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HISTOPATHOLOGY & CYTOLOGY
Histopathology specimens and tests require special
considerations as per the notes below:
Histopathology requestform
There is a special request form for Histopathology which
provides for details of anatomical site and clinical details.
Please contact the lab for the customised request forms.
Additional relevant information includes appearance and
distribution of lesions, duration of the condition, biopsy
sites, prior reports and treatments. It is advisable to
include the primary doctors contact details.
Bone specimens
A radiology report and radiological films must be
submitted for all bone specimens. The TAT for bone
specimens depends on fixation, and may extend to a
couple of weeks on average.
Histopathology charges
• Charges are determined by size of the tissue/
organ or number of biopsy specimens, which in
turn produces the tissue blocks, usually classified
as small (1-2 blocks), medium (3-5 blocks) or large
(>5 blocks).
• Some particular biopsies have special prices due to
the nature of processing and stains required
e.g. renal biopsies.
Specimen submission guidelines
• Place specimens in a wide necked container
(approved for use with formalin), with the ratio
of formalin to tissue>10:1
• Submit entire lesions and tumours with
adjacent excised tissue.
• For rapid fixation of larger lesions and tumours,
cut a section 0.5-1cm wide through the centre
of the specimen.
• Open hollow organs, such as intestines, prior
to placing them in a fixative.
• Small fragile specimens (bonemarrow, Tru-cut
liver or kidney) can be wrapped in a gauze
envelope so that they do not disintegrate
during transportation due to mechanical
disruption.
• Alert the lab on urgent samples.
Specimen container & formalin
It is recommended to follow the 10:10:10 rule which is
summarised as use 10% formalin at 10:1 ratio in terms
of volume of formalin to biopsy volume; and a tissue
section not more than 10mm thick to allow for easy
fixation.
Immunohistochemistry (Immunoperoxidase)
Immunostaining involves a special technique of
staining using various specific antibodies and detection
system with histopathology evaluation done on tissues
submitted. The charges depend on the number of
antibodies stains done. These are advised by the
pathologist after reviewing the case. Please contact the
lab for details.
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