MKMTR 1 (Z) MTR Antigens Blot - Milenia Biotec GmbH

- 8 -Short Instruction: M TR Antigens Blot(all sample volumes are given in µl)Steps Solution Sample Controls:negative/positive/cut-offPipet to the strips Wash Buffer 1000 µlIncubate for 5 min at room temperature(18-28 °C) on a shaker; do not aspiratethe solution!Addcontrol(undiluted)(500 µl forCSF)1000 µl- 10 µlAddsample(undiluted)10 µl -or add CSF(undiluted) 500 µl -Aspirate; wash the strips with 1000 µl washbuffer by mixing gently and aspirate againAdd 1000 µl of wash buffer to each stripand incubate for 5 min at RT on a shaker;aspirateRepeat this step onceAddIncubate for 60 min at RT on a shakerAspirate; wash the strips with 1000 µl washbuffer by mixing gently and aspirate againAdd 1000 µl of wash buffer to each stripand incubate for 5 min at RT on a shaker;aspirateRepeat this step onceAddIncubate for 5 – 15 min at RT on a shakerDecant and wash the strips twice with1000 µl destilled waterRemove the strips with tweezers and airdryon adsorbent paper for about 30 minAnalyze the band pattern using theevaluation sheetEnzymeConjugateSubstrateSolution1000 µl 1000 µl1000 µl 1000 µlFor a detailed description of the procedure see also page 5.MTR Antigens Blot (MKMTR)