sampaolese - Istituto di Chimica del Riconoscimento Molecolare

CURRICULUM VITAE
BEATRICE SAMPAOLESE
ADDRESS
Istituto di Chimica per il Riconoscimento Molecolare (ICRM-CNR),
Sezione di Roma, C/O Istituto di Biochimica e Biochimica Clinica -
Università Cattolica del Sacro Cuore,
Largo FrancescoVito n.1, 00168 Roma
CONTACTS
E-MAIL
Tel: 06 30155135 Fax: 06 30154309
Beatrice.Sampaolese@ICRM.CNR.it
PERSONAL INFORMATIONS
POSITION
Born in Rome on 1958
Tecnologist
EDUCATION AND TRAINING
Degree in Biological Sciences in 1983 at the University of Rome “La
Sapienza”
Qualification of biologist in 1985
PROFESSIONAL
APPOINTEMENTS
In 1990 employment as technician at the CNR “Centro di Studio per
gli Acidi Nucleici” directed by Professor Giorgio Tecce at the
Department of Molecular Biology and Genetics at the University "La
Sapienza"
Admission as member of “Società Italiana di Biofisica e Biologia
Molecolare” (SIBBM) in 1998
Principal Investigator in years 1999-2000-2001-2002 for a grant by
the National Research Council (CNR) about "DNA Superstructures
and nucleosomal organization”
Grant by the CNR about the "individual proposals training" served
out at the Prof. Bruno Samorì’s labo of the University of Bologna
for learnong the use of Atomic Force Microscopy.
Moving to the Rome Section directed by Professor Bruno Giardina of
the “Istituto di Chimica del Riconoscimento Molecolare” directed by
Professor Giacomo Carrea in 2003.
Employment in 2007 as Technologist for the same istitute.
ACADEMIC ACTIVITY
University of Rome “La Sapienza”

Supervisor with Prof. Maria Savino for an experimental
master thesis in Molecular Biology for degree in Biological
Sciences for academic year 1991-1992, submitted by Laura
Fantuzzi about “Role of N-terminal domains of Histones in
the positioning of Nucleosomes along DNA”.
Supervisor with Prof. Maria Savino for an experimental
master thesis in Molecular Biology for degree in Biological
Sciences for academic year 1993-1994, submitted by Bruno
Kropp about “DNA superstructures and terminal domains of
Histones in the positioning of Nucleosomes”.
Supervisor with Prof. Maria Savino for an experimental
master thesis in Molecular Biology for degree in Biological
Sciences for academic year 1996-1997, submitted by
Manuela Del Cornò about “Superstruttural features and DNA
Nucleosomal Centromeric Organization of Kluyveromyces
lactis and Saccharomyces cerevisiae
Supervisor with Prof. Maria Savino for an experimental
master thesis in Molecular Biology for degree in Biological
Sciences for academic year 1999-2000, submitted by Sonia
Mattei about “Nucleosome Organization on Saccharomyces
cerevisiae chromosome 6 and Kluyveromyces lactis
chromosome 1 centromeric DNA”
Seminars and practical exercises about "Topological Analysis
of a plasmid" for the subject in Organic Chemistry, held by
Prof. M. Savino for specialization course in Biotechnological
Applications in the Academic years ' 88-' 89, ' 90-' 91, ' 91-'
92, ' 97-' 98 and ' 98-' 99.
Lecture about "Centromeres of yeasts: Structural Studies" for
the course Physical Organic Chemistry, held by Prof. M.
Savino for specialization school in Biotechnological
Applications in the academic year ' 99-' 00.
Lecture and tutorial about "the study of DNA and chromatin
with the Atomic Force Microscopy (AFM)" for the course of
Organic Chemistry, held by Prof. M. Savino for the
specialization school in Biotechnological Applications for the
academic year ' 01-' 02.
Università Cattolica Del Sacro Cuore – Rome
Lecture about "Telomeres and Telomerase" for course of
Biochemistry and molecular biology for the degree in
medicine and surgery in the years 2006, 2007, 2008, 2009,
2010.
Lecture about "non-coding RNA" for course of Biochemistry
and molecular biology for the degree course in medicine and
surgery in the years 2008, 2009 and 2010.
Lecture about "RNA Interference" for course of Biochemistry
and Molecular Biology for the degree course in medicine and
surgery in 2007.
Lecture about "DNA structures" for course of Biochemistry
and molecular biology for the degree course in Biotechnology
Healthcare in years 2006, 2007, 2008, 2009 and 2010.

Università Cattolica del Sacro Cuore – Campobasso
Professor of “Human Biochemistry” in years 2009-2010 and
2010-2011 for the degree in “Biomedical Laboratory ”.
Professor of “Biochemistry” in years 2011-2012 and 2012-
2013 for the degree in “Biomedical Laboratory”.
PUBBLICATIONS
“b-amyloid decreases detectable endothelial nitric oxide
synthase in human erythrocytes: a role for membrane
acetylcholinesterase”. Misiti F, Carelli-Alinovi C,
Sampaolese B, Giardina B. Cell Biochem Funct. 2012
“Fibroblast apoptosis in a patient affected by lamellar
ichthyosis”.Tavian D, Colombo R, Misiti F, Ena P, Ena L,
Sampaolese B, Giardina B, Clementi ME. J Cutan Pathol.
2009 Apr;36(4):417-24.
“Globular structure of human ovulatory cervical mucus”.
Brunelli R, Papi M, Arcovito G, Bompiani A, Castagnola M,
Parasassi T, Sampaolese B, Vincenzoni F, De Spirito M.
FASEB J. 2007 Dec;21(14):3872-6.
“Protective effect of rhubarb derivatives on amyloid beta (1-
42) peptide-induced apoptosis in IMR-32 cells: a case of
nutrigenomic.” Misiti F, Sampaolese B, Mezzogori D, Orsini
F, Pezzotti M, Giardina B, Clementi ME. Brain Res Bull.
2006 Dec 11;71(1-3):29-36.
“Alzheimer's amyloid beta-peptide (1-42) induces cell death
in human neuroblastoma via bax/bcl-2 ratio increase: an
intriguing role for methionine 35”. Clementi ME, Pezzotti M,
Orsini F, Sampaolese B, Mezzogori D, Grassi C, Giardina B,
Misiti F. Biochem Biophys Res Commun. 2006 Mar
31;342(1):206-13
“Abeta(31-35) peptide induce apoptosis in PC 12 cells:
contrast with Abeta(25-35) peptide and examination of
underlying mechanisms”. Misiti F, Sampaolese B, Pezzotti
M, Marini S, Coletta M, Ceccarelli L, Giardina B, Clementi
ME. Neurochem Int. 2005 Jun;46(7):575-83.
“Recognition of the DNA sequence by an inorganic crystal
surface”. Sampaolese B, Bergia A, Scipioni A, Zuccheri G,
Savino M, Samori B, De Santis P. Proc Natl Acad Sci U S A.
2002 Oct 15;99(21):13566-70.
“Nucleosome organization on Kluyveromyces lactis
centromeric DNAs”. Mattei S, Sampaolese B, De Santis P,
Savino M. Biophys Chem. 2002 Jun 19;97(2-3):173-87.
“DNA superstructural features and nucleosomal organization
of the two centromeres of Kluyveromyces lactis chromosome
1 and Saccharomyces cerevisiae chromosome 6”. Del Cornò

M, De Santis P, Sampaolese B, Savino M. FEBS Lett. 1998
Jul 10;431(1):66-70.
“Influence of DNA superstructural features and histones
aminoterminal domains on mononucleosome and
dinucleosome positioning.” De Santis P, Kropp B, Leoni L,
Sampaolese B, Savino M. Biophys Chem. 1996 Nov 29;62(1-
3):47-61.
“Influence of DNA superstructural features and histone
amino-terminal domains on nucleosome positioning.” Kropp
B, Leoni L, Sampaolese B, Savino M. FEBS Lett. 1995 May
1;364(1):17-22.
“Influence of DNA topology and histone tails in nucleosome
organization on pBR322 DNA.” Buttinelli M, Leoni L,
Sampaolese B, Savino M. Nucleic Acids Res. 1991 Aug
25;19(16):4543-9.
“Cooperativity in nucleosomes assembly on supercoiled
pBR322 DNA.” Forte P, Leoni L, Sampaolese B, Savino M.
Nucleic Acids Res. 1989 Nov 11;17(21):8683-94.
“Specific interactions between DNA left-handed supercoils
and actinomycin D.” Leoni L, Morosetti S, Palermo C,
Sampaolese B, Savino M. Biophys Chem. 1989
Mar;33(1):11-7.
“Kinetic persistence of cruciform structures in reconstituted
minichromosomes.” Battistoni A, Leoni L, Sampaolese B,
Savino M. Biochim Biophys Acta. 1988 Jul 13;950(2):161-
71.
“Chiral discrimination between left-handed and right-handed
DNA supercoils by actinomycin D.” Savino M, Leoni L,
Morosetti S, Palermo C, Sampaolese B. Biochem Pharmacol.
1988 May 1;37(9):1869-70.
“Persistence of cruciform structure and preferential location
of nucleosomes on some regions of pBR322 and ColE 1
DNAs”. Caffarelli E, Leoni L, Sampaolese B, Savino M. Eur
J Biochem. 1986 Apr 15;156(2):335-42.
PUBBLICATION ON
BOOKS
Nova Publishers, Dental Composites, Editors: Frederick C. Calhoun
“Inhibition of Telomerase Activity in HL-60 Cell Line by
Methacrylic Monomers” (B. Sampaolese, A. Lupi, E. Di Stasio, G.E.
Martorana, G. Gambarini, B. Giardina, G. Nocca, Molecular
Recognition Chemistry Institute, C.N.R, Rome, Italy, and others)
RESEARCH ACTIVITY
At the CNR “Centro di Studio per gli Acidi Nucleici” C/O University
of Rome “La Sapienza”: participation in research programs of the
National Research Council CNR, the University "La Sapienza", the
MURST and Institut Pasteur Foundation-Cenci Bolognetti about to
the study of the relationship between structure and function of

chromatin. The research topics covered can be summarized as
follow:
Relationship between of superhelical density in
minichromosomes and stability of excited superstructures of
DNA.(1).
Specific interactions between antibiotics and left-handed
circular superhelical DNA (2, 4).
Preferential positioning of nucleosomes reconstituted
minichromosomes (3).
Study on superstructural features of some DNAs and their
interaction with histone octameric complex (5, 6, 7, 8).
Study of superstructural features of centromeric DNAs in
Kluyveromyces lactis and Saccharomyces cerevisiae
buddying yeasts and their interactions with regulatory
proteins (9, 10).
Study of chromatin and DNA by atomic force Microscopy,
AFM (11).
At the Rome Section of “Istituto di Chimica del Riconoscimento
Molecolare” at the Institute of Biochemistry and Clinical
Biochemistry, Università Cattolica del Sacro Cuore participation in
research programs of the National Research Council CNR and
Università Cattolica del Sacro Cuore research activity was about:
Expression of some genes involved in apoptotic course in
cells previously treated with -amyloid peptides (12, 13, 14).
Analysis by Atomic Force Microscopy in cervical mucus
during ovulatory phases (15).
Expression of some genes involved in apoptotic course of
fibroblasts in a patient with lamellar Ichthyosis (16).
Telomerase activity by TRAP assay of HL60 cells treated
with methacrylic monomers used in the dental practice
(pubblication on Book).
Study of erythrocytes biochemical modifications after
treatment with beta-amyloid (17).
MEETINGS, COURSES
AND WORKSHOPS
SIBBM (Società Italiana di Biofisica e Biologia Molecolare):
“”PHASING” DEL NUCLEOSOMA E STRUTTURE
CRUCIFORMI IN DNA CIRCOLARI”: E.Caffarelli,
C.Franzini, L.Leoni, B.Sampaolese e M.Savino (Cortona 11-
14 ottobre 1983).
“STABILITA’ DELLA STRUTTURA CRUCIFORME IN
DNA CIRCOLARI SUPERAVVOLTI IN PRESENZA DI
NUCLEOSOMI”: E.Caffarelli, C.Franzini, L.Leoni,
B.Sampaolese e M.Savino (Siena 21-25 ottobre 1984).
“ANALISI DELLA STRUTTURA DI SEQUENZE
PALINDROMICHE IN DNA CIRCOLARI ED IN SISTEMI
MODELLO DELLA CROMATINA MEDIANTE
IBRIDAZIONE CON OLIGONUCLEOTIDI SINTETICI”:

A.Battistoni, B.Sampaolese, L.Leoni e M.Savino (Cortona-
AR 1-4 ottobre 1985).
“dinamica di strutture cruciformI IN MINICROMOSOMI
RICOSTITUITI”: A.Battistoni, B.Sampaolese, L.leoni e
M.Savino (Pavia 13-15 ottobre 1986, (with oral presentation
of Dr Sampaolese).
“DISCRIMINAZIONE CHIRALE TRA “LEFT-HANDED”
E “RIGHT HANDED” SUPERCOILS DI DNA PRODOTTO
DALL’ACTINOMICINA D”: L.Leoni, S.Morosetti,
C.Palermo, B.Sampaolese e M.Savino (Padova 5-9 ottobre
1987).
“COOPERATIVITA’ DEL POSIZIONAMENTO DI
NUCLEOSOMI IN MINICROMOSOMI RICOSTITUITI SU
DNA DI pBR 322”: P.Forte, L.Leoni, B.Sampaolese e
M.Savino (Martina Franca-TA 12-14 ottobre 1988, (with oral
presentation of Dr Sampaolese).).
“Cooperatività nell’assemblaggio di minicromosomi:
influenze della densità di superelica, della temperatura e della
proteolisi”: M.Buttinelli, P.Forte, L.Leoni , B.Sampaolese e
M.Savino (Verona 16-19 ottobre 1989 with oral presentation
of Dr Sampaolese).
“INFLUENZA DELLA DENSITA’ DI SUPERELICA E
DELLA PROTEOLISI NEL PROCESSO DI
ASSEMBLAGGIO DI MINICROMOSOMI”: M.Buttinelli,
L.Fantuzzi, L.leoni, B.Sampaolese e M.Savino (Cortona-AR
15-17 ottobre 1990).
“RUOLO DEI DOMINI TERMINALI DEGLI ISTONI
NELLA STRUTTURA ED ORGANIZZAZIONE DI
NUCLEOSOMI IN DNA CIRCOLARI”: B.Sampaolese,
L.Fantuzzi, L.Leoni e M.Savino (Porto Conte-SS 2-5 ottobre
1991, with oral presentation of Dr Sampaolese).
“DIFFERENTE RUOLO DEI DOMINI N-TERMINALI
DEGLI ISTONI NEL POSIZIONAMENTO DI
NUCLEOSOMI SU DNA E NELLE INTERAZIONI
NUCLEOSOMALI”: B.Sampaolese, L.Fantuzzi, L.Leoni e
M.Savino (Bari 15-18 ottobre 1992, with oral presentation of
Dr Sampaolese).
“SUPERSTRUTTURE DEL DNA ED ORGANIZZAZIONE
DEL DINUCLEOSOMA: PREDIZIONE TEORICA E
DETERMINAZIONE SPERIMENTALE MEDIANTE
“FOOTPRINTING” CON Exo III E DNasi I” :
B.Sampaolese, L.Fantuzzi, B.Kropp L.Leoni e M.Savino
(Gubbio-PG 4-6 ottobre 1993).
“INFLUENZA DELLA SUPERSTRUTTURA DEL DNA E
DEI DOMINI AMINOTERMINALI DEGLI ISTONI
NELLA ORGANIZZAZIONE DI MONONUCLEOSOMI E
DINUCLEOSOMI”: B.Sampaolese, P.De Santis, B.Kropp,
L.Leoni e M.Savino (Montesilvano Lido-PE 2-6 ottobre
1995).
“ASPETTI STRUTTURALI DELLA CROMATINA
CENTROMERICA”: M.Del Cornò, L.leoni, B.Sampaolese e

M.Savino (Montesilvano Lido-PE 30 settembre-3 ottobre
1997).
“ASPETTI SUPERSTRUTTURALI ED
ORGANIZZAZIONE NUCLEOSOMALE DEL DNA
CENTROMERICO DI Kluyveromyces lactis E
Saccharomyces cerevisiae”: B.Sampaolese, M.Del Cornò,
P.De Santis e M.Savino (Montesilvano Lido-PE 1-4 ottobre
1998).
“IL POSIZIONAMENTO MULTIPLO CON FASE
ROTAZIONALE DI NUCLEOSOMI FORMATI SU DUE
DNA CENTROMERICI DI Kluyveromyces lactis E
Saccharomyces cerevisiae SUGGERISCE UN POSSIBILE
RUOLO DEI NUCLESOMI NELLA ORGANIZZAZIONE
DEL CINETOCORO”: B.Sampaolese, P.De Santis, S.Mattei
e M.Savino (Riva del Garda 2-6 ottobre 1999).
“ASPETTI SUPERSTRUTTURALI E FORMAZIONE DI
NUCLEOSOMI SU DI DUE DNA CENTROMERICI DI
Kluyveromyces lactis E Saccharomyces cerevisiae”:
B.Sampaolese, P.De Santis, S.Mattei e M.Savino (Riva del
Garda 30 settembre-4 ottobre 2000).
“I DNA CENTROMERICI DI Kluyveromyces lactis SONO
CAPACI DI ORGANIZZARE NUCLEOSOMI
TERMODINAMICAMENTE STABILI CARATTERIZZATI
DA POSIZIONAMENTO TRASLAZIONALE MULTIPLO
CON LA STESSA FASE ROTAZIONALE” : Sonia Mattei,
Beatrice Sampaolese, Pasquale De Santis e Maria Savino.
(Riva del Garda 21-25 settembre 2001).
“STUDIO STRUTTURALE MEDIANTE MICROSCOPIO
A SCANSIONE DI FORZA (SFM) DI UN FRAMMENTO
DI DNA ALTAMENTE CURVO DAL KINETOPLASTO
DEL PROTOZOO Crithidia fascicolata.” : Beatrice
Sampaolese, Anna Bergia, Giampaolo Zuccheri, Pasquale De
Santis and Maria Savino. (Riva del Garda 21-25 settembre
2001).
CIB (Consorzio Interuniversitario per le Biotecnologie).
“SISTEMI MODELLO DELLA CROMATINA: RUOLO
DEI DOMINI TERMINALI DEGLI ISTONI NELLA
STRUTTURA DEI NUCLEOSOMI E NELLE LORO
INTERAZIONI”: L.Fantuzzi, L.Leoni, B.Sampaolese e
M.Savino (Bari 14-15 maggio 1992).
“RELEVANCE OF DNA SUPERSTRUCTURES ON
PHYSICOCHEMICAL PROPERTIES AND IN
RECOGNITION MECHANISMS WITH PROTEINS”:
Cacchione Stefano, Leoni Luisa, Sampaolese Beatrice,
Savino Maria and Tufillaro Annamaria (Firenze 12-13
Giugno 1993).
SCI (Società Italiana di Chimica) “Chimica dei Sistemi Biologici”
group.
“RUOLO DEI DOMINI AMINOTERMINALI

DELL’OTTAMERO DEGLI ISTONI NELLA STABILITA’
E NEL POSIZIONAMENTO DI NUCLEOSOMI”: Bruno
Kropp, Luisa Leoni, Beatrice Sampaolese e Maria Savino
(Roma 9-11 dicembre 1993).
“A POSSIBLE ROLE OF HISTONE AMINOTERMINAL
DOMAINS ON NUCLEOSOME DYNAMICS”: Beatrice
Sampaolese, Bruno Kropp, Luisa Leoni and Maria Savino
(Bologna 25-27 gennaio 1995).
“THE ROLE OF A+T RICH YEAST CENTROMERIC
SEQUENCES IN DETERMINING DNA
SUPERSTRUCTURAL FEATURES AND NUCLEOSOME
ORGANIZATION”: Beatrice Sampaolese, Manuela Del
Cornò, Luisa Leoni and Maria Savino (Parma 15-17 dicembre
1997).
“THE HIGHLY BENT Crithidia fasciculate
KINETOPLAST DNA AS A SELECTIVE SEQUENCE
FOR STUDYING NUCLEOSOME ORGANIZATION AND
DNA CURVATURE AND FLEXIBILITY” : Beatrice
Sampaolese, Anna Bergia, Anita Scipioni, Giampaolo
Zuccheri, Pasquale De Santis, Bruno Samorì and Maria
Savino. (Certosa di Pontignano, SIENA, 8-10 febbraio 2002).
International Symposium on Structure and Dynamics of Nucleic
Acids and Membranes. “CRUCIFORM STRUCTURES AND
NUCLEOSOME “PHASING” IN CIRCULAR SUPERCOILED
DNA” Savino, M., Leoni, L., Caffarelli, E., Franzini, C. and
Sampaolese, B. (Roma 23-27 aprile 1984).
International Congress on Nucleic Acids Interactions. “SPECIFIC
INTERACTIONS BETWEEN ACTINOMYCIN D AND
NEGATIVE DNA SUPERCOILS”: M.Savino, L.Leoni, S.Morosetti,
C.Palermo and B.Sampaolese (Padova 1987).
19th FEBS Meeting. “COOPERATIVITY IN INTERACTION OF
THE HISTONE OCTAMER WITH pBR 322 SUPERCOILED
DNA”: P.Forte, L.Leoni, B.Sampaolese and M.Savino (Roma 1989).
6th Congresso Nazionale sulle Proteine. “INFLUENCE OF DNA
TOPOLOGY AND HISTONES TAILS IN NUCLEOSOME
ORGANIZATION ON pBR 322 DNA”: Beatrice Sampaolese,
Memmo Buttinelli, Laura Fantuzzi, Luisa Leoni and Maria Savino.
(Trieste 1991).
11th International Biophysics Congress. “DNA
SUPERSTRUCTURES: RELEVANCE ON PHYSICOCHEMICAL
PROPERTIES AND PROTEIN RECOGNITION”: S.Cacchione,
P.De Santis, B.Sampaolese, M.Savino. (Budapest-Hungury 25-30
luglio 1993).
“The year of Luis Pasteur International Symposium : Molecular
Control of Development” Istituto Pasteur-Fondazione Cenci
Bolognetti (Roma 1995).

Gordon Research Conference on “Plasmid and Chromosome
Dynamics” . “SUPERSTRUCUTRAL FEATURES AND
NUCLEOSOME ORGANIZATION IN BUDDING YEAST
CENTROMERIC SEQUENCES”: B.Sampaolese, P.De Santis,
S.Mattei and M.Savino (New London, NH, USA 1-6 agosto 1999).
EMBO Workshop on “Centromeres, Kinetochores and Spindle
Interactions”. “SUPERSTRUCTURAL FEATURES OF TWO
BUDDING YEAST CENTROMERIC DNAs”: B.Sampaolese,
S.Mattei, P.De Santis and M.Savino (EMBL, Heidelberg, Germany
14-18 Ottobre 2000).
International Workshop on “DNA-based molecular construction”.
“RECOGNITION OF THE DNA SEQUENCE BY AN
INORGANIC CRYSTAL SURFACE”: Bruno Samorì, Beatrice
Sampaolese, Anna Bergia, Anita Scipioni, Giampaolo Zuccheri,
Maria Savino, Pasquale De Santis. (Jena, Germany 23-25 Maggio
2002).
S.I.B. (Società Italiana di Biochimica).
“PROSTAGLANDINS AS MODULATOR OF HUMAN
CYTOSOLIC ANTHRACYCLINE REDUCTASE”: Andrea
Silvestrini, Alvaro Mordente, Beatrice Sampaolese, Bruno Giardina,
and Elisabetta Meucci. (Riccione, 28 Settembre-1 Ottobre 2004).
International Congress on Alzheimer Disease (ICAD)
“Changes In Erythrocyte Deformability In Alzheimer's Disease: A
Role For Nitric Oxide Synthase”: Francesco Misiti, Beatrice
Sampaolese, M.Elisabetta Clementi, Bruno Giardina (July 11-16
2009 Vienna, Austria)
Summer School on “Unusual DNA structures and their possible
biological implications”, Trieste September 1990
INSERM Atelier de Formation n. 95 on “Methods and Approaches to
Chromatin Dynamics” theoretical phase (Paris, France on January
1998) with selected presentation of own research project results.
Selected for practical phase with Journal Club presentation held to
Prof. Miguel Beato's lab at “Institute of Molecular Biology and
Tumor Research”, IMT (Marburg, Germany on february 1998)