................... 0,5 ml.................. 134,50............. 15%....... 114,00............... 375 transfections en plaque 24 puits......................................................... NouveauUptiFectin-OFF siRNA transfection reagent............................................................ CK5090..................... 0,5 ml.................. 124,50............. 15%....... 105,50............... 250 transfections en plaque 24 puits......................................................... NouveauGène rapporteurFirefly luciferase 1-step assay kit, 2h reading......................................................... FP-BX0320 *............. 100mL................. 495,00............. 20%....... 396,00............... 1000 tests en 96 puits................................................................................ NouveauRenilla luciferase assay kit...................................................................................... FP-BE7930 *............. 150 tests.............. 115,00............. 20%....... 92,00................. ...................................................................................................................Renilla luciferase assay kit...................................................................................... FP-BE7931 *............. 1000 tests............ 522,50............. 20%....... 418,00............... ...................................................................................................................Firefly and Renilla luciferase assay kit.................................................................... FP-BE7810 *............. 10mL................... 132,50............. 20%....... 106,00............... 100 tests.....................................................................................................Firefly and Renilla luciferase assay kit.................................................................... FP-BE7811 *............. 100mL................. 938,00............. 20%....... 750,00............... 1000 tests...................................................................................................* Frais <strong>de</strong> carboglace 32 e-mail interbiotech@interchim.comVisit our website : www.interchim.comA.3