CV De Rosa - Istituto di Chimica del Riconoscimento Molecolare ...

- Responsible of the project: “Molecular and structural studies of the Ser/Thrprotein kinase Stkp from human pathogen Streptococcus pneumoniae todevelop new antipneumonal drugs” in the framework of the ScientificCooperation Agreement CNR-CNRS 2012-2013.- Standard HPC grant of CASPUR 2012. Proposal title: “Structural studies ofthe Ser/Thr protein kinase StkP”- Standard HPC grant of CASPUR 2011. Proposal title: “In silico screening ofnew potential TCR/CollagenII-MHCII inhibitors against rheumatoidarthritis”- Standard HPC grant of CASPUR 2010. Proposal title: “A model ofdystroglycan’s domains by molecular dynamics simulations“.- Standard HPC grant of CASPUR 2009. Proposal title: “Molecular dynamicsinvestigation of the interaction between anticancer anthracyclines andNADPH-dependent cytosolic reductases“.• Partecipation to research projects:- Research projects (D1) funded by the Università Cattolica del S. Cuore diRoma, Faculty of Medicine, 2011, 2010, 2009, 2008, 2007, 2007, 2006,2005, 2004, 2003, 2002.- Research project of National Program of Antarctic Research (PNRA) 2004-2006: Evolution and molecular adaptation of the oxygen-transport system inpolar fish.- Research project of National Program of Antarctic Research (PNRA) 2002-2003: Physiological, biochemical and molecular bases of the evolutionaryadaptation of Antarctic Teleostei.- National MIUR COFIN-PRIN project 2003: Blood substitutes.- National MIUR COFIN-PRIN project 1996: Levels of protein structuralorganization: liveprotein.• Participation to national and international Scientific Congresses: She haspresented a number of abstracts either as first author or co-author, as posterand oral presentations.Page 3 - Curriculum vitaeof Maria Cristina De Rosa

ISI Articles 1. Tellone, E., Ficarra, S., Russo, A., Bellocco, E., Barreca, D., Laganà, G., Leuzzi, U.,Pirolli, D., De Rosa M.C., Giardina, B., Galtieri A. Caffeine inhibits erythrocytemembrane derangement by antioxidant activity and by blocking caspase 3activation. Biochimie. 94(2):393-402 (2012).2. Waite, A., De Rosa, M.C., Brancaccio, A, Blake, D.J. A Gain-of-GlycosylationMutation Associated with Myoclonus-Dystonia Syndrome Affects Traffickingand Processing of Mouse ε-Sarcoglycan in the Late Secretory Pathway. Hum.Mutat. 32(11):1246-58 (2011).3. De Rosa, M.C., Pirolli, D., Bozzi, M., Sciandra, F., Giardina, B., Brancaccio, A. Asecond Ig-like domain identified in dystroglycan by molecular modelling anddynamics. Journal of Molecular Graphics and Modelling. 29(8):1015-24 (2011).4. Andersen, O., De Rosa, M.C., Pirolli, D., Tooming-Klunderud, A., Petersen, P.E.,André, C. Polymorphism, selection and tandem duplication of transferringenes in Atlantic cod (Gadus morhua) - Conserved synteny between fishmonolobal and tetrapod bilobal transferrin loci. BMC Genet. 12:51 (2011).5. Pirolli, D., Carelli Alinovi, C., Capoluongo, E., Satta, M.A., Concolino, P., Giardina,B., De Rosa, M.C. Insight into a Novel p53 Single Point Mutation (G389E) byMolecular Dynamics Simulations. International Journal of Molecular Science.12(1):128-40 (2010).6. De Rosa, M.C., Giardina, B., Bianchi, C., Carelli Alinovi, C., Pirolli, D.,Ferraccioli, G. De Santis, M., Di Sante, G., Ria F. Modeling the Ternary ComplexTCR-Vβ/CollagenII(261-273)/HLA-DR4 Associated with RheumatoidArthritis. PLoS ONE 5(7): e11550 (2010).7. Colafranceschi, M., Giuliani, A., Andersen, Ø., Brix, O., De Rosa, M.C., Giardina,B., Colosimo, A. Hydrophobicity patterns and biological adaptation: anexemplary case from fish haemoglobins. OMICS. 14(3):275-81 (2010).8. Manconi, B., De Rosa, M.C., Cappabianca, M.P,, Olianas, A., Alinovi, C.C.,Mastropietro, F., Ponzini, D., Amato, A., Pellegrini, M. A new beta-chainhaemoglobin variant with increased oxygen affinity: Hb Roma[beta115(g17)Ala-->Val]. Biochimica et Biophysica Acta (BBA) – GeneralSubjects. 1800, 327-335 (2010).9. Vitali, A., Carelli Alinovi, C., De Rosa, M.C., Petruzzelli, R. Beta2-strand ofsalivary S cystatins: a "chemeleon sequence". Biochemical and BiophysicalResearch Communications. 387, 47-51 (2009).10. Andersen, O., Wetten, O.F., De Rosa, M.C., Andre, C., Carelli Alinovi, C.,Colafranceschi, M., Brix, O., Colosimo, A. Haemoglobin polymorphisms affectthe oxygen-binding properties in Atlantic cod populations. Proceedings of theRoyal Society B-Biological Sciences. 276, 833-41 (2009).11. De Rosa, M.C., Carelli Alinovi, C., Galtieri, A., Russo, A. and Giardina B.Allosteric properties of hemoglobin and the plasma membrane of theerythrocyte: new insights in gas transport and metabolic modulation. IUBMBLife 60, 87-93 (2008).12. De Rosa, M.C., Carelli Alinovi, C., Schininà, M.E., Clementi M.E., Amato, A.,Cappabianca M.P., Pezzotti, M., and Giardina, B. Hb Santa Clara (beta97His →Asn), a human haemoglobin variant: functional characterization andstructure modelling. Biochimica et Biophysica Acta (BBA)-Proteins andProteomics. 1774, 1299-306 (2007).13. De Rosa, M.C., Carelli Alinovi, C., Galtieri, A., Scatena, R., Giardina, B. Theplasma membrane of erythrocytes plays a fundamental role in the transportof oxygen, carbon dioxide and nitric oxide and in the maintenance of thereduced state of the heme iron. Gene. 398, 162-71 (2007).14. De Rosa, M.C., Carelli Alinovi, C., Russo A., Giardina, B. Binding modes of L35to alpha- and beta-semihemoglobins: structural insights into theinequivalence of alpha- and beta-subunits of hemoglobin. Biochemical andBiophysical Research Communications. 354, 720-726 (2007).Page 4 - Curriculum vitaeof Maria Cristina De Rosa

Page 5 - Curriculum vitaeof Maria Cristina De Rosa15. Verde, C., De Rosa, M.C., Giordano, D., Mosca, D., de Pascale, D., Raiola, L.,Cocca, E., Carratore, V., Giardina, B. and di Prisco, G. Structure, function andmolecular adaptations of haemoglobins of the polar cartilaginous fishBathyraja eatonii and Raja hyperborea. Biochemical Journal 389, 297-306(2005).16. Giardina, B., Mosca, D. and De Rosa, M.C. The Bohr effect of haemoglobin invertebrates: an example of molecular adaptation to different physiologicalrequirements. Acta Physiologica Scandinavica (ora Acta Physiologica) 182,229-44 (2004).17. Verde, C., Howes, B.D., De Rosa, M.C. Raiola, L. Smulevich, G., Williams, R.,Giardina, B., Parisi, E. and di Prisco, G. Structure and function of theGondwanian hemoglobin of Pseudaphritis urvillii, a primitive notothenioidfish of temperate latitudes. Protein Science 13, 2766-81 (2004).18. Giardina, E., Capon, F., De Rosa, M.C., Mango, R., Zambruno, G., Orecchia, A.,Chimenti, S. Giardina, B. and Novelli, G. Characterization of the Loricrin (LOR)Gene as a positional candidate for the PSORS4 psoriasis susceptibility locus.Annals of Human Genetics 68, 639-45 (2004).19. De Rosa, M.C., Castagnola, M., Bertonati, C., Galtieri, A., Giardina, B. From theArctic to fetal life: physiological importance and structural basis of an“additional” chloride-binding site in haemoglobin. Biochemical Journal 380,889-896 (2004).20. Corda, M., Tamburrini, M., De Rosa M.C., Sanna, M.T., Fais, A., Olianas, A.,Pellegrini, M., Giardina, B. and di Prisco, G. Whale (Balaenoptera physalus)haemoglobin: primary structure, functional characterisation and computermodelling studies. Comparative Biochemistry and Physiology B-Biochemistry &Molecular Biology 134, 53-62 (2003).21. De Rosa, M. C., Bertonati, C., Giardina, B., Di Stasio, E. and Brancaccio, A. Theeffect of anions on azide binding to myoglobin: an unusual functionalmodulation. Biochimica et Biophysica Acta (BBA) - Protein Structure andMolecular Enzymology 1594, 341-352 (2002).22. Clementi, M. E., De Rosa, M.C., Bertonati, C., Capo, C., Cataldi, E., Petruzzelli,R., and Giardina, B. Functional and structural properties of the hemoglobincomponents from Italian sturgeon (Acipenser Naccarii). Fish Physiology andBiochemistry 24, 191-200 (2002).23. Clementi, M.E., De Rosa, M.C., Bertonati, C., Capo, C., Cataldi, E., Petruzzelli,R. and Giardina, B. The hemoglobins of the “Fossil fish” Acipenser Naccarii:Functional properties and their structural basis. Hemoglobin 25, 447-51(2001).24. Pellegrini, M., Corda, M., Manca, L., Olianas, A., Sanna, M.T., Fais, A., De Rosa,M.C., Bertonati, C., Masala, B. and Giardina, B. Functional and computermodelling studies of hemoglobin from horse. The haemoglobin system of theSardinian wild dwarf horse. European Journal of Biochemistry 268, 3313-3320 (2001).25. Corda M., De Rosa, M.C., Pellegrini, M.G., Sanna, M.T., Olianas, A., Fais, A.,Manca, L., Masala, B., Zappacosta, B., Ficarra, S., Castagnola, M. and Giardina, B.Adult and fetal haemoglobin J-Sardegna [alpha50(CE8)His-->Asp]: functionaland molecular modelling studies. Biochemical Journal 346, 193-9 (2000).26. Manca, L., Cherchi L, De Rosa M.C., Giardina B. and Masala, B. A new,electrophoretically silent, fetal hemoglobin variant: Hb F-Calabria[→→118(GH1)Phe → Leu]. Hemoglobin, 24, 37-44 (2000).27. De Rosa, M.C., Sanna, M.T., Messana, I., Castagnola, M., Galtieri, A., Tellone, E.,Scatena, R., Botta, B., Botta, M. and Giardina, B. Glycated human hemoglobin(HbA1c) : Functional characteristics and molecular modeling studies.Biophysical Chemistry, 72, 323-335 (1998).28. De Rosa, M.C. and Berglund, A. A new method for predicting the alignment offlexible molecules and orienting them in a receptor cleft of known structure.Journal of Medicinal Chemistry 41, 691-698 (1998).29. Berglund, A., De Rosa, M.C. and Wold, S. Alignment of flexible molecules attheir receptor site using 3D descriptors and Hi-PCA. Journal of Computer-Aided Molecular Design 11, 601-612 (1997).30. Botta, B., Delle Monache, G., De Rosa, M.C., Seri, C., Benedetti, E., Iacovino, R.,Botta, M., Corelli, F., Masignani, V., Tafi, A., Gacs-Baitz, E., Santini, A. and Misiti,

D. Synthesis of C-Alkylcalix[4]arenes. 5. Design, synthesis, computationalstudies, and homodimerization of polymethylene-bridged resorc[4]arenes.Journal of Organic Chemistry 62, 1788-1794 (1997).31. Botta, B., Delle Monache, G., De Rosa, M.C., Scurria, R., Vitali, A., Vinciguerra,V., Menendez, P. and Misiti, D. Studies in cell suspension cultures of Cassiadidymobotrya. Part VI. The biotransformation of chalcones to aurones andauronols. Heterocycles, 43, 1415-1421 (1996).32. Botta, M., De Rosa, M.C., Di Fabio, R., Mozzetti, C., Santini, A. and Corelli, F.Experimental and theoretical investigations on the cephalosporin → → →→ →isomerization. Electronic Journal of Theoretical Chemistry, 1, 1-8 (1996).33. Botta, B., Delle Monache, G., De Rosa, M.C., Carbonetti, A., Baitz, E.G., Botta, M,Corelli, F. and D. Misiti. Synthesis of C-alkyl calix[4]arenes. 3. Acid-catalysedrearrangement of 2,6-dimethoxycinnamate prior to tetramerization tocalix[4]arenes. Journal of Organic Chemistry 60, 3657-3662 (1995).34. Delle Monache, G., De Rosa, M.C., Scurria, R., Vitali, A., Cuteri, A., Monacelli, B.,Pasqua, G. and Botta, B. Comparison between metabolite productions in cellculture and in whole plant of Maclura Pomifera. Phytochemistry, 39, 575-580(1995).35. Benedetti, E., Pedone, C., Iacovino, R., Botta, B., Delle Monache, G., De Rosa,M.C., Botta, M., Corelli, F., Tafi, A. and Santini, A. Structure and conformationof stereoisomeric C-isopropoxycarbonylmethylcalix[4]resorcinarenes.Journal of Chemical Research-S, 476-477 (1994).36. Botta, B., Di Giovanni, M.C., Delle Monache, G., De Rosa, M.C., Baitz, E.G., Botta,M., Corelli, F., Tafi, A., Santini, A., Benedetti, E., Pedone, C. and D. Misiti. Anovel route to calix[4]arenes. 2. Solution and solid state structural analysesand molecular modeling studies. Journal of Organic Chemistry 59, 1532-1541(1994).37. Delle Monache, G., De Rosa, M.C., Scurria, R., Monacelli, B., Pasqua, G.,Dall’Olio, G. and Botta, B. Metabolites from in vitro cultures of Cassiadidymobotrya. Phytochemistry, 30, 1849-1854 (1991).38. Botta, B., Vinciguerra, V., De Rosa, M.C., Scurria, R., Carbonetti, A., Ferrari, F.,Delle Monache, G. and Misiti, D. Studies in cell suspension cultures of Cassiadidymobotrya. Part III. The biotransformation of chalcones to flavones andbiflavanones. Heterocycles, 29, 2175-2183 (1989).39. Botta, B., Vinciguerra, V., De Rosa, M.C., Scurria, R., Iacomacci, P., Ferrari, F.,Delle Monache, G. and Misiti, D. Studies in cell suspension cultures of Cassiadidymobotrya. Part II. The biotransformation of chalcones to flavanones.Heterocycles, 29, 2167-2174 (1989).Page 6 - Curriculum vitaeof Maria Cristina De Rosa

ADDITIONAL INFORMATIONTeaching and Educational Activity:• Teaching for Università Cattolica del S. Cuore (UCSC), Faculty of Medicine:- 2011/2012: Propedeutica Biochimica, Corso di Laurea in BiotecnologieSanitarie, UCSC- 2011/2012: Propedeutica Biochimica, Corso di Laurea in Tecniche diLaboratorio Biomedico, UCSC- 2010/2011, 2008/09, 2006/07: Tecniche di modeling molecolare,Master Universitario di I Livello in Biologia Molecolare Clinica, UCSC.- From 2006/07 to 2010/11: Chimica Organica, Corso di Laurea inScienze e Tecnologie Cosmetologiche, UCSC.- From 2002/03 to 2007/08: Biochimica, Corso di Laurea inFisioterapia, UCSC.- 2001/02: Biochimica, Corso di Laurea in Tecniche della RiabilitazionePsichiatrica, UCSC.- 1999/00: Chimica e propedeutica biochimica, Scuola Diretta a FiniSpeciali per Tecnico di Igiene Ambientale e del Lavoro, UCSC.- From 2002/03 to 2005/06: Chimica Organica, Scuola Diretta a FiniSpeciali per Tecnici Cosmetici, UCSC- From 1998/99 to 2001/02: Chimica, biochimica e biochimica clinica,Scuola Diretta a Fini Speciali per Tecnici Cosmetici, UCSC.• Supervisor of master and graduate students of the Università degli Studidi Roma “La Sapienza” and of the “Università Cattolica del S. Cuore", Rome.• Referee for several peer-reviewed international journals.Page 7 - Curriculum vitaeof Maria Cristina De Rosa