24.05.2013 Views

La mappatura genica - Agraria

La mappatura genica - Agraria

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DOPPIA<br />

MARCATORI AFLP.<br />

estrazione DNA<br />

DIGESTIONE EcoRI e MseI<br />

5’AATTCCAC TCGT 3’<br />

3’GGTG AGCAT 5’<br />

LIGAZIONE adattatore (ds) EcoRI 3’ CATCTGACGCATGGTTAA 5’<br />

ADATTATORI adattatore (ds) MseI 5’TACTCAGGACTCTA 3’<br />

5’AATTCCACN NTCGTTA 3’<br />

3’TTAAGGTGN NAGCAAT 5’<br />

PREAMPLIFICAZIONE primer EcoRI “+1” A<br />

SELETTIVA primer MseI “+1” C<br />

A<br />

5’AATTCCACN NTCGTTA 3’<br />

3’TTAAGGTGN NAGCAAT 5’<br />

C<br />

5’AATTCCACA GTCGGTTA 3’<br />

3’TTAAGGTGT CAGCAAT 5’<br />

AMPLIFICAZIONE primer EcoRI “+3” AAC<br />

SELETTIVA primer MseI “+3” CAA<br />

AAC<br />

5’AATTCCACNNN NNNTCGGTTA 3’<br />

3’TTAAGGTGNNN NNNAGCAAT 5’<br />

AAC<br />

5’AATTCCACAAC TTGTCGGTTA 3’<br />

3’TTAAGGTGTTG AACAGCCAAT 5’

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