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SV40 Promoter/SV40 Origin 28-366<br />
b-Globin Intron 395-967<br />
T7 Promoter 1022-1040<br />
<strong>Myc</strong> Epitope Tag 1060-1087<br />
MCS 1089-1163<br />
SV40 polyA 1141-1274<br />
pUC origin 1414-2081<br />
ampicillin resistance (bla) ORF 2232-3089<br />
f1 origin 3659-3893<br />
f1 ori<br />
P SV40/SV40 ori<br />
beta-globin intron<br />
<strong>Myc</strong> Epitope Tag<br />
ampicillin<br />
<strong>pSG5</strong>-<strong>Myc</strong><br />
4.2 Kb<br />
pUC ori<br />
pT7<br />
SV40 poly A<br />
1089 EcoRI<br />
1095 BamHI<br />
1101 HindIII<br />
1107 KpnI<br />
1111 SacII<br />
1113 NotI<br />
1121 EcoRV<br />
1127 PstI<br />
1133 SacI<br />
1139 SmaI<br />
1145 XhoI<br />
1151 NheI<br />
1157 BglII
<strong>pSG5</strong>-<strong>Myc</strong>A<br />
<strong>Myc</strong> Epitope Tag EcoRI BamHI HindIII<br />
ATG GAA CAA AAG TTG ATT TCT GAA GAA GAT TTA GAA TTC GGA TCC AAG<br />
M E Q K L I S E E D L E F G S K<br />
SacII<br />
EcoRV<br />
KpnI NotI PstI SacI SmaI XhoI<br />
CTT GGT ACC GCG GCC GCG ATA TCC TGC AGG AGC TCC CCG GGC TCG AGG<br />
L G T A A A I S C R S S P G S R<br />
NheI<br />
BglII<br />
CTA GCA GAT CTT ATT AAA GCA GAA CTT GTT TAT TGC AGC TTA TAA<br />
L A D L I K A E L V Y C S L *<br />
<strong>pSG5</strong>-<strong>Myc</strong>B<br />
<strong>Myc</strong> Epitope Tag EcoRI BamHI<br />
ATG GAA CAA AAG TTG ATT TCT GAA GAA GAT TTA AGA ATT CGG ATC CAA<br />
M E Q K L I S E E D L R I R I Q<br />
SacII<br />
EcoRV<br />
HindIII KpnI NotI PstI SacI SmaI XhoI<br />
GCT TGG TAC CGC GGC CGC GAT ATC CTG CAG GAG CTC CCC GGG CTC GAG<br />
A W Y R G R D I L Q E L P G L E<br />
NheI<br />
BglII<br />
GCT AGC AGA TCT TAT TAA<br />
A S R S Y *<br />
<strong>pSG5</strong>-<strong>Myc</strong>C<br />
<strong>Myc</strong> Epitope Tag EcoRI BamHI<br />
ATG GAA CAA AAG TTG ATT TCT GAA GAA GAT TTA AAG AAT TCG GAT CCA<br />
M E Q K L I S E E D L K N S D P<br />
HindIII<br />
SacII<br />
EcoRV<br />
KpnI NotI PstI SacI SmaI XhoI<br />
AGC TTG GTA CCG CGG CCG CGA TAT CCT GCA GGA GCT CCC CGG GCT CGA<br />
S L V P R P R Y P A G A P R A R<br />
NheI<br />
GGC TAG<br />
G *<br />
BglII
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